The Journal of Kansai Medical University

Electron microscopic studies of liver embryogenesis in humans I.

The parenchymal cells and the endothelial cells

Tadashi Sasauchi

1971 Volume 23 Issue 1 Pages 1-31
Published: March 20, 1971
Released on J-STAGE: February 19, 2013

DOIhttps://doi.org/10.5361/jkmu1956.23.1_1

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The livers of human embryos 8-32 weeks of age were studied electron microscopically. The liver cells showed marked differences in ultrastructure in various phases of development. The free polysomes decresed in number and the endoplasmic reticulum appear to undergo structural reorganization from vesicotubular to lamellar type around the third month. 'Simultaneously the smooth endoplasmic reticulum and hemosiderin granules appeared, and the glycogen granules increased markedly. These sturctural changes probably correspond to the start of the embryonic hematopoiesis and the biosynthesis of serum proteins in the liver.

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[title in Japanese]

1971 Volume 23 Issue 1 Pages e1-
Published: 1971
Released on J-STAGE: February 19, 2013

DOIhttps://doi.org/10.5361/jkmu1956.23.1_e1

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Electron microscopic studies of liver embryogenesis in humans II.

The hematopoietic cells.

Tadashi Sasauchi

1971 Volume 23 Issue 1 Pages 32-50
Published: March 20, 1971
Released on J-STAGE: February 19, 2013

DOIhttps://doi.org/10.5361/jkmu1956.23.1_32

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The ultrastructural changes of the erythropoietic cells in the fetal liver were studied electron microscopically, and these changas were tried to correlate with the cytochemicrl studies on erythrocytic development.
Incorporation into the eythropoietic cells of ferritin particles by rhopheocytosis (micropinocytosis)was observed in detail. The ultrastructure of the thrombopoietic cells was also clescribed. Inturelationships of the parenchymal -cells with mesenchymal hematopoietic cells were studied and discussed.

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Studies on blood cells of the silkworms, Bombyx mori I.

Light microscopic studies.

Takatoshi Yokota

1971 Volume 23 Issue 1 Pages 51-66
Published: March 20, 1971
Released on J-STAGE: February 19, 2013

DOIhttps://doi.org/10.5361/jkmu1956.23.1_51

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Blood cells of the silkworm, Bombyx mori, were samplied sucessively from the beginning of the third instar to the pupal stadium, and were oberved by a light microscope. The blood cells were classified to five kinds; proleucocytes, plasmatocytes, granular cells, spherule cells and oenocytoides, and their structural properties were described. Further, it was suggested that the granular cells are derived from the proleucocytes. The cell count ( or the number of cells in 1 mma ) of the silkworm showed a cyclic change; minimum or decreasing in the beginning of each instar, then increase rapidly, arriving at a maximum number during molting period (or, in the fifth instar, two days after the ripening). The granular cells and proleucocytes were the main components of the blood cells. In the early stage of each instar, the plasma cells were numerous, which, however, decreased gradually and in the molting stadium compensatory increase of the granular cells was observed.

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Studies on blood Cells of the silkworms, Bombyx mori II.

Electron microscopic studies

Takatoshi Yokota

1971 Volume 23 Issue 1 Pages 67-92
Published: March 20, 1971
Released on J-STAGE: February 19, 2013

DOIhttps://doi.org/10.5361/jkmu1956.23.1_67

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Ultrastructure of five kinds of blood cells of the silkworm, Bombyx mori, has been studied. Proleucocytes are primitive cells, rich in free polyrihosomes and containing a few rough endoplasmic reticulum. It is suggested that granular cells and spherule cells are differentiated from the proleucocytes. Ultrastructure of the plasmatocytes is quite similar to that of the proleucocytes. Granular cells contain well developed rough endoplasmic reticulum with dilated cisternal cavity, as well as a number of specific granules that are probably formed by the Golgi apparatus. The granular cells are active in phagocytosis and in secretion. Spherule cells usually contain several large spherical granules which are probably to be secreted out of the cells. Oenocytoides have a number of cytoplasmic free polyribosomes and inclusion bodies. There are two kinds of inclusion bodies; one is fibrous material composed of fine filaments and the other is round or oval bodies with homogeneous content. It is suggested that these inclusion bodies are proteineous materials synthesized by the free polyribosomes.

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[title in Japanese]

Junichi Ito

1971 Volume 23 Issue 1 Pages 93-101
Published: March 20, 1971
Released on J-STAGE: February 19, 2013

DOIhttps://doi.org/10.5361/jkmu1956.23.1_93

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[title in Japanese]

Junichi Ito

1971 Volume 23 Issue 1 Pages 102-106
Published: March 20, 1971
Released on J-STAGE: February 19, 2013

DOIhttps://doi.org/10.5361/jkmu1956.23.1_102

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[title in Japanese]

Junichi Ito

1971 Volume 23 Issue 1 Pages 106-111
Published: March 20, 1971
Released on J-STAGE: February 19, 2013

DOIhttps://doi.org/10.5361/jkmu1956.23.1_106

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[title in Japanese]

Junichi Ito

1971 Volume 23 Issue 1 Pages 112-114
Published: March 20, 1971
Released on J-STAGE: February 19, 2013

DOIhttps://doi.org/10.5361/jkmu1956.23.1_112

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The experimental study on the neurotoxic action of phthalodinitrile

Junichi Ito

1971 Volume 23 Issue 1 Pages 115-126
Published: March 20, 1971
Released on J-STAGE: February 19, 2013

DOIhttps://doi.org/10.5361/jkmu1956.23.1_115

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The toxic action of phthalodinitrile (abbr, P D N) on the nervous system was investigated by the electrophysiological method in rabbits.
1. The neuro-physiological investigation of the cramp attack caused by P D N and its neurotoxic action.
2. The effect of the repeated administration of P D N by the inplanted electrode method.
3. The effect of anti-convulsants on the cramp attack caused by P D N.
4. The patho-histological investigation of the toxic action of P D N.
5. The summary and consideration of the experimental investigations.
The results obtained are as follows:
1) Clonic cramp attacks of the whole body were always observed when over 25mg/kg of P D N was administered intraperitoneally and also sometimes when 10-20 mg/kg of P D N was administered.
The repeated administration of, a small dose caused cramp attack, and a single administration of the dose did not cause it.
2) An administration of P D N acted inhibitorily on the arousal reaction in the E E G caused by the electro-stimulus in the thalamus and midbrain reticulum formation an d acted acceleratively on the simultaneously evoked muscle discharges.
3) The seizures were evoked in the E E G of neo-and archi-cortex after the administration of P D N to the rabbits of cerveau isole, however the cramp attacks of the whole body were not observed.
Anti-convulsants, that is, Acetazolamide, Dichlorphenamide, etc, depressed the seizure waves in the E E G caused by P D N.
It may be said that the hyper-kinetic effec t of P D N acts on the higher part of the brain.
4) In the cases of the acute experiments, the slow waves in the neo-cortex E E G preceded to the seizure waves; the ropeated administration of P D N 10mg/kg caused the slow-wave in the neo-cortex E E G.
It may be said that the slow waves caused by the administration of P D N are the premonitor of cramp attack.5) Pseudocerebral phagocytes, slight infiltration and atrophy of the cerebral cells were observed in the cases of the repeated administration of P D N for 60 days, but t here is no evidence of edema and inflammation.

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Sedimentation Analyses of Ribosomes and Polyribosomes from the Posterior Silk Gland of the Silkworm, Bombyx Mori.

Noboru Yamaguchi, Eiichi Otsuki, Yutaka Tashiro

1971 Volume 23 Issue 1 Pages 127-131
Published: March 20, 1971
Released on J-STAGE: February 19, 2013

DOIhttps://doi.org/10.5361/jkmu1956.23.1_127

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Sedimentation propeties of ribosomes and polyr ibosomes obtained from the posterior silk gland of the domesticated silkworm, Bombyx Mori, (Heian X Gunpaku) have beer studied.
The posterior silk glands at the fifth larval instar were homogenized in the ice cold rat liver supernatant. The homogenate was centrifuged for 10 min at 800g (average) and then deoxycholate was added to the supernatant at a final concentration of 1 %.
This supernatant was analysed by sucrose density gradient centrifugation at 40,000 rpm for 60 min. using a swing bucket rotor Hitachi RPS-40, The same material was also analysed by a Spinco Model E analytical centrifuge.
The polyribosomal patterns up to pentam er were clearly detected in the beginning of fifth larval instar (24-48 hours old). At the later stage of the same instar, the amount of polyribosomes decreased gradually and at the end of the fifth larval instar, no polyribosomal pattern was detected.
This finding is not consis tend with the rate of biosynthesis of fibroin, and it is suggested that the increase in the amount of RNase activity of the posterior silk gland at the end of the fifth larval instar is probably responsible for the disappearance of the polyribosomes.
The standard sedimentation, coefficient at infinite dilution (S⁰₂₀, w) of the ribosome monomer from the posterior silk gland was found to be 79.6 S.

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Strophanthin-G Toxicity and Influences of Trimetazidine on Actions of Strophanthin-G on Guinea-pig Atria

Masako Kotani

1971 Volume 23 Issue 1 Pages 132-139
Published: March 20, 1971
Released on J-STAGE: February 19, 2013

DOIhttps://doi.org/10.5361/jkmu1956.23.1_132

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On the strophanthin-G toxicity and influences of trimetazidine on it, changes of inorganic ions content in isolated guinea-pig atria were examined.
1) The strophanthin-G toxicity was marke dly reduced in a low sodium medium and it was increased in a low potassium medium.
2) A potassium-free medium or a toxic dose of strophanthin-G caused a decrease in potassium content and an increase in sodium content in atria.
3) Trimetazidine prevented a decrease in potassium c ontent but could scarcely prevent an increase in sodium content in atria kept in a normal Locke's solution containing strophanthin-G in a toxic dose.
4) Trimetazidine may reduce strophanthin-G toxicity perhaps through inhibiting the efflux of atrial potassium.

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Effects of Quinidine and Thiamine tetrahydrofurfuryldisulfide on Electrocardiogram of Rabbit

Masako Kotani

1971 Volume 23 Issue 1 Pages 140-147
Published: March 20, 1971
Released on J-STAGE: February 19, 2013

DOIhttps://doi.org/10.5361/jkmu1956.23.1_140

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Quinidine and thiamine tetrahydrofurfuryldisulfide (TTFD) were investigated for their effects on ECG of rabbit in relation to interaction of these drugs. The QRS complex, P-Q interval and Q-T interval were prolonged after the intravenous injection of 17.5mg/kg of quinidine. TTFD injected intravenously in doses of 10 to 20 mg/kg prolonged R-R interval for 2 to 3 hours and depressed sinus node activity for 1 to 5 minutes. Premedication of TTFD in above doses reduced changes of QRS complex and P-Q interval caused by quinidine.

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Effects of Limbic System upon Photic Evoked Potentials in Rabbits.

Satoru Kubota

1971 Volume 23 Issue 1 Pages 148-164
Published: March 20, 1971
Released on J-STAGE: February 19, 2013

DOIhttps://doi.org/10.5361/jkmu1956.23.1_148

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It has been well known that the limbic system plays an important role in behavioral and emotional disorders. In relation to this concept, the effects of limbic stimulation upon photic evoked potentials were investigated.
Sixty-five rabbits were imm obilized by succinylcholine chloride, and respiration was artificially maintained. Electrodes were stereotaxically implanted in septum, hippocampus, amygdala, lateral geniculate body, superior colliculus and visual cortex. Twenty repeated evoked responses were averaged by average transient computer (ATAC).
Photic potentials evoked in bilateral visual cortex and later al geniculate body were accentuated or suppressed by preceding stimulation of septum. Preceding stimulation of hippocampus also facilitated or inhibited photic evoked potentials in bilateral visual cortex and lateral geniculate body. Amygdala had facilitatory effects on photic evoked potentials in bilateral visual cortex and lateral geniculate body. However, these interactions disappeared in association with intravenous barbiturate injection and in cerveau isole rabbits. These results clarify behavioral disorders concomitant with limbic irritation and also certify that the mesencephalic reticular formation takes an important part of these interactions.

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Report on A Case of Severe Agranurocytosis Induced by Methylthiouracil which Showed Leukemoid Reaction in Its Convalescent Stage

Terutoshi Kokawa, Yuruko Okamoto

1971 Volume 23 Issue 1 Pages 165-171
Published: March 20, 1971
Released on J-STAGE: February 19, 2013

DOIhttps://doi.org/10.5361/jkmu1956.23.1_165

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A case of severe agranulocytosis with leukemoid reaction induced by methylthiouracil was reported. The patient was a 26 years old nurse suffering from hyperthyroidism. She was admitted to our clinic with complaints of high fever and sore throat. From her history and laboratory findings, this: case was diagnosed as agranulocytosis induced by Methyl-thiouracil. The patient was successfully treated with ACTH, steroid hormone and fresh-blood-transfusion from a chronic myeloic leukemia patient. Leukemoid reaction was recognized in her convalescent stage.

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A Case of Submucosal Pancreatic Cyst in the Stomach Wall

Noboru Yamaguchi, Kazuo Tsunagawa, Takuji Iwama, Yoshichika Aosaki, Is ...

1971 Volume 23 Issue 1 Pages 172-176
Published: March 20, 1971
Released on J-STAGE: February 19, 2013

DOIhttps://doi.org/10.5361/jkmu1956.23.1_172

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A submucosal pancreatic cyst in the stomach wall, grown in the posterior wall of the central part of the antrum, was observed in a 22 years old male.
X-ray examination of the stomach demonstrated a semicircula r shadow defect in the antrum. Corresponding to that, a tumor was found endoscopically, which was considered to a submucosal one, although neither typical bridging folds nor central depression were shown. Beside, two ulcers were found at the gastric angle.
Resected stomach disclosed that the tumor, mea suring 40 X 37 X 15mm, was a submucosal cyst, which was later diagnosed histologically as an aberrant pancreatic tissue dilated like a cyst.

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