50 psychiatric cases were observed at Aisei-en during the period April 1957-April 1958. They consisted of : a) Schizophrenia (12 cases) ; manic-depressive psychosis (1). b) Epilepsy (2) ; dementia senilis (6) ; disorder due to cerebral hemorrhage (1) ; general paresis (1). c) Mental deficiency (3) ; psychopathic personality (13) ; neurosis (11). Details on the forms of the above cases were given together with 3 case histories where leprosy and its sequelae were involved as psychogenic factors. The nature of the psy-chiatric cases was discussed with the following conclusions : 1) The incidence of functional and organic psychoses was 0.75% and 0.58% respectively;therefore no great difference was found from the rates in the total Japanese population. 2) There was to be found no form of psychosis peculiar to leprosy. 3) No special relations between the forms of leprosy and the psychoses were found. 4) Leprosy and its consequences were to be recognized as psychogenic inducement in the outbreak of 2 casof schizophrenia, as causes of a schizophrenic reaction in 1 psycho-pathic personality and as causative factors of several anxiety neuroses. 5) The rate of suicide at Aisei-en was 58 per 100, 000, which shows no great difference from that in the total Japanese population.

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In order to learn the immunological relationship between tuberculosis and leprosis, serum samples of 200 leprous patients who were proved clinically and bacteriologically to have no lesion of tuberculosis, were tested by the Middlebrook-Dubos' passive hemagglutination and the hemolytic test after Sindo-Meddlebrook's forumula using the tuberculin fraction Fr. II as the antigen.
In the passive hemagglutination, as a whole, positive rate was 80%, and tde mean value of end titres was 1: 33.2. The positive rates and their mean values in both cases of lepro-matous and tuberculoid leprosies were 85%, 67.7% and 1:39.7, 1:17.6 respectivery.
In the hemolytic test, on the whole, positive rate was 81.0% and it's mean value of end titres was 1:85.8. Further, the positive rate and mean value of end titres in the sases with lepromatous leprosy was 82.9% and 1: 93.0 respectively, and 76.1% and 1: 65.5 in cases with tuberculoid leprosy.
Thus, the positive rate and the mean value of end titres in both seroogic testsin cases with lepromatous leprosy were higher than those in cases with tuberculoid leprosy.
The averaged pattern of the two-dimensional hemolytic test was less steeper than those in tuberculosis.
In the present investigation of leprosy, no correlation between results of both the tube-rculin skin reaction and the hemolytic test was obsered. This fact is quite different from the picture in healthy adults in Japan.
Tee positive rate of hemolytic test of tuberculin test-negative ones was in some degree lower than that of tuberculin test-positive ones, while the mean value of hemolytic titre of the former was higher than that of the latter.

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1) 癩菌の菌数計算法として大腸菌との比例計算による方法を試み,それを使つて種々臓器中の人癩および鼠癩の菌数を算定した。 2) マウスおよびラッテの鼠癩皮下結節は1g当り200億ないし400億の菌を含んでおり,マウス結節はラッテ結節より多く菌を含んでいた。 3) 人癩結節1g中には,20億ないし60億の菌が認められた。 4) 国立らい研究所において1957年度および1958年度に製造されたレプロミンはほぼ同数の菌を含んでおり,1cc当り約2億の菌数である。

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1) 算定された一定菌数の鼠癩菌を鼻孔滴下,睾丸内注入,脳内注射,静脈内注射,開腹後脾臓内注射の5経路によりマウスに接種し,以後逐日的にその臓器内で増殖する鼠癩菌の菌数を数えその発育曲線を求めて観察した. 2) 経時的に証明せられた菌数の増加状況から見て,菌数の倍増するに要する期間を一世代として感染方法の異なる場合の世代期間を比較した。 3) 以上の菌発育曲線で見ると,5経路の中,睾丸内接種による際の発育が一番速かであり,体内各臓器えの分布も早期より認められ,その世代時間は7日から10日に相当した。これに次ぐものは開腹後脾臓内接種の10日ないし15日であつた。脳内接種後の脳内での発育と静脈内接種後の脾臓内ての発育は大体同じ速度で,2週ないし3週の世代時間であつた。 4) 脾臓内に直接に菌を接種した場合と間接に接種した場合における菌の発育速度には著明の差を認めなかつたが,幾分前者の方が早い傾向にあつた。 5) 鼻孔に総量として34万匹の菌液を滴下した群では,接種後6ケ月間は著者の測定条件では何れの臓器からも菌を見出し得なかつたが,7ケ月を過ぎたマウスでは19匹中18匹から菌が検出された。ただしこの菌のマウス体内への撒布は必ずしも月数に比例してはいなかつた。

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マウス睾丸内に人癩菌ほか数種の抗酸性菌の加熱菌および無加熱菌を接種し,日を追つてその菌数を追求し,つぎの如き結果を得た。 1) 無加熱人癩菌では接種後300日聞にわたり殆んど菌数に増減を見なかつたが,加熱人癩菌では徐々に菌数が減少した。 2) BCG,モルIII,スメグマの各無加熱菌では接種後70日ないし90日位までは復元培養が可能であつたが,その菌数は日を追つて減少していつた。 3) BCG,モルIII,スメグマの各加熱菌の菌数も日を追つて減少したが,モルIII菌,スメグマ菌ではその減少の度合がそれらの無加熱菌の減少の度合よりも緩徐であるように思われた。 4) 加熱鼠癩菌は無加熱人癩菌と同様の態度をとり,接種後100日余にわたりその菌数を殆んど減少しなかつた。

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The basis for the selection of purified cephalin for use as the antigen is discussed. The difference in reaction compared to the preparation purified by the J. Folch method is presented.

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Studies were conducted using a combination of ox brain cephalin faction, 0.2% cardio-lipin and 1% cholesterol supplemented with sphingomyelin extracted from ox brain. A reaction was noted at a maximum of 256-fold dilution in the nodular form, 16-fold in the neural form and 256-fold in the macular form. Addition of lecithin resulted in a reduction in sensitivity of the complement fixation reaction in leprosy and a greater reaction in syphilis. Furthermore, a reaction was noted at 16-fold dilution with pregnancy serum.

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The selection of the mouse strain adequate for murine leprosy experiments has been investigated in the series of previous papers. Through these experiments, it was found that C 57 BL and C 3 H strains were suited for the purpose. Since these two strains can not be easily maintained in a satisfactory condition, the author conducted the following experiments to find the mice which have similar properties to C 57BL and C 3 H and more manageable than these inbred strains in breeding and feeding.
To meet these requirements, the author intended to establish a first generation hybrid (F1) by crossing a male of a susceptible strain with a female of a manageable strain. Therefore, the selection of the parental strains for mating was carried out at first.
The susceptibility to subcutaneous inoculation with murine leprosy bacilli of mouse strains (C 57 BL/6, A 1, NC, dba, BALB/C, SM, C 3 H/He and C 3 H) was examined to select the male parental strain. As a result of this experiment, C 57 BL/6 and C 3 H strins were adequate for the purpose.
As the possible female parental strains, which are easier to breed, ddY and ddD were selected. In susceptibility to murine leprosy bacilli, ddY strain was found to be superior to ddD strain.

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