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Volume 17 (1965)
- Issue 4 Pages 301-
- Issue 3 Pages 213-
- Issue 2 Pages 123-
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Volume 17, Issue 4
Displaying 1-10 of 10 articles from this issue
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[in Japanese]1965 Volume 17 Issue 4 Pages 301-313
Published: December 20, 1965
Released on J-STAGE: February 19, 2013
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Pseudomonas aeruginosa in vaginal materialsKiyoko Takashina1965 Volume 17 Issue 4 Pages 314-325
Published: December 20, 1965
Released on J-STAGE: February 19, 2013
JOURNAL FREE ACCESSIn an attempt to isolate Ps. aeruginosa from vaginal materials, one hundred cases of the out-patients at the Mima Obstetrics and Gynecology Clinic have been studied bacteriologically by means of microscopic examination by the use of direct smear and by cultivation on the plates of nutrient agar and the glutamic acid medium.
Following results were observed.
1. The microscopic examination of vaginal materials revealed that in the cases of 2nd and 3rd grade of “Reinheitsgrad” the most of the bacteria other than Doderlein's bacilli were Gram positive short bacilli and Gram negative ones were very few.
2. Compared with the case of nutrient agar plate, only one half of the number of colonies appeared in the case of glutamic acid medium on which the streak culture of vaginal materials was made.
Gram positive bacilli were most frequently demonstrated by the microscopic examinations of these colonies on either medium. There was, however, a considerable difference between the microscopic findings of direct smear and postcultural colonies.
3. Two strains of Ps. aeruginosa were isolated from the vaginal material of two pregnant women, one in the second month of pregnancy and the other in the beginning of miscarriage of her 3rd month of pregnancy. Those strains were found to be typical as Ps. aeruginosa.
4. In the cases of two women whose vagina harboured Ps. aeruginosa, spontaneous recovery with a disappearance of bacilli was observed in the later examination and no evidence of pseudomonas infection was noted in other patients.
From these findings it can be said that as far as Ps. aeruginosa is concerned, the vagina of matured women is considerably clean, so that high percentage isolation of the organism from vagina and the occurrence of serious complications caused by pseudomonas will never come unless the organism is introduced from outside by such a factor as hospital infection.View full abstractDownload PDF (2140K) -
Effects of Amino Acids on the Production of Fluorescence II FractionKiyoko Takashina1965 Volume 17 Issue 4 Pages 326-337
Published: December 20, 1965
Released on J-STAGE: February 19, 2013
JOURNAL FREE ACCESSI. Observations were made on the growth and pigment production of eight strains of Ps. aeruginosa on the synthetic media, each of which contained one of the thirty three different kinds of amino acid and its delivatives. The results were as follows:
1) In the media which containedα-and β-alanine, glutamic acid, γ-amino-n-butyric acid, praline, all the test strains showed good growth and clear pigmentation, and especially the one which contained γ-amino-n-butyric acid revealed a pronounced effects.
2) In the media which contained asparagine, aspartic acid, glutamine, arginine, ornithine, glutaric acid, α-ketoglutaric acid and histidine, all the test strains showed a good growth, but less pigment and fluorescence production.
3) In the media which contained valine, leucine, isoleucine and tyrosine, some of the test strains showed a good growth with a moderate pigmentation.
4) In the media which contained glycine, serine, threonine, phenylalanine, α-and β-amino-n-butyric acids and citrulline, all the test strains grown poorly showing very small colonies after one week cultivation.
5) In the media which contained norleucine, cystine, cysteine and methionine, none of the test strains could grow.
II. Four strains (No.001,002,117 and 58) were cultivated on the synthetic liquid media, each of which contained one of the twelve kinds of amino acid, which allowed these four strains a good growth. After one week cultivation, the gel filtration for every culture filtrate were performed, collecting and analyzing the Fluorescence II fractions. The results were as follows.
1) Whenever all the strains tested showed good growth, Fluorescence II fraction was obtained regardless with the production of visible colour and Fluorescence I fraction.
2) Paper electrophoretic analysis of Fluorescence II fraction showed that the strain No.58 was able to produce the fluorescent substances, which were characteristic of Ps. aeruginosa, from almost all the amino acids tested.
3) The paper electrophoretic pattern of Fluorescence II of pyocyanin strain No.002was very similar to that of No.58 but melanin strains No.001 and 117 were quite different with that of No.58.View full abstractDownload PDF (2447K) -
Tu Yun Feng1965 Volume 17 Issue 4 Pages 338-342
Published: December 20, 1965
Released on J-STAGE: February 19, 2013
JOURNAL FREE ACCESSObservations were made on the phenomenon of heterohemagglutination of normal crawfish sera to the washed red cells of several vertebrates, such as human (Type A, B, O and AB), rabbits, guinea pigs, rats, a goat, sheep and fowl. The results are as follows:
1. From 92 to 100% of test sera agglutinated human, guinea pig, rat and fowl red cells distinctly, about 80% in the case of rabbit and sheep; but none of them showed positive reaction to goat red cells. The heterohemagglutinin-titer of crawfish serum showed highest in rat red cells.
2. When the crawfish sera was obserbed by washed human red cells (Type O), the positive reaction to rabbit, guinea pig and sheep cells disappeared in all the test sera; more than half cases of absorbed sera showed negative reaction to human A and O red cells. But the agglutinating factor to rat red cells remained positive.
3. It is reasonably considered that the heterohemagglutination of crawfish sera may be caused by hemocyanin fraction of the sera.
4. In the cases of other crustacean than crawfish, only Neptunus trituberculatus and Penaeus japonicus sera agglutinated rabbit and human (Type AB) red cells respectively.View full abstractDownload PDF (1057K) -
Detection of Ps. aeruginosa obtained from human pharynges and its resistance against several disinfectantsYoko Yukawa1965 Volume 17 Issue 4 Pages 343-348
Published: December 20, 1965
Released on J-STAGE: February 19, 2013
JOURNAL FREE ACCESSI. The detection of Ps. aeruginosa from human pharynges was tried, using glutamate plate medium; and noticed positive results 6 among 66 cases (9%) of pharyngitis patients,2 among 67 cases (3%) of nurses in our attached hospital and 3among 180 cases (1.7%) of our medical students. In healthy persons (nurses and students), only 5 among 247 cases (2%) were proved to be positive.
II. Isolated strains were identified taxonomically as Ps. aeruginosa according to their biochemical characteristics.
III. Four strains of Ps. aeruginosa preserved in our laboratory were tested about their resistance against phenol, saponated cresol and benzarconium chloride solution. The results were as follows:
1) The test organisms were killed almost perfectly by treatment with 1% or more solution of phenol or saponated cresol in 5 minutes.
2) In the case of benzarconium chloride solution, all of the 4 strains tested could servive in even the concentrated solution of 5% or more. One of the test strains was found resistent to the benzarconium solution from 0.001 % to 10%.
From the observation mentioned above, it is reasonable to conclude that Ps. aeruginosa is not a habitant organism in adult pharynges and that medical disinfectant must be carefully selected to protect the hospital infection caused by Ps. aeruginosa.View full abstractDownload PDF (1430K) -
Bacteriological Properties of Several Melanogenic StrainsToyoko Tanabe1965 Volume 17 Issue 4 Pages 349-356
Published: December 20, 1965
Released on J-STAGE: February 19, 2013
JOURNAL FREE ACCESSStudies were made on the biological and biochemical properties of 5 strains of melanin producing Ps. aeruginosa; 2 stock strains of our laboratory No.001 and No 117, Gessard's strains A 17 and A 18, and Liu's strain 1184. The results are as follows.
1. Strain A 18 showed not only diffused growth in semisolid agar deep, but also a polar flagella by Nishizawa & Sugawara's method of flagella staining. The other 4 strains revealed no flagella either in semisolid media or by flagella staining.
2. All the 5 strains tested showed good growth and brown pigmentation on nutrient agar media incubated either at 37°C or at 42°C. Observation of the characteristics of the colonies grown at 37°C revealed the fact that the colonies of A 17 and 1184were smaller than those of usual Pseudomonas strains, and the colonies of the other 3 strains showed medium or somewhat larger, flatt and irregular shaped.
3. Strains A 17, A 18 and 1184 changed Christensen's urea medium into red color within 4 hours after their heavy inoculation. Strain No.117 did not liquefy gelatin, and 1184 did not grow on Simmons' citrate medium.
4. Strain 1184 produced acid by oxidation of glucose only, and the other strains tested were the oxidizers not only of glucose but of several other kinds of carbohydrates.
5. The utilization of amino acids by melanogenic strains of Ps. aeruginosa were also studied. In the case of strain 1184, only 3 kinds of amino acid, arginine, ornithine and citrulline were utilized for growth of the bacteria, though the other 4 strains showed almost the same results as typical Ps. aeruginosa. All the 5strains tested produced neither melanin nor pyocyanin on the synthetic media containing single amino acid.
6. The Fluorescence II fraction of the tested strains cultivated in glutamic acid liquid medium did not show the typical paperelectrophoretic pattern of Ps. aeruginosa, but the characteristic pattern was revealed when cultivated in histidine medium.View full abstractDownload PDF (1612K) -
The Brown Pigment of Melanogenic Strain No. 117Toyoko Tanabe1965 Volume 17 Issue 4 Pages 357-363
Published: December 20, 1965
Released on J-STAGE: February 19, 2013
JOURNAL FREE ACCESSA melanogenic strain of Ps. aeruginosa, No.117, was inoculated to King A liquid medium, using Polypeptone (Takeda) as carbon and nitrogen sources, and incubated at 37°C for over 1 month. The Brownish black precipitate, obtained from the culture filtrate by adding the 20% CuSO4 solution, was dissolved in 1M NH4OH and fractionated by gel-filtration through the Sephadex G-25 column. Melanin I was obtanied by adding diluted acetic acid solution to Fraction I, and Melanin II was obtained by saturating Fr. II with methyl-ethyl keton.
Melanin I is amorphous black substance, much soluble in alkaline solution and a little in HCl 5 N or more, and insoluble in various organic solvents. Analytical found: Melanin I; C 44.6, H 5.37, N 11.35, calcd. for (C5H8NO2)n; Melanin II; C 51.36, H 6.09, N 11.15, calcd. for (C5H7NO2)n. Spectrophotometric studies of Melanin I revealed no specific absorption.
Hydrolysis of Melanin I revealed the presence of 13 amino acids which, by twodimensional chromatography, could not be distinguished from glycine, alanine, vialne, leucine, serine, phenylalanine, tyrosine, tryptophan, proline, arginine, histidine, glutamic acid and aspartic acid.
The alkaline solution of the hydrolysis-residue was brownish and showed no electrophoretic mobility at pH 8.6. Both the Melanin I and its hydrolysis-residue, dissolved in alkaline solution, were faded by hydrogen peroxide and changed into three or more unknown fluorescent substances.View full abstractDownload PDF (1328K) -
Takahiko Asayama1965 Volume 17 Issue 4 Pages 364-378
Published: December 20, 1965
Released on J-STAGE: February 19, 2013
JOURNAL FREE ACCESSIt was demonstrated that there exists a cholinergic sympathetic vasodilator inneron the blood vessels of the retina and external eye muscle of bullfrogs.
These cholinergic sympathetic vasodilator fibers originate from the spinal cord passing through the posterior spinal roots II, III and the sympathetic chain.
It is revealed that the cholinergic vasodilator fibers in the sympathetic chain become predominant over the adrenergic vasoconstrictor fibers in the sympathetic chain following a pretreatment with Reserpine.
It is confirmed that the adrenergic vasoconstrictor fibers to the blood vessels of the retina and external eye muscle of bullfrogs originate from the spinal cord passing through the anterior spinal roots II, III and enter the sympathetic chain together with cholinergic sympathetic fibers from the posterior spinal roots II, III.View full abstractDownload PDF (5202K) -
Hidemi Yamada, Reiko Yamada, Toru Mine, Eiko Kanayama, Jiro Sugimoto1965 Volume 17 Issue 4 Pages 379-385
Published: December 20, 1965
Released on J-STAGE: February 19, 2013
JOURNAL FREE ACCESSIsoxsuprine (1- (P-hydropheny1)-2-(1'-methyl-2'-phenoxy-ethylamino) propano1monohydrochloride (Duvadilan) was pharma-cologically Investigated and was appnea for 23 cases of ophthalmological patients suffered from occulsion of retinal arteries (3 cases), central chorioretinitis (6 cases), sclerosis of retinal central arteries (5cases), diabetic retinopathy (4 cases) and atrophy of optic nerve (5 cases).
Isoxsuprine is adrenergic, but has vasodilatative effect due to its adrenergic betaeffect, which was shown in animal experiments in this paper.
The effect of this drug on patients was symptomatical and it was used to improve the blood supply of retinal tissue which had been fallen in ischemia induced by above mentioned diseases. Though retinal arteries were dilated after intramuscular administration in dose of 5 mg, the cure effect was indistinct, but the drug was more useful and safe than any other vasodilator: amil-nitrit, imidaline or carnige n which has been recommended on these diseases.View full abstractDownload PDF (2512K) -
Toru Mine, Satoshi Hata, Tadaya Tsuboi, Ichiro Fujii, Hidemi Yamada1965 Volume 17 Issue 4 Pages 386-392
Published: December 20, 1965
Released on J-STAGE: February 19, 2013
JOURNAL FREE ACCESSThe effect of Mydrin M, a cycloplegic with O.4%tropic acid N-ethyl-N-(γ-picolyl) amide as the chief component, on the visual acuity of 72 myopic children in Kyoto Kagamiyama Primary School was reported.
After one month of the treatment with Mydrin M instilled once a day before going to bed, it was found that the visual acuity of 58 eyes was remarkably,43slightly improved, but 13 eyes failed.
After one year of the treatment, the visual acuities were improved in 79% of the myopic children, and yet no side-effects were obserevd.
From the result of this experiment, it is concluded that Mydrin M is an effective agent for the treatment of pseudomyopia and the prevention of progress of refractive myopia in the children.View full abstractDownload PDF (1241K)
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