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Y. NISHIOKA, K. SAKAGAMI, T. MATSUNAGA, M. MIYAZAKI, J. MATSUOKA, Y. S ...
1985 Volume 14 Issue 1 Pages
361-365
Published: February 15, 1985
Released on J-STAGE: October 07, 2011
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An extracorporeal circulation system for plasma exchange (PE) was developed in cancer bearing rats model. After removing of various immunosuppressive factors in the sera of cancer bearing hosts using PE, adequate immunochemotherapy was performed. We studied the roles of PE in augmenting efficacy to immunochemotherapy and the changes of immunosuppressive factors in the sera. Immunosuppressive substance, AFP level and PHA inhibitory factors were increased gradually in the sera along with the tumor development from fifth or sixth day after tumor inoculation. After P E performed PHA inhibitory factors and AFP level in the sera were adequately decreased compared with those before PE performed. On the case that chemotherapy was administered after PE, the survival of those group was significantly better than that of chemotherapy alone group.
On the case that OH-1, the lymphokine, which possess anti-tumor effect, was administered after PE, the anti-tumor effect of OH-1 alone group was significantly better than that of control group, and these effect of PE+OH-1 group was further better than that of OH-1 alone group. The effectiveness of immunochemotherapy in cancer bearing host is augmented by removal of the immunosuppressive factors using PE.
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-regarding simultaneous use of DFPP and adsorbent-
M. MIYAZAKI, K. SAKAGAMI, J. MATSUOKA, Y. NISHIOKA, T. MATSUNAGA, [in ...
1985 Volume 14 Issue 1 Pages
366-371
Published: February 15, 1985
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we have reported that the immunosuppressive factors, those existing in the high molecular weight fraction are removable by means of the double filtration plasmapheresis (DFPP) This time we report the result of an experiment to remove immunosuppressive factors existing in the low molecular waight fraction by the use of porous glass beads adsorbents.
16 adsorbents were used. each thereof having different surface charge and pore size. the immunosuppressive activity of serum was measured by the use of PHA-blastogenesis. The serum treated with adsorbent 100Å in pore size and with its surface coated with -NH
2 showed the highest reduction of immunosuppressive effect. It was discovered the decrease of immunosuppressive effect was visible already after an incubation time of 5 minutes and at serum adsorbent ratio of 10. Regarding the adsorbability for IAP. 43% decrease of IAP concentration was seen from the initial level of 931ng/ml. Thus, it was disclosed that removal of immunosuppressive factors in the low molecular weight fraction is feasible by the use of adsorbent. It is supposed thet removal of immunosuppressive factors is attainable more effectively by simultaneous use of adsorbent and DFPP.
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
1985 Volume 14 Issue 1 Pages
372-375
Published: February 15, 1985
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We utilized ‘NaCl-preciptation method’ to remove serum blocking factors by use of modification of plasmapheresis system. The plasma was separated from formed elements by a diacetale cellulose membrane, and 2.5l of plasma was collected with 800 grams of NaCl in a plastic bag. The precipitation, which contains the blocking factors, was formed in the plastic bag, and the bag was stored in 4°C for three nights. The precipitation was removed by a filter membrane, and then saturated NaCl was removed by H. D. In the 1st treatment, 5% Albumin solution was used as replacement fluid, and from 2nd treatment, globulin reduced own plasma was used as replacement fluid. We treated three cancer patients and eight runs have been done to cancer patients over four weeks with no significant side effect. The tumor decreared in two patients, and at the sametime there were inprovements in the general conditions.
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T. YOSHIOKA, T. TANI, T. OKA, K. HANASAWA, Y. ENDO, A. FUKUTANI, Y. NA ...
1985 Volume 14 Issue 1 Pages
376-380
Published: February 15, 1985
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Serum of cancer patients has been shown to supress several immune reaction including PHA-induced blastformation of normal lymphocyte (PHA). When serum of cancer patient were preparated by heating at °56C for 30 minutes in vitro, there was a remarkable increase in PHA. The effects and safety of 56°C plasma thermopreparation which was adapted plasmaperfusion under membrane plasmapheresis in order to treat experimental endotoxin shock on dogs.
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H. YURA, T. DAIDO, Y. TAKAHASHI, T. AKAIKE, R. AOGAKI
1985 Volume 14 Issue 1 Pages
381-384
Published: February 15, 1985
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In order to separate lymphocytes subpopulation (T and B cells) from rat mesentric lymph-node, adhesion behaviors of T and B cells onto electrode surface polarized electrochemically were investigated. On setting potential at -1000mV vs S.C.E., T cells attached to electrode surface selectively, and B cells were recovered in high yield as a non-adherent cell, particularly the yield of recovery B cell was about 100% under this condition after 20min.
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[in Japanese]
1985 Volume 14 Issue 1 Pages
385
Published: February 15, 1985
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Y UEDA, M UEDA, N YAMADA, Y IKEDA, Y YAGIRI
1985 Volume 14 Issue 1 Pages
386-389
Published: February 15, 1985
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Many kinds of extracorporeal systems, for the therapeutic application of extracorporeal circulation have been established. The treatment of patients with the bleeding tendency necessitates the careful selection of an anticoagulant in order to carry out circulation safely. Recently in hemodialysis, gabaxate mesilate (FOY), a serine protease inhibitor has been used as an anticoagulant for the patient with the bleeding tendency. It is special features include a short half life (about 2 minutes) and dialyzability. We successfully performed centrifugal plasma exchange using an IBM-2997 with gabaxate mesilate as an anticoagulant. And we established the equation to calculate the optimal dosage of gabaxate mesilate for extracorporeal circulation. t=0.028C-0.06 (r=0.986).
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F. TANAKA, H. KOMORIYAMA, S. HAMABE, M. HAMAGUCHI, S. KUBOTA, T. YONAH ...
1985 Volume 14 Issue 1 Pages
390-393
Published: February 15, 1985
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It is necessary to use the anti-coagulants during the extracorporeal circulation such as artificial liver support system, Heparin is the most common anticoagulant, We used gabexate mes ilate (GM: FOY®) with plasma separation for the extracorporeal circulation because of the inhibitor of blood coagulating enzymes, Continuous injection of FOY® in the rate of 100mg/kg/Hr was performed on the arterial line of the plasmaxs. Plasma from 10 of 11 animals were separated without any troubles. The platelet count and white blood cell count fell 37% and 42% of the initial count for 30min. respectively. However, they increased to 100% and 55% of the initial count for 120min. respectively. Pi, Δp, and Transmembrane pressure (TMP) were within normal levels during the extracorporeal circulation. however, delayed activated coagulation time (ACT) was only showed during the extracorporeal circulation. These results suggest that use of FOY® during the extracorporeal circulation for plasma separation was effective on the anti-coagulant actions.
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Y UEDA, M UEDA, Y YAGIRI, Y IKEDA, N YAMADA, T MIYAHARA, A NAKANO, T K ...
1985 Volume 14 Issue 1 Pages
394-396
Published: February 15, 1985
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Recently we established new system of plasmapheresis, post centrifugal plasmapheresis (PCPP). In this system, we combined a centrifugal blood cell separator and membrane type plasma component separator. In order to carry out PCPP safely and automatically in a closed circuit, we developed the KM-8300, originally developed as a secondary plasma processing monitor to PCPP. The feasibility of the KM-8300 system for plasmapheresis is high and includes secondary plasma processing by filters, using IBM, Haemonetics or Fenwal equipment. PCPP requires no special blood access and PCPP can perform lymphoplasmapheresis. Centrifugal plasma separation does not induce compliment activation. PCPP is a new available system of plasmapneresis.
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S MASTUBARA, Y ASANUMA, K OUCHI, T FUJIYA, M ABE, K KOYAMA, T SATO
1985 Volume 14 Issue 1 Pages
397-400
Published: February 15, 1985
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COBE-TPE system was evaluated clinically applying 121 sessions of plasma exchange procedures for 18 patients. At various blood flow rates ranging from 50 to 130ml/min, the system generated high plasma separation rate averaging 33ml/min for a mean of 2.5L exchange volume. The biocompatibility study showed minimal changes of both white blood cell and platelet counts. In partial plasma exchange the complement C
3 showed a consistent decrease in accordance with the decline of other plasma solutes such as Ig-G and lipids. On the other hand, C
4 level demonstrated further significant decrease. These data suggest the system mainly activates the classical pathway and does not affect the change of white blood cell count occuring in other extracorporeal systems.
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T. MATSUMOTO, T. DOHI, H. FUNAKUBO, T. USIDA, A TAKEUCHI
1985 Volume 14 Issue 1 Pages
401-404
Published: February 15, 1985
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A blood purification system is being developed which is able to be flexibly adapted to various blood purification therapies such as hemofiltration, plasmapheresis which have been widely utilized in the treatment of blood toxification and diseases caused by renal or hepatic failure. The system is composed of 5 roller-pumps (2 DC-motor pumps, 3 pulse-motor pumps), 2 flow meters and 6 pressure sensors, controlled or supervised by a micro-computer interfaced with a personal computer having a CRT, disk drive unit and printer. The system is being evaluated through in vitro and ex vivo experiments in order to develop programs for the most suitable therapies corresponding to each disease.
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[in Japanese]
1985 Volume 14 Issue 1 Pages
405
Published: February 15, 1985
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M. UENO, T. SHIMOYAMA, James W. SMITH, Paul S. MALCHESK, Y. NOSE
1985 Volume 14 Issue 1 Pages
406-409
Published: February 15, 1985
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Hematological changes in cryofiltration were studied in twelve patients with rheumatoid arthritis. Hematological changes occuered in individual treatment, however, no symptom due to thease phenomena were noted. Hematological parameters were stable in long term treatment. These results indicate that cryofiltration is safe to perform.
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Y. ABE, J. W. SMITH, P. S. MALCHESKY, Y. NOSE, K. TAKEBE
1985 Volume 14 Issue 1 Pages
410-412
Published: February 15, 1985
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The technique of cryofiltration has been applied in the treatment of cryoglobulinemia. Good clinical improvement has been demonstrated accompanied by decreases of cryoglobilin levels. The combination therapy with cytotoxic drugs performed at adequate frequency and volume processed maintained relief of symptoms and serologic improvement. The cryofilter demonstrates good selectivity for cryoglobulin removal in cryogel, comfirmed by electrophoretic analysis of both the cryogel and filtrate from the cryofilter used clinically. Plasma cryofiltration appears to be a safe alternative to plasma exchange, requiring no replacement fluid.
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A SUEOKA, T MIYAHARA, K TAKAKURA, Y UEDA
1985 Volume 14 Issue 1 Pages
413-416
Published: February 15, 1985
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Filtration characteristics of EVAL secondary filters were studied in vitro tests by using diseases plasma with varying plasma composition. The filtrate volume processed was dependent upon plasma types. Stable and constant sieving for each solutes were achieved independent of transmembrane pressure and filtrate volume. Transmembrane pressure showed the good correlation to bulk concentration of total protein trapped in the filters irrespective of plasma types and membrane types. The filtration of flow direction from outside to inside of hollow fibers gave lower filtrate volume and unstable sieving to increasing transmembrane pressure compared to that from inside to outside. It is suggested that the microstructure of EVAL_ membrane would connect to filtration characteristics of EVAL filter.
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T. KOSHOJI, N. MINAGAWA, H. YAMAMORI, A. KAWAI, M. KOHYAMA, O. ODA, N. ...
1985 Volume 14 Issue 1 Pages
417-420
Published: February 15, 1985
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Using microporous polyolefine hollow fiber, EHF and KPF (Mitsubishi Rayon Co.), the 2nd filters for double filtration were developed. Types of the membrane can adequatedly be chosen based on molecular size of the solute. In other words, separation of plasma protein is directed by membrane's pore size represented by bubble point, i. e, separation of albumin from globulin was effective in use of fiber with membrane of a high bubble point. Net volume of plasma obtainable under a T. M. P. was primarily controlled by filtration methods, of cause by changes of bubble point, effective surface area etc. That is, recycling method gave increased volume of plasma when recycling . rate was increased and partial discarding method under a constant pressure gave a stabilized S. C. and flow rate in a function of running time.
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Y. IMAI, S. SHINAGAWA, S. NAKAGAWA, K. HIDA
1985 Volume 14 Issue 1 Pages
421-423
Published: February 15, 1985
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With expanded application of plasmapheresis (PP) to various diseases, a shortage of supply in replacement fluid is a problem. Although so-called DPPP intends to reduce the consumption of fluid by reprocessing the filtrate, many system still requires some amount of fluid infusion. To overcome this demerit, an automatic back wash system was developed. With this PP-DFPP system, 25 PP were performed in 3 patients with rheumatic disease. Reduction of RAHA, complements and immunogloburins achieved almost same degree with currently available other systems. PP without replacement fluid is possible.
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M. KOHYAMA, O. ODA, K. OHTA, N. YAMANAKA, K. MAEDA, T. KOSHOJI, A. KAW ...
1985 Volume 14 Issue 1 Pages
424-426
Published: February 15, 1985
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The evaluation for the polypropyrene plasma filter was carried ait by in vivo studies on bioconpatibility and permeability. The results were as follaas: 1. No ranarkable changes in blood cells and clinical side effects such as fever. 2. The sieving coefficients for total protein albumin, IgG, IgA and IgM were 0.47, 0.55, 0.44, 0.26 and nearly 0, respectively. We conclude that this plasma filter has sufficient efficacy' and biocanpatibility for double-filtration.
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T. SEKIGUCHI, T. AKIZAWA, S. KOSHIKAWA, K. MATSUZAWA, R. HASEGAWA, E. ...
1985 Volume 14 Issue 1 Pages
427-430
Published: February 15, 1985
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We clinically evaluated the newly developed plasma filter which was composed of anisotropic porous follow fiber membrane with outer surface having smaller pore size than that of inner one. In this device, separated plasma flowed from outer surface of membrane to inner surface. The filter sharply removed the large molecular weight protein such as IgM and it also separated IgG with sufficient collection of albumin. The sieving coefficient of plasma components, transmembrane pressure and filtrated volume were maintained almost constantly during 4hr-treatment. The stableand long standing. filtration capacities were the most remarkable characteristics of this membrane. We conclude that this plasma filter can be widely available for clinical application.
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[in Japanese]
1985 Volume 14 Issue 1 Pages
431
Published: February 15, 1985
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J. MINAKUCHI, T. AGISHI, I. KANEKO, Y. HASUO, K. OHTA, A. SUEOKA, A. I ...
1985 Volume 14 Issue 1 Pages
432-434
Published: February 15, 1985
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Blood component transfusion has become popular in recent years with an intention to avoid unnecessary usage of blood components. However, plasma fraction products always tend to be deficient compared to those of cellular fraction. Donation plasmapheresis is of importance in this situation and the minimum requirement for donation plasmapheresis was collection of 400ml of plasma in 30min with single needle venipuncture. This study describes comparative ex vivo evaluation of the mimic membrane donation plasmapheresis utilizing “collected blood bypass return” system. About 480ml of plasma were collected in from 33 to 55min by this system and concentration ratio in TP of the total collected plasma was from 0.67 to 0.91. Advantage of “collected blood bypass return” system lies in absence of plasma dilusion in spite of using substitution fluid.
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M. MINESHIMA, T. AGISHI, K. OTA, H. IKEDA, T. TOMONO, T. OOSAWA, M. WA ...
1985 Volume 14 Issue 1 Pages
435-438
Published: February 15, 1985
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Plasma collection by membrane separation should be operated under several restricted conditions, i. e., blood flow rate, treatment time, safety for donor and cost. In this paper, performances of plasma separator were evaluated in vitro experiments with bovine blood. Relation between blood side pressure drop, filtration flux, hematocrit and inlet flow rate were elucidated by multiple regression analysis. Four types of collection modality, which were consisted of single pass recycle, semi-continuous recycle and semi-continuous recycle with containing blood bag were examined by compartment model and material balance equations.
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Z. YAMAZAKI, [in Japanese], [in Japanese], [in Japanese], Y. IDEZUKI, ...
1985 Volume 14 Issue 1 Pages
439-442
Published: February 15, 1985
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A simple, rapid and safe procedure for donor plasmaphresis, using membrane plasma separator and peumatic control unit (PCU), has been experimentally investigated. The procedure was easily performed and 300ml of plasma was obtained within 30 minutes, however, the total protein concentration in the filtrated plasma was 70-80% of that in the blood.
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K. ICHIKAWA, H. ICHIKAWA, N. INOUE, Z. YAMAZAKI, [in Japanese], Y. IDE ...
1985 Volume 14 Issue 1 Pages
443-445
Published: February 15, 1985
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Basic studies on donor plasmapheresis through a single venous blood access, using membrane plasma separator and recirculation circuit. To improve an efficiecy and to increase the protein concentration in the filtrated plasma obtained by the previous method, 2 stages plasma collection has been experimentally investigated. An initially collected plasma (100 or 200ml) was reserved as infusion fluid for an animal after the donor plasmapheresis and the circuit was improved.
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M. TAMURA, M. KASAI, H. HATTORI
1985 Volume 14 Issue 1 Pages
446-450
Published: February 15, 1985
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In order to reduce donors' restriction and pain, a new plasma collection system requiring only one needle (DP-1S) was developed by us. With a cellulose-tri-acetate hollow fiber type plasma separator (PEX-25). our system was clinically evaluated and proved to be safe and effective every procedure was carried out without showing any complication of the donors or abnormal laboratory findings. The duration of the precedure was within 1 hour for collecting 500ml o f cell-free and hemolysis-free plasma.
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M. TAMURA, M. KASAI, K. FUKUI
1985 Volume 14 Issue 1 Pages
451-459
Published: February 15, 1985
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Establishment of the new technology for donor plasmapheresis has been desired to meet recent increasing demand of plasma and its derivatives for medical treatments. The combination of newly developed membrane separator made of cellulose-tri-acetate hollow fiber and especially designed plasma collection system whose extracorporeal volume was 98ml made it possible to carry out donor plasma-pheresis successfully, i. e. safely and efficiently. Forty-two procedures of donor pheresis by our system showed that collection of 500ml plasma was executed within 30-40minutes (plasmapheresis time) and with good ‘recovery ratio’ and sufficiently high sieving coefficients.
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[in Japanese]
1985 Volume 14 Issue 1 Pages
460
Published: February 15, 1985
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-Evaluation of membrane plasma separator-
H. IKEDA, H. HAYAMIZU, T. TOMONO
1985 Volume 14 Issue 1 Pages
461-464
Published: February 15, 1985
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For the purpose of donor plasmapheresis, the membrane plasma separators were evaluated from the views of performance and safety of modules. The results of in vitro experiments indicated that the stable separation at low blood flow rate of 50ml/min could be achieved at plasma filtrate rate of 15ml/min. The sieving coefficients and recoveries of plasma proteins were relatively high, over 0.95, except that the recovery of anti-coagulant activity (FVIII) was lower than 0.8. The further investigations must, therefore, be performed to improve the recovery of FVIII.
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-Investigations of plasma collecting systems-
H. IKEDA, H. HAYAMIZU, T. TOMONO
1985 Volume 14 Issue 1 Pages
465-468
Published: February 15, 1985
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The theoretical analysis was performed with regard to plasma collecting systems including i) continuous flow method, ii) semi-continuous flow method and iii) intermittent flow method. The effects of blood recirculation on hmatocrit at module inlet were also investigated. The results of in vitro experiments indicated that increase of flow ratio (RRQR/QB) resulted in rising of pressure loss (ΔP) of modules. The optimal condition of blood recirculation was determined as QR=50ml/min and QM=100ml/min at QB=50ml/min.
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M UEDA, Y UEDA, N YAMADA, Y IKEDA, Y YAGIRI
1985 Volume 14 Issue 1 Pages
469-471
Published: February 15, 1985
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This paperr describes our attempts to remove toxic substances from the patients plasma ex vivo, separation i t from the extracorporeal circulation, and immediately freeze the refined plasma before reusing it as the replacement fluid in the next plasma exchange. we refer to this system of plasma exchange as processed autol ogous fresh-frozen plasma (PAFFP). We regard plasma exchange using PAFFP as a very comfortable physiological and safe procedure for patients. When we finish to perform PCPP or DFPP, we collect the residual plasma in the extracorporeal circulation and use it as the substitute fluid in the next performance. We can spare about 300ml of substitute fluid in every plasmapheresis by using this method.
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H. OHE, M. WATANABE, K. ISONO, H. TANAKA, H. KANAUCHI, K. HARA, T. TAK ...
1985 Volume 14 Issue 1 Pages
472-475
Published: February 15, 1985
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We studied a new autologous plasma exchange system with the chemical precipitation method. When we use a mixture of sodium chloride and glycine as the precipitant, globulin fraction precipitated with higher selectivity than albumin. The obtained precipitate was separated by filtration, and precipitant was removed by dialysis, respectively. In vitro test of normal human plasma, the removal rate of Alb, IgG, IgA and IgM were 28.9%, 88.9%, 68.7% and 61.8%.
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H. KANIYU, H. SUZUKI, T. SHIBATA, H. NIHEI, N. MIMURA
1985 Volume 14 Issue 1 Pages
476-478
Published: February 15, 1985
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It is well known that, the solubility of the protein to water depends on the density of the electrolytes that coexist in the solution, and as the density of the electrolytes increases, the solubility of the protein to the water decreases. Based on this theory, we tried to separate the plasma protein that we got by plasmapheresis into the fractions of γ globulin and albumin. We used the solution of highdensity sodium chloride for this purpose. When we used the nearly saturated solution of sodium chloride (32%), and when it's pH was 4.5, we could separate the most amount of γ globulin, especially IgG fraction from the plasma protein.
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A. SAITO, H. OGAWA, T. TAKAGI, T.G. CHUNG, M. MATSUMOTO
1985 Volume 14 Issue 1 Pages
479-482
Published: February 15, 1985
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Supernatant of plasma of which globulin was precipitated by mixing with NaCl and glycine in a bag was dialyzed and then used as regenerated plasma of plasmapheresis. This therapy called salt-amino acid-precipitation (SAP) plasmapheresis was applied for 3 rheumatoid arthritis patients and a SLE patient. 2.5 liters of plasma was exchanged once in a week and 5 to 12 times of the therapy was conducted in each case. Sieving coefficient of IgG was 0.28 and that of albumin was 0.74. No albumin was supplemented for more than 2-month SAP plasmapheresis. Reduction rate of immune complex in serum was 54.3±10.74%. A decrease of urinary protein was observed in the SLE patient. Improvement was also seen in two of the three rheumatoid arthritis patients.
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[in Japanese]
1985 Volume 14 Issue 1 Pages
483
Published: February 15, 1985
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H. SAITOH, T. SHIBAMOTO
1985 Volume 14 Issue 1 Pages
484-486
Published: February 15, 1985
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The effect of plasma separation on with membrane type plasma separator on the red blood corpuscle (RBC) was evaluated by the changes of hemoglob in absorbance of the filtrate.
The effect of transmembrane pressure (TMP) was also evaluated. The membranes of the separator investigated were cellulose diacetate (CA) and polyvinylalcohol (PVA). There was no difference between the inlet and the outlet of the separator in the hemoglobin concentrations, both in CA and PVA. In case of CA, however, hemoglobin absorbance was slightly increased to the increase of TMP, indicating the destruction of RBC. This change was not identified in PVA.
The hemolysis associated with plasma separation is sometimes difficult to detect, especially when it is with a small amount. The application of hemolysis detection by hemoglobin absorbance makes it possible in the early phase of its occurance.
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H. SAKURAI, T. KANAMORI, M. OSHIMA, K. OZAWA, S. TAKESAWA, K. SAKAI, J ...
1985 Volume 14 Issue 1 Pages
487-490
Published: February 15, 1985
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Temperature and total protein (TP) concentration are important variables affecting plasma viscosity, with viscosity depending on temperature in accordance with the Andrade equation and on TP concentration in accordance with Oshima's equation. Combination of these two equations provides an empirical equation correlating normal plasma viscosity to temperature and TP concentration. Despite the small difference in TP between HD patients and normal subjects, the HD patients show higher values of plasma viscosity and activation energy. It was found that the higher the globulin and fibrinogen concentration for HD patients by CA plasmaelectrophoresis, the higher the plasma viscosity and activation energy.
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T. KANAMORI, S. TAKESAWA, K. SAKAI, M. MINESHIMA, K. OTA
1985 Volume 14 Issue 1 Pages
491-494
Published: February 15, 1985
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Measurement of plasma and blood viscosity is valid especially for the hyperviscosity syndrome, which in one of the adaptative diseases to plasmapheresis. V-factor and I. V. F. with no effect of the variation in blood composistion were found in our previous papers to be extremely valuable variables in elucidating abnormal viscosity in HD patients. The decrease in viscosity during plasmapheresis occurs simultaneously with the decrease in Hct and Tp. Testing clinical treatment by plasmapheresis using V-factor and I. V. F. showed that in some cases, improvement did not occur. Both plasma and blood viscosity decreased with increases in the albumin fraction in total protein. It was found that V-factor and I. V. F. were also useful in evaluating plasmapheresis.
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Takayuki TSUJI, Tatsuo TOGAWA, Youji IMAI, Akihiko WATANABE, Hajime KU ...
1985 Volume 14 Issue 1 Pages
495-498
Published: February 15, 1985
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New viscometer based on tube viscometey was developed by using micropores of a polyvinyl alcohol (PVA) hollow fiber for plasma separates. After debubbling by alcohol infusion, blind-ended PVA fiber (ID: 200 micron, pore size: 0.2 micron) was infused at varing rates of constant flow of water which viscosity is known at the temperature. When water was oozed through micropores, the pressure (P)-flow (F) relationships showed good linearity. The coefficient (B) of the regression line (P=A+BF) at the known viscosity (V) decided a constant (K) characteristic of the tube resistance. The viscosities (V) of glucose solutions (5, 10, 20, 50%) obtained by this method (V=B/K) were equal to those measured simultaneously by a cone-plate viscometer.
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K. TAKEDA, H. YAMAUCHI, M. SUNAMURA, K. MIYAGAWA, Y. ASANUMA, S. MATSU ...
1985 Volume 14 Issue 1 Pages
499-502
Published: February 15, 1985
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Effects of plasma exchange on acute pancreatitis were investigated experimentally and clinically. Serum amylase, lipase, phospholipase A
2 levels decreased about 50% by 1600ml of plasma exchange in experimental acute pancreatitis. For 2-patients with acute pancreatitis, plasma exchange was performed. Serum pancreatic enzymes such as amylase, lipase, trypsin, elastase 1 decreased remarkably by 3000 or 4000ml of plasma exchange. Plasma exchange was effective for rapid removal of serum pancreatic enzymes in acute pancreatitis.
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T. TAKAHAMA, T. YOSHITAKE, K. YAGYU, K. WAKE, M. KAWAUCHI, F. MIYAWAKI ...
1985 Volume 14 Issue 1 Pages
503-506
Published: February 15, 1985
Released on J-STAGE: October 07, 2011
JOURNAL
FREE ACCESS
Preoperative plasma exchange decreased Ach R-Ab level of myasthenia gravis patients and enabled them very early weaning from respirator with ease.
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Y. UEDA, M. UEDA, N. YAMADA, Y. IKEDA, Y. YAGIRI, K. TAKAKURA, H. NISH ...
1985 Volume 14 Issue 1 Pages
507-510
Published: February 15, 1985
Released on J-STAGE: October 07, 2011
JOURNAL
FREE ACCESS
Plasmapheresis has recently evaluated as an alternative to intrauterine transfusion for the management of severe alloimmunized pregnancy. We have experienced five successful cases of severe rhesus alloimmunized pregnancy, by performing plasmaphereses. Two cases of which were successive pregnanices of the same woman in 1982 and 1983 We treated these two cases by performing plasmaphereses applying selective antibody adsorption using D (+) red blood cells. In our procedure, about 3 liters or 4 liters of maternal plasma was remored by an IBM 2997 blood cell separator. Then a mixture of one unit (In Japan, One unit is 200ml as fresh blood) of leucoute poor packed red cell sediment, and 350ml or 250ml of maternal plasma was incubated in an water 6ath of 37°C for 5 minutes. The incubation ratio of red cells and maternal plasma was 1:3 or 1:2. After centrcfugation and careful elimination of incubated red cells by Haemonetics Model The refined plasma was frozen for use as the replacement fluid in the next exchange. We perfomed 34 plasma phereses in her fourth pregnancy. The fact that we were able to perform frequent plasmapheresis safely for both the mother and infant underscores the efficacy of our procedure.
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[in Japanese]
1985 Volume 14 Issue 1 Pages
511
Published: February 15, 1985
Released on J-STAGE: October 07, 2011
JOURNAL
FREE ACCESS
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[in Japanese], [in Japanese]
1985 Volume 14 Issue 1 Pages
512-513
Published: February 15, 1985
Released on J-STAGE: October 07, 2011
JOURNAL
FREE ACCESS
