At first, T and B lymphocytic subpopulations were examined for different surface markers on 5 cases of human fetal lymphoid tissues at 14 to 22 weeks of gestation (CH 15cm-29cm), and on 25 cases of human embryonic and fetal livers at 5 to 22 weeks of gestation (CR 8mm-CH 29cm).
In the second study, hepatic haematopoiesis were studied with electronmicroscope on 25 cases of livers ranging from 4 to 22 weeks of gestation. Fetal age were caluculated by fetilization age and by crown to rump or heel length. The results obtained were as followings; 1) More than 70 per cent of lymphocytes in the bone marrow and liver were null cells, in contrast to most of lymphocytes in thymus and lymph nodes were E and/or EAC rosette forming cells.2) In the liver, EAC rosette forming cells were first appeared at 6 weeks, then E rosette forming cells at 7 weeks, smIgM-cells and smIgD-cells at 10 weeks, smIgG-cells at 11 weeks and at last, smIgA-cells at 22 weeks of gestation.3) After 5 weeks of gestation, several types of undifferentiated mononuclear cells and mesenchymal cells were mainly seen in the extrasinusoidal spaces.4) The erythroid progenitor cells were first detected at 5 weeks of gestation only in the extrasinusoidal spaces and neutrophils in both the sinusoid and the extrasinusoidal spaces at the same time. After 6 weeks of gestation, Myeloblasts, lymphoid cells and megakaryoblasts were seen in both spaces and monoblasts only in the extrasinusoidal spaces.5) In hepatic erythropoiesis, several findings such as breakdown of nuclear membrane with myelinization, cytoplasmic invagination into the nucleus, nuclear extrusion to cytoplasma, binuclearity, nuclear cleft, nuclear bleb, and frequent mitotic figures were observed. These findings were similar to the cases of congenital dyserythropoiesis.
Multipotential stem cells could n ot be identified morphologically, but several undifferentiated mononuclear cells, which were presumably stem cells, could be found only at very early stage of hepatic haematopoiesis mainly in the extrasinusoidal spaces or in the hepatic cord which was directly connected with sinusoid. We could not decide whether undifferentiated mononuclear cells derived from yolk sac or from mesenchymal cells originated from septum transversum. However it is likely that the undifferentiated mononuclear cells (stem cells)come from both yolk sac and hepatic mesenchymal cells on the basis of cell appearance and their distributing course.

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[in Japanese]

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Experiments with, rabbits were made of M wave and H wave in reference to the effects of wearing contact lenses.
1) On the p eak time of M wave and H wave in the m. gastrocnemius induced by the tibial nerve stimulation, wearing contact lenses showed almost no effects.
2) The amplitude of M wave was not changed significantly, w hile that of H wave was increased by wearing contact lenses.
3) It was assumed that the increase in amplitude of H wave was due to facilitation of the monosynaptic reflex following inhibition of the reticular formation.

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With the use of an average response computer (MEDIAC) a study was made of the effects of wearing contact lenses on the late components of afferent average evoked potentials. The results obtained were as follows.
1) The late componen ts such as N6, N7 of the average evoked potentials in the cerebral cortex due to the sciatic nerve stimulation were inhibited by wearing contact lenses.
2) The late component of HN6 of the average evoked potentials in the hipp ocampus due to the sciatic nerve stimulation were inhibited (when it was induced from P3) or facilitated (when it was induced from P5) by wearing contact lenses.

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With the use of an integrator (Nihon Kohden RFJ- 5) a study was made of the effects of wearing contact lenses on the evoked muscular discharges elicited by stimulation of the cerebral cortex and the hippocampus in rabbits. The results obtained were as follows.
1) The evoked muscular discharges in the fore and hind limbs following 10 0Hz stimulation of the cerebral cortex were inhibited by wearing contact lenses.
2) The evokd muscular discharges following 100Hz st imulation of the hippocampus were inhibited in some cases and facilitated in others by wearing contact lenses.
3) It was assumed that the activity of the pyramidal tract was i nhibited and the activity of the extrapyramidal tract was inhibited or facilitated by wearing contact lenses.

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An electrophysiological study was made of the effects of wearing contact lens on the MV (microvibration) with the use of an average response computer (ATAC-201).
1) MV response induced by photic stimulation comprises of MV₁, MV₂, MV₃, MV₄ and MV₅ components. These components showed a facilitation in the form of an increase in their amplitude following wearing contact lens.
2) Discussion was made of a poss ible increase in excitation in the reticular formation and the hippocampus regarding the facilitated MV thus induced.

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With the use of ATAC-350 (Nihon Kohden) a study was made of the effects of wearing contact lenses on the unit discharges of the reticular formation induced by photic stimulation in rabbits.
1) By use of an interval histogram, it was demonstrated that wearing contact lenses led to a marked increase in the frequency of unit discharges in the midbrain reticular formation induced by photic stimulation.
2) In the pulse count histogram, an increase in the frequency of the unit discharges of 90msec latency induced by photic stimulation was observed by wearing contact lenses.
3) It was assumed that the increase of the unit discharges was due to an increase of activity of the reticular formation which was elicited by the pain following wearing contact lenses.

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The status of the unexpected antibodies detected in blood donors or recipients and in the newborns with haemolytic diseases, using physiological saline test, high protein test, bromelin test and anti-globulin test in our blood transfusion unit were reported.
1.226 samples out of 12,875 samples were positive for unexpected antibodies. Anti-D, -E, -Le^a, -Le^b and -P₁ antibody were predominant among the antibodies.
2. It should be noteworthy that about 40% of anti-Le^a or Le^b antibodies, which may cause hemolytic diseases, were detectable only by anti-globulin test.
3. Among warm-type auto-antibodies showed Rh specific ity (anti-C, anti-E, anti-D antibody).
4. Among 160 samples from incompatible pregnancies,21 gave positive results: i. e.15concerning Rh system, and 6 ABO system.
5. Exchange transfusion were car ried out in 10 cases due to hemolysis caused by blood type incompatibilities: The antibodies detected were 1 anti-D,1 anti-D+ E,3 anti-E,2 anti-E-F c, as well as 1 anti-A and 2 anti-B. These results suggest the importance of E antibody, not only D antibody, for transfusion and pregnancy.

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For the purpose of approaching the actualities of epileptiform convulsions which appear in the patients with various psychotic disorders during their drug treatment, the author has studied 70 cases which were collected in six affiliate hospitals with a special reference to back ground psychotic disorders, preceding medications, convulsive predisposition, electroencephalographical features, course and treatment following the first convulsive incidence.
As to the back ground psychoses, it is evident that convuls ions are liable to appear in some phasic or periodic type of disorders such as atypical psychoses, catatonic type of schizophrenia, and so on. Family history of psychotic and convulsive disorders as personal history of childhood convulsion and electroshock therapy were frequently found in the studied cases. Furthermore, those who had some evidence of convulsive predisposition have presented an earlier onset of clinical attack and a higher tendeney of its reccurence than the others.
Among psychotropic drugs, phenothiazines and tricyclic antid epressants seems to be more risky and butylophenones to be less risky as far as convulsive attack is concerned. The clinical course and outcome of convulsive incidence are quite similar to that of childhood convulsions, of which the majority have the attack only once for life.
As the results of this study, the author has been c onvinced that the above mentioned convulsive incidences are some kind of occasional convulsive seizure (Gelegenheitskrampf) induced by the administration of potent paychotropic drugs.

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It is widely accepted that there are no specific abnormalities but some deviations within normal limit in electroencephalogram as far as endogenous depression is concerned. Certain traits in electroencephalographic findings appear to be related to those of clinical features in patient with depressive psychosis. However, any particular correlation has never been confirmed between electroencephalographic finding and clinical symptom. Therefore, it will be much more reasonable to cross-examine electroencephalographic alterations and clinical evolutions under the influences of thymoleptic drug treatment. In the present communication, the author has studied successive electroencephalographic records of twenty patients with endogenous depression using quantitative analytic method in reference to their psychopathological evaluations before and during the treatments with tricyclic antidepressants; imipramine and amitriptyline.
For the present study, twenty patients with endogenous depression, who had not been medicated for more than one month or who had never been treated before, were selected and carefully investigated clinically as well as electroencephalographically. They were divided into two groups based on their clinical types; inhibitted, agitated and mixed. The group consists of inhibitted and mixed types was given imipramine 25 mg three times daily while the other of agitated type received amitriptyline 25 mg three times daily at fixed dose at least for the first week. Daily dose was decided at doctor's choice thereafter and the patients were treated with solely either one of the two drugs for one month. Electroencephalographic recording was carried out before the first administration of the drug, three hours after the first administration, one week and one month after the treatoment and was analyzed later using CEEG methnd by Itil and Saito. Clinical evaluation was done using Extended Brief Psychiatric Rating Scale (EBPRS, KMU edition) before, one week and one month after the treatment. The results are as follows.

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