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Volume 19 (1973)
- Issue 6 Pages 469-
- Issue 5 Pages 369-
- Issue 4 Pages 289-
- Issue 3 Pages 187-
- Issue 2 Pages 95-
- Issue 1 Pages 1-
Predecessor
Volume 19, Issue 3
Displaying 1-10 of 10 articles from this issue
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Kunio MATSUI, Kazunori YANAGIYA1973 Volume 19 Issue 3 Pages 187-191
Published: 1973
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSFlavin content in cat, dog choroids and cat tapetum were estimated. The content in cat choroid was nearly as high as that in the cortex of cat kidney and about 11 times of that in dog choroid. The flavin con-tent in cat choroid was related to the presence of melanin, and that in white choroid of cat was very low. The content in cat tapetum was not so high as reported by other investigators. The difference in flavin content between right and left choroids of cat was insignificant. It was also shown that the major flavin in cat and dog choroids is unphosphory-lated RF2.View full abstractDownload PDF (428K) -
Kunio MATSUI, Kazunori YANAGIYA1973 Volume 19 Issue 3 Pages 193-204
Published: 1973
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSThe association of RF2 with melanin from cat and dog choroids was shown by dialysis and spectrophotometric methods. The association obeyed Freundlich's isotherm, and RF was liberated from RF-melanin complex by gel chromatography and by addition of CPZ. The liberation of flavin from cat choroid was not affected by Pronase treatment, though that from kidney was accelerated. It was also shown that flavins in cat choroid are not degraded by illumination. From above findings it was deduced that most of flavin in cat and dog choroids is associated with melanin.View full abstractDownload PDF (1024K) -
Masako YAMADA, Tohru TSUKAHARA1973 Volume 19 Issue 3 Pages 205-214
Published: 1973
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSA cup-plate technique was proposed for the microbioassay of inositol in natural products with Kloeckera apiculata as test organism.
The optimum conditions for the assay were preferred as follows. Assay medium: Atkin's basal medium with minor modifications and 1% agar, l.5-mm plate thickness, pH 6.0. Standard solution of inositol: pH 6.0-6.5. Inoculum size of test organism: 0.1 optical density in final concentration. Incubation: 27°C, 20-24 hr.
In this method, a reproducible, clear and sharply defined growth zone was steadily obtained and a linear response to pure inositol solution containing 10-400μg per ml was demonstrated with a probable error of 10% on statistical analysis. The inositol contents in some natural products obtained by the plate method were nearly equal to those determined with the turbidimetric one and satisfactory results were also recorded in the recovery experiments. This method is simple, accurate and available for the routine assay of inositol in biological materials of high potency.View full abstractDownload PDF (1885K) -
Hisateru MITSUDA, Kenji NAKAJIMA1973 Volume 19 Issue 3 Pages 215-227
Published: 1973
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSThe effects of a guanine analogue, 8-azaguanine, on the riboflavin biosynthesis and the nucleotide pools were examined using non-growing cell systems of flavinogenic and non-flavinogenic strains of E. ashbyii.
1) 8-Azaguanine, as such, strongly inhibited riboflavin formation at low concentrations. The halfway inhibition point was detected at 5×10-5 M.
2) The inhibition of riboflavin biosynthesis by 8-azaguanine was completely overcome by the supplementation of purines. Xanthine gave the most efficient recovery from this inhibition.
3) Fluctuation of the nucleotide pools in non-growing cells of flavinogenic and non-flavinogenic strains were examined using Dowex 1×2 (formate) in the presence of 8-azaguanine 18 hr after start of incubation. As a result, five new peaks appeared on the chromatogram in the flavinogenic strain whilst only one new peak was observed in non-flavinogenic strain.
Furthermore, with the addition of xanthine with 8-azaguanine, peaks I, II and IV disappeared from the column, peak III was unchanged and peak V decreased to a fair extent.
4) Five peaks in flavinogenic strain were identified to be (I) uridine, (II) 8-azaguanine, (III) guanosine, (IV) 8-azaguanosine and (V) 8-azaguanosine triphosphate in the order of elution from the column and one peak in non-flavinogenic strain was found to be 8-azaguanine.View full abstractDownload PDF (1133K) -
Fuminori MASUGI, Yutaka SUMI, Shoichi SHIMIZU, Saburo FUKUI1973 Volume 19 Issue 3 Pages 229-236
Published: 1973
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSPyridoxal 5'-phosphate(PLP)is known to combine with bovine serum albumin to form a (1:1) complex which scarcely dissociates, even when subjected to intensive dialysis. When this complex was incubated with apo-aspartate aminotransferase (apoCOT)for an appropriate time and the preincubated mixture then submitted to the usual GOT assay, the appearance of GOT activity was obviously confirmed, indicating that PLP was transferred from the albumin-PLP complex to apoGOT. The affinity (Km)of the albumin-PLP complex for apoGOT was 1.56μM. Simultaneous addition of α-ketoglutarate to the preincubation mixture decreased markedly the transfer of PLP. Albumin-PLP (1:1) com-plex showed its absorption peak at 332nm. Although no appreciable change was observed in the absorption spectrum of the complex when incubated with apoGOT, the fluorescence spectrum of the mixture excited at 330nm was remarkably different from that of the complex alone. On addition of DL-erythro-β-hydroxyaspartate to the incubated mixture of albumin-PLP complex with apoGOT, a new absorption peak at 492 nm, assignable to a dead-end deprotonated Schiff base between holoGOT and the substrate analog, appeared immediately. These facts strongly suggest that PLP was transferred by interaction of the albumin-PLP complex With apoGOT to yield holoGOT-substrate Schiff base on addition of substrate.View full abstractDownload PDF (670K) -
D. S. MURDOCK, C. J. GUBLER1973 Volume 19 Issue 3 Pages 237-249
Published: 1973
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSTotal thiamine, free thiamine, thiamine diphosphate (TDP) plus thia-mine triphosphate (TTP) and total hydroxyethylthiamine (HET) levels were measured in brains of control, deficient, and oxythiamine (OTH)- and pyrithiamine (PTH)-treated rats. The brain levels of TDP+TTP decreased to 39% and 12% of the control thiamine levels in defici-ent and PTH-treated rats respectively. The brain HET and TDP+TTP levels of the OTH-treated rats were not significantly different from the controls. The HET levels were decreased significantly (α<0.005) by the fourth day of treatment and decreased steadily to 1/7th of the control values in the terminal stages only in the PTH-treated rats. A significant drop in the HET levels from the control levels was interpreted to mean that the pyruvate utilization was significantly impaired in the brain.View full abstractDownload PDF (1107K) -
I. PROPERTIES OF THE REACTION PRODUCTSTeruko FUJIWARA-ARASAKI, C. B. COULSON1973 Volume 19 Issue 3 Pages 251-262
Published: 1973
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSFairly typical Maillard-type reaction products were obtained from the lysine-lactose browning system. These substances gave positive ninhy-drin (17), orcinol (16) and LOWRY (19) reactions and fraction D1, P1 and P3 showed fairly higher sugar content. Fractions D, and P1 contain 22.4 and 21.8% of sugar, respectively. Fractions D1 and P1 yielded 6.95 and 9.44% of free lysine, respectively, i. e. this corresponded to 25 and 24% of their original total nitrogen levels. A preliminary in vitro test of the fractions (P1 and P3) using M. tuberculosis H37Pv suggests some growth stimulation properties.View full abstractDownload PDF (908K) -
Kazuki KANAZAWA, Tomohiko MORI, Setsuro MATSUSHITA1973 Volume 19 Issue 3 Pages 263-275
Published: 1973
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSOxygen was absorbed during the process of decomposition of LAHPO to their SP. The oxygen absorption by LAHPO as well as oxidizing LA was retarded by the addition of antioxidants. The kinetics studies of oxygen absorption reaction were carried out using a polarographic ele-ctrode when LAHPO was in excess or by a respirometer when oxygen was in excess. In both cases, the reaction followed the first-order kinetics. Total amount of the absorbed oxygen by LAHPO was 1.26 moles per mole of LAHPO, that is, the stoichiometric ratio of the reaction of LAHPO with oxygen was 1 to 1. When LAHPO was incubated under oxygen-free atmosphere in the presence of a catalyzer, the decomposition of LAHPO was remarkably reduced. Thus, the oxygen absorption at the degradation steps of LAHPO was due to LAHPO itself and not to SP, and LAHPO was decomposed by the oxygen action. A possible scheme containing linoleic acid dihydroperoxide which was a reaction inter-mediate at the degradation process of LAHPO was discussed.View full abstractDownload PDF (1098K) -
Keiichiro MURAMATSU, Hisanao TAKEUCHI, Kyosuke SAKURAI1973 Volume 19 Issue 3 Pages 277-285
Published: 1973
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSThis study was conducted to determine the effect of feeding graded levels of dietary lysine on weight gain and free amino acid concentra-tions in blood plasma and liver of weanling rats. Animals were fed on diets containing 11.6% wheat gluten (equivalent in nitrogen to a 10% casein diet) supplemented with graded levels of L-lysine HCl (0 to 10%) for 14 days. An outline of the results obtained follows:
1) Maximum weight gain was observed with the groups fed the lysine supplement in the 0.64 to 1.8% range. Growth declined with further in-crease of dietary lysine.
2) Blood and liver were sampled after decapitation 6 hr after the final feeding on the 14th day, and amino acid concentrations were determined. Plasma lysine concentrations rose rapidly as dietary lysine increased and reached the maximum level with the 1.8% lysine supplementation. With the large supplement diet, the lysine con-centrations maintained a plateau. Plasma threonine levels were high when dietary lysine was low.
3) Similar responses of lysine and threonine concentrations in liver to the increase of dietary lysine was also observed, but the effect was markedly less than those of plasma. Most other amino acids in plasma and liver were nearly unchanged even when the dietary lysine was varied over a wide range.View full abstractDownload PDF (772K) -
Shigeru ONO, Hiroko HIRANO, Kijuro OBARA1973 Volume 19 Issue 3 Pages 287-288
Published: 1973
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSDownload PDF (167K)
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