Colon diseases can be affected by several factors such as gender difference and dietary supplemental vitamin B
6 (B
6). The nutritional status of B
6 is affected by gender difference, leading us to hypothesize that gender difference affects colon luminal environment, which is dependent on B
6 status. To investigate this hypothesis, we fed male and female rats a diet containing 1 mg, 7 mg, or 35 mg pyridoxine HCl/kg diet for 6 wk. We found significantly higher fecal mucin levels in female rats compared to those in male rats. Supplemental B
6 significantly increased fecal mucins and was particularly profound in the female rats. The abundances of cecal and fecal
Akkermansia muciniphila (mucin degrader) were unaffected. The fecal mucin levels were significantly correlated with colonic free threonine and serine and with gene expression of colon MUC16, implying that the combined effect of gender and dietary B
6 on fecal mucins was mediated by the alteration in the levels of such amino acids and MUC16 expression. This study further showed the significant effects of gender difference on colonic free amino acids such as threonine, ornithine, asparagine/aspartate ratio, and glutamine/glutamate ratio, cecal and fecal
Lactobacillus spp. levels, and colonic gene expressions of MUC16 and TLR8, the factors relating to colon health and diseases. Therefore, our findings suggest that gender difference and dietary B
6 may have an impact on colon diseases by modulating these parameters.
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