For many years, it was interested to study the effect of protein or amino acids contents of daily diet on the V. B₆ requirement, since vitamin B₆ has important roles on the metabolism of nutrition components, specially amino acid.
In this study rats fed on the diet that was supplemented 2% excess dl-methionine,1.6% excess l-cystine or 2.2% excess l-tyrosine for four weeks was determined those liver pyridoxal phosphate content and enzyme activities concerned the pyridoxal phosphate formation. After the initial temporary increase, the liver pyridoxal phosphate content decreased since third day, gradually recovered and was equilibrated on a little increased value for normal one at the end of third week. It seemed that this variation of liver pyridoxal phosphate content was given by the change of the pyridoxal kinase activity (the pyridoxal kinase is one enzyme concerned pyridoxal phosphate formation). The activity of pyridoxine phosphate oxidase was simultaneous assayed, but no change was found.
Cerecedo et al. reported that vitamin B₆ deficient rats fed on this amino acids supplemented diet indicated the early appearance of deficient symptom, but not on other amino acids supplemented diet. But in this study, dl-methionine, l-cystine and l-tyrosine gave a similar effect on liver pyridoxal phosphate content.
For the initial temporary increase in the liver pyridoxal phosphate content and pyridoxal kinase activity, it was suggested to adapt the change of diet components. Then the hormonal effect of the initial temporary increase in liver pyridoxal phosphate content and pyridoxal kinase activity was investigated. The cortisone injection independently effected to increase them. Both adrenalectomized and pituitarectomized rats were also determined. Based on those investigations, the initial temporary increases in liver pyridoxal phosphate content and pyridoxal kinase activity were suggested to be effected with adrenals.

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Kotake et al. observed that when tryptophan was given to rats fed on a high fat diet or administered with a large dose of various fatty acids, there was a remarkable increase in urinary xanthurenic acid excretion. Charconnet-Harding et al. also reported the increased excretion of some tryptophan metabolites including xanthurenic acid in the urine of rats fed on a vitamin B₂ deficient diet. It was agreed in several workers that the abnormal increase of xanthurenic acid excretion in urine was due to vitamin B₆ deficiency. Thus, a close relationship between the roles of vitamins B₂ and B₆ in tryptophan metabolism could be easily inferred. However, no definite explanation could be given to this metabolism.
According to recent reports of Ichihara et al., in mammalian tissues pyridoxal phosphate is formed from pyridoxine via pyridoxine phosphate and pyridoxine phosphate oxidase is a flavoprotein.
In the present works it was examined on the effect of a high fat diet and fatty acid administration on the activity of pyridoxine phosphate oxidase and other enzymes concerned with pyridoxal phosphate formation in rat liver in an effort to clarify the mechanism of the effect of fatty acids. The liver pyridoxal phosphate content and pyridoxine phosphate oxidase activity of rats fed on a high fat diet were determined weekly. They were found to decrease gradually. The change in pyridoxine phosphate oxidase activity seemed to precede that of the pyridoxal phosphate content. This suggests that pyridoxal phosphate formation decreased as a consequence of decreased pyridoxine phosphate oxidase activity. Those depressions both of liver pyridoxal phosphate content and the pyridoxine phosphate oxidase activity were prevented with FMN administration.
Next, the liver pyridoxal phosphate content and pyridoxine phosphate oxidase activity of rats administered oral with various fatty acids were measured and also decreased.
After oral administration of sodium butyrate, the variation of liver pyridoxal phosphate content and pyridoxine phosphate oxidase activity with time investigated, both indicated parallel variation and later returned to normal levels. As in the case of fat feeding, the effect of fatty acids administration was prevented with simultaneous injection of riboflavin.
Also tne activity of xanthine oxidase, a flavine enzyme, was found to be depressed under similar conditions, futhermore addition of FAD in vitro restored the depressed xanthine oxidase activity. Those findings emphasize the fact that fatty acid administration causes a relative vitamin B₂ deficiency.

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The fact that Ps. aeruginosa produces blue color, pyocyanin, has been well known and is one of the important indecies for the clinical detection and isolation of the bacilli. But many strains of Ps. aeruginosa produce yellow, red or brown color together with or instead of pyocyanin, and some other strains show no pigment visible in ordinary light.
Though these findings are quite important in the clinical examination they were left scarcely noticed up to the present.
In such a situation, Prof. Osawa and his colleagues devised a simple synthetic medium containing glutamic acid, suitable to observe the pigment production of Pseudomonas group. In this paper, results will be presented on the isolation of Ps. aeruginosa from feces by the use of the glutamic acid medium and the comparison of pseudomonal coloration between our medium and King A & B media on the 50 strains preserved in our laboratory.
On the glutamic acid medium, pigment and fluorescens always appear more rapidly and the colors are more brilliant than that on King's media. When the patients' specimen (feces) is smeared on the glutamic acid medium the coloration and fluorescence in an early stage of cultivation made the isolation of Pseudomonad easy and precise.
Moreover, as far as the preserved strains are concerned, various kinds of cocci and bacilli other than Pseudomonad hardly grow on this medium.
From these findings it is considered that the glutamic acid medium is very suitable not only for the observation of pigment production but also for the isolation of Ps. aeruginosa.

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Eighteen asthmatic patients were tested with antigens of house dust, mold, tree, grass and ragweed by the Anderson Sampler at National Jewish Hospital, Denver, Colorado.
(1) Anderson Sampler: This apparatus is devised to measure the exact quantity of an antigen inhaled and retained by the subject during an inhalational challenge test. It consists of nebulizer on the right which is connected by a series of tubes to an Anderson Sampler on the left. A known quantity of an antigen is mixed in the nebulizer with a known quantity of phenol red, used as a tracer dye, and 2 1/2% glycerol used to reduce vapor pressure and thus stabilize particle size. The material is then nebulized at a constant rate for five minutes.
At the end of this period, the optical density of the material in the sampler is carefully washed with a known amount of 0.05 normal sodium hydroxide solution and measured using a spectrophotometer.
In the third run, exactly the same procedure is repeated with only one difference; this time the patient is connected to the apparatus in such a way that while the nebulizer is running, he inhales from it for exactly five minutes. At the end of that period, material collected in the Anderson Sampler is measured again. This time, as expected, the optical density of the material collected in the sampler would be less than that in the original run. The amount which is lost has been obviously retained by the patient. No red dye is found in the mouth or the throat of the subject.
(2) The results are as follows:
1. Nine patients had a decreased FEV₁ between 26-60%, from 7-90 PNU of house dust. One of these had a shock reaction from 31 PNU of the house dust.
2. Seven patients showed a positive reaction from 16-263 PNU of mold and a decreased FEV₁ of 24-68%. One of these had a shock reaction from 59 PNU.
3. Five patients had a positive reaction from 23-251 PNU of tree antigen, however, no one fell in to shock.
4. From the grasses antigen, ten patients showed a positive reaction with 15-310 PNU; and decreased FEV₁. of 20-78%.
5. Ten patients had a reaction from 12-252 PNU of ragweed antigen and decreased FEV₁of 18-81%. Two of these had a shock reaction from 101 PNU and 142 PNU.
When we compared each allergen,50-55% of 18 patients showed a positive reaction to house dust, ragweed, grass, while 28-39 % of patients showed a positive reaction to mold and tree.
Looking at these reaction, we can see that the positve reaction to the tree allergen is weak, while the positive reaction to the ragweed allergen is strong.
With house dust, a positive reaction occured in all patients to a relatively small amount of allergen.
From our testing, we have determined that the house dust and ragweed allergens are the most significant to allergic asthma, and that there is no definite relationship between the amount of allergen and the degree of asthmatic reaction. Therefore the difference in degree of bronchial asthma depends on the bronchial sensitivity to particular antigens.

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It was reported some time ago that streptomycine or isoniazid depressed tuberculine sensitivity. Since then many accounts have been reported about it.
Hewell reported that during isoniazid therapy, there was a decrease of tuberculine sensitivity. During 1960, in New Mexico, there were reports indicating that approximately 15% of the students who had positive tuberculine reaction converted into negative after isoniazid therapy.
We had an experience where two children tuberculosis patients who had tuberculine reaction had converted after eight months of isoniazid treatment. These two patients were admitted to our hospital because of positive tuberculine reaction without change in chest X-ray. We have not seen any change in tuberculine reaction in the other patients with positive tuberculine reaction with change in the chest X-ray.
That tuberculine sensitivity is diminished by isoniazid treatment especialy in simple pulmonary tuberculosis means that the hypersensitivity of tuberculine reaction is diminished by isoniazid.

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Since it's discovery, prednisone, combined with chemotherapy, is often used for the treatment of tuberculosis. It has become one of the important agents in the treatment of acute tuberculosis or tuberculous meningitis where the patient is in the acute stage.
It has also been used for the treatment of children's pulmonary tuberculosis, and the reports have shown fair results.
We were interested in knowing if there were any effects on tuberculine sensitivity during the use of this agent combined with chemotherapy on tuberculous patients.
Eight patients were selected for this purpose because of positive tuberculine reaction even after chemotherapy for eight months.
5mg Qid, for four days,10mg Tid, for three days of prednisone were ordered. On the fifth day after prednisone treatment was started, Mantoux 1: 10,000 OT was tested on each patient.
We know that short term prednisone combined with chemotherapy will depress tuberculine sensitivity in some patients, however not in all.

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Methyl methionine sulfonium chloride (MMSC), homocysteine thiolactone (HCT) and L-methionine (l-Meth) were administered to rabbits with experimental fatty liver due to cholesterol, in an attempt to determine serum and liver lipid fractions (cholesterol, ester cholesterol, total phospholipid, total fatty acid, neutral fat, total fat, lecithin, cephalin and sphingomyelin).
The liver and serum lipid fractions of an MMSC administered group show a marked decrease of total fatty acid, neutral fat and total fat than HCT and l-Meth administered groups. The liver and serum lipid fractions of l-Meth administered group show a marked decrease of cholesterol and an increase of lecithin over the other groups.
An MMSC administered group seems to show a more favorable lipotropic action for cholesterol fatty liver than HCT and l-Meth administered groups. It is to be noted that the HCT administered group scarcely showed lipotropic action. A review was made of fatty liver and lipotropic substances.

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The influences of l-methionine and methylmethionine sulfonium chloride on the rhythmic contractions of the isolated rabbit and guinea-pig atria were examined.
l-Methionine increased the effects of endogenous and exogenous adrenaline and acetylcholine on the atria, while methylmethionine sulfonium chloride decreased them. It was discussed that l-methionine may be one of the physiologic factors regulating the autonomic functions of atria and methylmethionine sulfonium chloride may interfere in the biochemical and pharmacological actions of l-methionine on the atrial mechanism.

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