Electropharmacological studies have been done by many investigators in order to determine the site of action of anesthetics and analgesics in central nervous system. Cortical action potential i. e. reverberating response, recruiting response, local cortical response and also spinal action potential in afferent as well as efferent pathways were used in these experiments as indicator of activity of central nervous system. On the other hand, electromyographic experiments on reflex activtity of spinal cord with unanesthetized rabbits have in past few years been carried out in our laboratory and the influences of these drugs on nociceptively and proprioceptively induced reflex pattern of hind limb were investigated in reference to the central action of these drugs.
The results reported in the papers were picked up and discussed.
(1) In barbiturate anesthetized animal, morphine blocked splanchnic afferent synapses in spinal cord and depressed intracortical neurone activity. Conduction of repetitive impulses through N. ventralis posterolateralis of thalamus was suppressed by morphine as well as aminopyrine.
(2) The activity of ascending activating system was depressed by barbiturate and ether but aminopyrine might be probably stimulating on this system.
(3) Barbiturate depressed significantly nociceptively induced muscle-after-discharge in spinal and midbrain rabbit and proprioceptively induced muscle-discharge in spinal rabbit. It was suggestive that barbitutrate also affected on central motor regulatory system.
Morphine depressed these muscle-discharges in the same degree in spinal lebel, and in nociceptive muscle-discharges, the one originated in higher center was more susceptible to morphine than the others. Aminopyrine suppressed spinal interneuronce-interneurone synapses.
(4) It was discussed that morphine exerted the strong action at higher center as well as spinal cord and aminopyrine at spinal cord and that morphine or barbiturate had both direct and indirect action on the central nervous system.

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Many clinical reports have been made so far from various angles as to the relationship between tuberculosis and asthma. However, they do not seem to have reached any definite agrement.
The present study has been made, under M. Takino's suggestion, of 8 typical asthmatic cases complicated with pulmonary tuberculosis, 2 typical asthmatic cases of tuberculous origin and 5 atypical cases of asthma, i. e. asthmatic bronchitis. These cases have been observed from an angle of the mechanism of the occurrence of asthmatic attacks in reference to disorders of the autonomic nervous system concerning tuberculosis allergy. They have had a considerably long follow up. The results thus obtained are as follows.
1. Asthma, in view of its fundamental allergic constitution, does not seem to be a causative factor of pulmonary tuberculosis.
2. Constitutionally asthmatic patients may appear highly resistant against tuberculosis. But, once they are complicated with tuberculosis, an attack of asthma and various conditions accompanied thereby semm to hinder the localization of the lesion, giving an unfavorable influence upon pulmonary tuberculosis.
3. Contrary to infectious respiratory diseases of various kinds, pneumonia, whooping cough for instance, pulmonary tuberculosis generally does not seem to be a causative factor of typical asthma, even in those who come from the families with a positive history relative to allergic diseases or essential hypertension. Tuberculosis is rather considered to inhibit the occurrence of typical asthma.
4. Of course, typical asthma, though rarely, occurs in the tuberculous patients, only when a clinical course of the tuberculosis is very favorable. However, it seems that they come from the families with a positive history relative to essential hypertension or allergie diseases.
5. Asthma which is caused by pulmonary tuberculosis generally seems to take an aspect of atypical asthma with mucous symptoms, i. e., asthmatic bronchitis rather than typical asthma with an intensive bronchospasm.
6. Tuberculosis seems to influence asthma through specific changes of the parasympathetic nerve caused by tuberculosis allergy. An asthmatic attack of asthmatic patients complicated with tuberculosis is generally severe when tuberculous symptoms are improved, and is mild when the symptoms become worse, no matter whether the occurrence of asthma preceeds or follows that of tuberculosis.

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In order to make clear how Curschmann's spiral, especially its central fibre is produced in the respiratory tract, I have histologically and histochemically investigated not only the sputum of asthmatic patients, but also the trachea, the bronchus, their mucous, serous and mixed glands and their ducts in the autopsies of the patients died during asthmatic attacks.
The materials of the lungs were carefully fixed in 10% formalin solution, which was kept warm at 38°C. They were partly cut by means of celloidin-method and partly frozen method. For general observation of the lungs haematoxylin-eosin staining method, for that of mucus cresylechtviolett staining method, and for that of nuclear component nuclear staining method (Feuigen) was used.
The results were as follows:
1. According to my investigation, Curschmann's spiral is chiefly composed of mucus. Although their central fibres are chiefly composed of far thicker mucus than the other parts of the spiral, they contain cellular components, specialy nuclear components, derived from mucous cells, leucocytes, eosinophil cells and plasma cells, as well.
2. The fine central fibres are already produced in the mucous cells of mucous and mixed glands as well in tbeir ducts.
3. Some of thick central fibres in the lumen of respiratory tract is composed of the mixture of thick mucus and cellular components caused by the destruction and fusion of the above cells in that tract.
4. As soon as the fibres of various size enter into the lumen of the respiratory tract, they gather together and are twisted, because of the production of a whirl-pool of air in the lumen of the wall during asthmatic attacks. Thus, the thick central fibres are produced. However, I was not able to find anywhere that the central fibres are made of the mucus from the goblet cells. Therefore, whether or not these cells also takes part in production of central fibres, requires further minute investigalon.

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Studies on the function of reticuloendothelial system of animal were made through the comparison of the value of congo red index and phagocytic ratio of histiocytes in subcutaneous tissue to india ink, and blood picture of normal rabbits.
No exact paralleled reaction was found between the values obtained by the conga red method and phagocytic ratio of histiocytes of subcutaneous tissue and also through the blood picture, value of the mean number of nuclear lobes of multinuclear leucocytes, there seems to be no close relation between them.

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The Function of reticuloendothelial System of individual rabbits estimated by congo red method is fluctuating in a certain range under normal conditions.
The influence of multin which has been known as a unspecific immunantigen on the fluctuation of reticuloendothelial system was observed on each different rabbit.
The results obtained were as follows:
1) In case multin is intravenously injected on a rabbit, it some times increases the function of reticuloendothelial system. The increase is often accellated by the injection and falls down into normal range or below it in a short time, after which it some times rises or does not rise.
2) The effect of multin injection on reticuloendothelial system is no relation with the proper value of the physiological congo-red index of the animal. There seems to be some unknown cause of the fluctuation due to the multin injection.

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Experimental studies are made on the lysis of leucocytes of tuberculous patients due to tuberculin and also on the factor which has been introduced by Favour and others. The results obtained are as follows:
1) The factor of the lysis of leucocytes in vitro due to tuberculin is present in blood plasma of the patients.
2)The effective factor in the plasma is destroyed at 56°C,15 min., but it is preserved at 10°C for a week.
3) When serum is used in place of plasma, the lysis of leucocytes is not remarkable.

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