In this paper, garbage-composting microflora were analyzed by cultivation and molecular biological approaches (FISH method, PCR-DGGE method) . As a result, thirty-four strains belonging to a Gram positive Low G+C content group were isolated by cultivation. Predominant microbes were determined to be eubacteria belonging to a Gram positive Low G+C group by the FISH method. Moreover,
Geobacillus thermodenitrzficansand
Geobacillus toebiiwere proven to be highly capable of decomposing organic substances in an early thermophilic stage. Although
Ureibacillus thermosphaericusor neighbor species were not isolated by cultivation, the molecular biological approach suggests that they exist in compost.
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