The author has tried complement fixation reaction on 958 leprosy serum specimens with Kephalin as an antigen. The positive rate was 49.1%. The Wassermann test has showed 21.4% positive reaction out of 1789 leprosy serum specimens.

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The Mycobacterium leprae murium which had been inoculated was found active and alive for amonth in a dog's body, 3 months in a cat, 2 months in a canary, a parrot, a budgerigerigar and each in the inoculated region or Iymphatic glands.

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In Java, . V. A. Calotinoid and V. C. found in cutaneous leprosy patients' blood were recognized slightly diminished in number. In nervous leprosy their numbers showed little change from healthy people. Judging from this survey, it seems that lack of vitamin is rather caused by leprous abnormality or developed symptoms than it is traced up to be cause of the disease.

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ABO type test was given to 469 lepers, and S type to 406 without finding any specific relation between abnormality types and constitution. Survey on 48 groups of lepers among kinship revealed no specific relationship to the disease.

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The following Mommsen's Puffer solution medium proved very favorable for staining the acid fast bacterium in tissues. Three per cent carboled 0.1% fuchsin is added to the puffer solution (1/10 N causatic soda solution 21.6cc:+1/10 Nice acetic acid 27cc+ distilled water 10L.) This solution, 30-60°C, is meant to stain for 10 minutes.

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Vitamin Kovarious concentration was poured into test tubes of Mycobacterium leprae murium, or was injected into white rats previously inoculated with Mycobacterium without finding any recognisable effect. However, in both cases it was found out that vitamin K enabled in a small degree Mycobacterium Ieprae murium and lepromas grow less actively than otherwise.

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Eighteen lepers, 72 healthy people, and 10 syphilitic and other dermatological patientswere examined with the No. 920 strain or pathogenic acid-fast bacillus culture medium filtered fluid. The intracutaneous reaction thus acquired was investigated in relation to the tuberculin test and the early Mitsuda reaction.
The result of the experimentation shows that the No. 920 strain filtered liquid intracutareous reaction reveals reddening and infiltration in 24-43 hours. When these symptoms have disappeared any latter reaction such as the Mitsuda test never appears. The early Mitsuda reaction, the No. 920 strain filtered liquid reaction, and the tuberculin reaction correspond to one another in 40-70 percent. However it can be ascertained that these three are activated by different factors.

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