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Volume 54 (2002)
- Issue 2-4 Pages 119-
Volume 54, Issue 2-4
Displaying 1-8 of 8 articles from this issue
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Satoshi Mizutani, Aiko Umemoto, Farjana Jahan Fattah, Kazi Riaz Fattah ...2002 Volume 54 Issue 2-4 Pages 119-123
Published: December 30, 2002
Released on J-STAGE: February 19, 2013
JOURNAL FREE ACCESSyciM, one of the uncharacterized ORFs in Escherichia coli, located next to the DNA replication terminus terA, was found to suppress a suppressor sensitive crp- mutant in 2 copies, one placed in trans in a single copy plasmid and the other on the chromosome in cis, but not in single copy on the chromosome. Moreover, it seems to be lethal to the host in multiple copies, because it could not be subcloned in a multicopy vector. The suppression sensitive crp mutation was leaky and had been credted by the insertion of an 152 element between the crp promoter and its coding region.View full abstractDownload PDF (1747K) -
Hisae Gemba, Shin-ichi Kyuhou, Ryuichi Matsuzaki, Yae Amino, Kazuko Ma ...2002 Volume 54 Issue 2-4 Pages 124-129
Published: December 30, 2002
Released on J-STAGE: February 19, 2013
JOURNAL FREE ACCESSRecording field potentials during learning processes of audio-initiated vocalization by electrodes implanted chronically on the surface and at 2.0-3.0 mm depth of the cerebral cortex and analyzing them seem useful for knowing central nervous mechanisms of the vocalization in the monkey. We have been waiting for an opportunity to record the potentials during the motor learning in a short period, because it usually takes a considerable time (3-6 months) for a naive monkey to be trained well for the vocalization. One of three monkeys, which had been fully trained for the vocalization, was found to be unable to perform the vocalization after electrode-implanting operation. We could record the potentials in the monkey during the process of learning the vocalization again. The learning process of the vocalization partly differed from audio-initiated hand movements previously reported, and there was no skill learning in the vocalization. The right cerebellar hemispherectomy eliminated the surface-negative, depth-positive (s-N, d-P) premovement potential recorded before the operation in the left face motor cortices in the three monkeys, and changed vocal tone. Scarce change was seen in reaction times in audio-initiated vocalizations before and after the operation, again differing from audio-initiated hand movements. This suggests that central nervous mechanisms in audio-initiated vocalizations differ from those in audio-initiated hand movements. These nervous mechanisms were discussed in connection with the limbic system.View full abstractDownload PDF (1122K) -
Hisashi Ito, Satoshi Nakano, Hidefumi Ito, Hirofumi Kusaka2002 Volume 54 Issue 2-4 Pages 130-134
Published: December 30, 2002
Released on J-STAGE: February 19, 2013
JOURNAL FREE ACCESSMuscle Afferent Block (MAB) has been shown to improve clinical symptoms of writer's cramp, cervical dystonia, spastic paraparesis, and oromandibular dystonia. However, this therapy is not yet used to a sufficient extent. In this article, we review our experience with this new treatment The subjects were 11 cases encountered between November,2001 and April,2002, including 4 cases of focal dystonia of an upper limb,3 cases of spastic paralysis of an upper limb,3cases of spastic paraparesis, and 1 case of spasmodic torticollis. All patients exhibited some clinical improvement. Two cases of facial flushing and 3 cases of muscle weakness occurred as side effects. All of them were mild and transient. We conclude that MAB is a useful treatment for dystonia and spasticity with low incidence of side effects.View full abstractDownload PDF (815K) -
[in Japanese]2002 Volume 54 Issue 2-4 Pages 135
Published: December 30, 2002
Released on J-STAGE: February 19, 2013
JOURNAL FREE ACCESS -
Taketoshi Kushida, Muneo Inaba, Hiroko Hisha, Naoya Ichioka, Takashi E ...2002 Volume 54 Issue 2-4 Pages 136-149
Published: December 30, 2002
Released on J-STAGE: February 19, 2013
JOURNAL FREE ACCESSWe have succeeded in treating intractable autoimmune diseases in chimeric resistant MRL/lpr mice by a new bone marrow transplantation (BMT) method consisting of fractionated irradiation (5.5 Gy x2) followed by intra-bone marrow (IBM) injection of whole bone marrow cells (BMCs) from allogeneic normal C 57 BL/6 (B 6) mice [5.5 Gy X2+ IBM]. In MRL/Ipr mice treated with this method, the number of not only donor-derived cells but also donor-derived hemopoietic progenitor cells increased. Furthermore, donor-derived stromal cells were clearly detected in the cultured bone pieces from MRL/lpr mice treated with [5.5 GyX2+IBM]. All the recipients thus treated survived more than one year (60 weeks after birth) and remained free from autoimmune diseases. Autoantibodies decreased to almost normal levels, and abnormal T cells (Thy 1.2+/B 220k+CD 4-/CD 8-) disappeared. These findings clearly indicate that this new strategy (IBM-BMT) creates the appropriate hemopoietic environment for the early recovery of hemopoiesis and donor cell engraftment, resulting in the complete amelioration of intractable autoimmune diseases in chimeric resistant MRL/lpr mice without recourse to immunosuppressants. This strategy would therefore be applicable to human therapy.View full abstractDownload PDF (3857K) -
[in Japanese]2002 Volume 54 Issue 2-4 Pages 150-156
Published: December 30, 2002
Released on J-STAGE: February 19, 2013
JOURNAL FREE ACCESSDownload PDF (922K) -
[in Japanese]2002 Volume 54 Issue 2-4 Pages 157-163
Published: December 30, 2002
Released on J-STAGE: February 19, 2013
JOURNAL FREE ACCESS -
Yoshinobu Sugino2002 Volume 54 Issue 2-4 Pages 164-176
Published: December 30, 2002
Released on J-STAGE: February 19, 2013
JOURNAL FREE ACCESS1. The genes for Verotoxins are all located in the same position in lambdoid phages, and the so-called chromosomal Verotoxin or Shigatoxin genes so far sequenced are actually located on defective or cryptic pha g e genomes integrated in the bacterial chromosome; therefore they should be considered to be proper phage genes rath e r than genes of bacterial origin picked up secondarily in lambdoid phages.
2. In a lambdoid phage genome, a protein and its target DNA site interact through specific elements contained in the respective molecules. The gene for the protein and its target DNA site remain together through recombination because when separated they can no longer interact due to difference in specificity and become non-fun c tional.
3. Because lambdoid phage genomes are very small as a genome, and there are so vast accumulation of data on lambda, they are excellent material for refining and testing comparative methods: in other words, methods an d conclusions tested or obtained with them may be applicable to the still vaster genome information on vario u s organisms expected to be collected in the near future.View full abstractDownload PDF (1995K)
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