Journal of Environmental Conservation Engineering
Online ISSN : 1882-8590
Print ISSN : 0388-9459
ISSN-L : 0388-9459
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Volume 38, Issue 2
Displaying 1-3 of 3 articles from this issue
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  • Measurement of Estrogenic Activity with NRL/TIF2-BAP Assay
    Hiroshi TSUNO, Heqing ZHANG, Masanori SAKAMOTO, Harumi YAMADA
    2009 Volume 38 Issue 2 Pages 111-119
    Published: February 20, 2009
    Released on J-STAGE: June 15, 2011
    DOIhttps://doi.org/10.5956/jriet.38.111
    JOURNAL FREE ACCESS
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    A Nuclear Receptor Ligand (NRL) /TIF2-BAP assay combined with the solid phase extraction procedure with silica cartridge clean-up shows high sensitivity in determination of E1,E2, and E3 without inhibition of color nor natural organic matter. The values of relative binding affinity of E1,E3, BPA and NP, which is defined as the relative estrogenic activity compared with E2 were 1.7, 2.7, 0.018 and 0.015, respectively, based on the unit with nM. Acceptable recovery efficiencies (>60%) were obtained from the experiments spiked with E2 in sewage effluent with C.V. less than 20%. However, in the NRL/TIF2-BAP assay and sum of individual EDC activity measured by GC-MS analysis, it is shown that there is no significant difference between results obtained in the Expo demonstration plant, advanced sewage treatment processes, and through ozonation of primary effluent, except in their original sewage samples. It is concluded that NRL/TIF2-BAP assay is useful to evaluate estrogenic activity in environmental water and sewage treatment. Key Words : NRL/TIF2-BAP assay, endocrine-disrupting chemicals, estrogenicity, estrone, 17β-estradiol.
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  • Removal of Cadmium from Sea Scallop Processing Waste by Washing with Weak Acid for Low Cost Recycle of Useful Biological Components
    Huifeng REN, Ryoji FUKUDA, Atsushi KOBAYASHI, Hideaki ENDO, Tetsuhito ...
    2009 Volume 38 Issue 2 Pages 120-125
    Published: February 20, 2009
    Released on J-STAGE: June 15, 2011
    DOIhttps://doi.org/10.5956/jriet.38.120
    JOURNAL FREE ACCESS
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    Processing waste of sea scallops, of which 500-550 thousand tons are cultured annually in Japan, contains highly bio-accumulated cadmium and is a difficult industrial waste to dispose of safely. Though a system combining strong acid soaking and electrolysis has been developed to remove cadmium, the introduction of this type of system to small and medium-sized enterprises is difficult because of the large amount of required capital investment.There are some points to be improved in the system; one of the most complicated being the manual separation of the hepatopancreas before automatic processing.As a part of system development for this purpose, the authors have previously reported fundamental conditions using a weak acid washing method. However, there have been significant difficulties, such as a large amount of waste solution being generated in the process, and the challenge of the manual separation of the hepatopancreas. In this paper, we have described the optimum conditions to considerably decrease the volume of waste fluid, as well as the elimination of the manual separation of the hepatopancreas, by the combination of ordinary techniques and the weak acid washing method already reported.
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