1974年8月,国立多摩研究所を通じてE. E. Storrs博士から分与されたアルマジロのらい腫-L154(456)-から菌液を調製し,水酸化ナトリウム•塩酸の前処理を行なったのち,20匹のマウスの左後肢足底内に接種するとともにMFP細胞による細胞培養をこころみた。マウス足底内ではこの抗酸菌はらい菌と全く同様の発育パターンを示し,その足底からの菌は培養陰性で,ピリジン処理によって抗酸性は失ったが,そのグラム染色性は保たれていた。従って,このアルマジロらい腫がらい菌の増殖によって形成されたものであることには疑問の余地はない。
他方,細胞培養において,60日間の初代培養では菌数は約3.3倍に増加し,細胞ならびに培養液に外観上何らの異常もみとめられなかったが,これをトリプシン処理して第2代培養を行なったところ,培養液が次第に混濁し,その塗抹標本中に無数の抗酸菌を証明した。その一部を小川培地に移植すると,3週培養後に遅発育性で暗発色性の抗酸菌が分離された。細胞培養分離菌の性状をRunyon II暗発色遅発育抗酸菌の代表菌株であるM. scroflaceumおよびM. gordonaeのそれと比較検討した結果,分離菌はTween 80水解陰性かつ5μg/mlエタンブトール耐性で,その性状はその他の成績とともにM. scrofulaceumのそれに一致した。
従って,供試したアルマジロらい腫は使用前すでにM. scrofulaceumに汚染していたといえる。しかし,初代細胞培養における菌数増加度が小さく,かつ培養所見に異常がみられず,第2代で急に培養液中に多量の抗酸菌をみとめたことは,本菌の汚染が微量で,初代培養では細胞内に限局して発育し,それが第2代に一部細胞外に遊離したために著明な増殖を来したものと考えられる。
らい菌の培養実験にアルマジロらい腫を用いる場合,この点に十分の注意をはらう必要があろう。

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Surgical reconstruction in the paralyzed hand
1) Low ulnar nerve palsy: The extensor digiti quinti proprius muscle is transferred to combat the loss of PIP extension of the small and ring fingers.
2) Clawed fingers due to low median and ulnar nerve paralysis are corrected by the extensor many tailed graft or extensor to flexor many tailed graft of Brand, or by a modification of Bunnell's method. For the reconstruction of opposition of the thumb, Riordan's, Tsuge's, or Brand's method are used. When these methods cannot be used, the extensor carpi radialis longus, the pronator teres and the extensor digiti quinti proprius may be used as the motor. The flexion deformity of the IP joint or the swan neck deformity of the thumb are corrected by splitting the flexor pollicis longus tendon and attaching one end to the volar side of the proximal phalanx or turning it dorsally to suture with the extensor pollicis longus tendon. Occasionally an IP arthrodesis is performed. The pulley is usually made as a loop on the periphery of the flexor carpi ulnaris tendon. The abductor digiti quinti may also be utilized as the pulley.
3) High median and ulnar nerve paralysis.
a) The Brachioradialis is transferred to the flexor pollicis longus and the extensor carpi radialis longus to the flexors digitorum profundus of the index through little fingers.
b) The clawed fingers are corrected by tenodesis of the flexor digitorun sublimis of the middle finger.
c) For reconstruction of opposition of the thumb, the extensor digiti quinti proprius is routed around to the ulnar side of the wrist and sutured to the extensor pollicis longus tendon. In cases in which only the flexor pollicis longus is paralyzed, the Brachioradialis or extensor carpi radialis longus may be transferred after correction of the clawed fingers and reconstruction of opposition.
4) High palsy of the radial nerve plus low palsy of the madian and ulnar nerve: First operation: The pronator teres is transferred to the extensor carpi radialis longus and brevis tendon. The flexor carpi radialis tendon is brought to the dorsum through the interosseous space, and sutured to the extensor pollicis longus and extensor digitorum communis tendon. Second operation: The clawed fingers are corrected by a modifica- tion of Bunnell's method. Third operation: The thumb opposition is reconstructed. The Riordan's method may also be used for the simple high radial nerve palsy.
5) In a simple low paralysis of the radial nerve, the extensor carpi radialis longus is transferred to the extensor pollicis longus and the extensor digitorum communis tendon. In the simple paralysis of the extensor pollicis longus, the extensor digiti quinti proprius is transferred. Restoration of opposition of the thumb is performed as necessary as a second operation.
6) High median, ulnar and radial nerve paralysis : Flexor tenodesis is performed utilizing the flexor tendons of all fingers.
7) Occasionally, intrinsic muscles are spared, but all flexors of the fingers are paralyzed. For this type, the brachioradial is transferred to the flexor pollicis longus and the extensor carpi radialis longus to the flexor digitorum prof undus. In addition to these basic tendon transfers, arthrodesis and tenodesis may be used as necessary.

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