Relationship between the reaction to Dharmendra's antigen and tuberculin reaction had been investigated in peoples of some towns and villages. In these studies, Dharmendra's antigen was injected on the skin of one side forearm and tuberculin was tested on the other forearm at the same time, and the relationship between both reactions to Dharmendra's antigen and tuberculin was different each other among the peoples examined in these towns and villages. The causes that could influence upon the positive rates to both reactions were analysed, and the relationship between both reactions to Dharmendra's antigen and tuberculin in tuberculosis pa-tients was compared with that reactions in BCG vaccinated peoples. As this results, the positive rate to Dharmendra's antigen in the tuberculin positive reactors among BCG-vaccinated peoples was about twice as that rate among tuberculosis patients.
These results show that the positive conversion of the reaction to Dharmendra's antigen was influenced at first by BCG-vaccination and secondarly by the infection of tuberculosis, but could not be influenced so many by the prevalence of leprosy in this report. And then, the difference of the relationship between both reactions to Dharmendra's antigen and tuberculin found in some towns and villages would be due to the diffusion grade of BCG-vaccination more than to the infection of tuberculosis. It was confirmed that M. leprae contained larger amount of common antigen in BCG than in M. tuberculosis hominis from these results. Therefore, a prophylactic significance of BCG against leprosy was considered.

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Numerous reports have already been published on the ABO blood type in leprosy patients and it has been stated that there is no significant difference in distri-bution compared to the Japanese in general.
The blood type in Japanese patients in the Oshima seishoen was examined. The percentage was determined according to the X²-method and the results compared to the findings obtained by the same method in 1932.

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There is no doubt that many attempts to transmit human leprosy to rabbits have been made by intratesticular inoculation. We found that the epididymis was often intensely attacked when atypical mycobacteria were inoculated into the testicle of rabbits. From this fact, it was thought, that inoculation with human leprous material into the epididymis of rabbits, might serve as a suitable site for the inoculation. Accordingly, the investigation was carried out with the object of ascertaining if the rabbits were susceptible to human leprosy when inoculated into the epididymis of the rabbits. After repeated tests its showed that rabbits were not susceptible to human leprosy after the third generation.
At the same time the same material was also inoculated into the testicle and extratesticle of mice. In this report the results of the experiments injected into the mice are stated. The human leprous material (LL2) was injected into the testis of mice. One of the ten mice lived for one year and more, but, 14 months after the inoculation, this mouse died showing marked evidence of infection; in direct smears and sections of liver, spleen and lung, abundant acid-fast bacilli and globi were found. The acid-fast bacilli were transmitted from mouse to mouse and all cultures proved sterile. It is inferred that the lesion may be mice leprosy.
There is convincing evidence of an immunologic relationship between M. Leprae and M, tuberculosis. According to the method of preparing Mitsuda antigen, antigen of LL2 in mouse and murine leprosy were made. And then 0.1ml, of the "Old Tuberculin", Mitsuda antigen, LL2 in mouse and murine leprosy (Hawaii) antigen, were injected at the two sites intracutaneously in two parallel rows along the backs of guinea pigs sensitized with the tubercle bacillus. After that the animals were observed for four weeks, and the skin reactions for each group were averaged. From the results ; it is inferred that both LL2 in mouse and murine leprosy are identical.

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The results of the 1st and 2nd report are summarized as follows. A mycobacterium was isolated from mice inoculated with human leprous material into the testis and extra-testis as shown in the first report. This bacillus is transmissible from mouse to mouse and unable to be cultivated on artificial culture media. Besides, LL2 was difficult to differentiate morphologically from murine leprous Hawaii. Pattern of the skin test on guinea pigs sensitized with the attenuated tubercle bacillus showed that antigen of the LL2 in mouse and antigen of the murine leprosy Hawaii are iden-tical. These two patterns are different from the Mitsuda antigen. But the antigen pattern of LL2 and antigen pattern of murine loeprsy Hawaii indicated different reactions by means of gel diffusion test. Therefore, it is believed that LL2 and murine leprosy Hawaii are of a different strain.

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Detailed observation of the distribution of acid-fast bacilli in the various parts of the healthy mouse is important as a measure for obtaining knowledge on the route of invasion of the organism into the animal and by the summation of the findings in numerous investigations, it may become possible to clarify the mechanism of infection of the murine leprosy bacillus under natural conditions.
In relation to the above, the distributuion of acid-fast bacilli following administration of DDS and INH and bacillary distribution in animals other than the mouse were also examined.
The acid-fast bacilli were collected by the Dharmendra's method using chloroform and ether. The skin was depilated and washed, the organs were cleansed well to eliminate all bacteria which may be adhering to the surface. The bacteria count was made by the formula given below.
×quantity of bacterial suspension No. of organisms per 1 loop quantity per loop (0.002 ml) x=No. of samples
The specimens were prepared by smearing one loopful of bacterial suspension on a glass slide, covering with phenol gelatin water and staining with Ziehl-Neelsen stain.
The results are shown in the table. As may be seen, a considerable number of bacilli was present in the skin and foot pad of the mouse and in the organs, a somewhat larger number was found in those organs connected with the outside. There was no pronounced differnce according to the site of the skin. No difference in bacterial count was found between the 6-months DDS and INH treated mice and the untreated control.
It is notewarthy that acid-fast bacilli were isolated from the foot pad of the guinea pig, rabbit and cat and from the skin nerve of the monkey. Studies must be carried out in the properties of these organisms in the future

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