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Volume 27 (1981)
- Issue 6 Pages 513-
- Issue 5 Pages 395-
- Issue 4 Pages 263-
- Issue 3 Pages 165-
- Issue 2 Pages 77-
- Issue 1 Pages 1-
Predecessor
Volume 27, Issue 6
Displaying 1-10 of 10 articles from this issue
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Kraisid TONTISIRIN, Aree VALYASEVI1981 Volume 27 Issue 6 Pages 513-520
Published: 1981
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSDownload PDF (655K) -
Shuichi KIMURA, Yuji TAKAHASHI1981 Volume 27 Issue 6 Pages 521-527
Published: 1981
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSThe preventive effects of L-ascorbic acid (V.C) and pantothenic acid (PaA) against photosensitized hemolysis in the presence of pheophorbide a or hematoporphyrin were investigated in vitro. The development of hematoporphyrin-induced photosensitivity and the preventive effects of V. C and PaA were also investigated in male mice. V. C and PaA exerted preventive effects against photosensitized hemolysis in the presence of pheophorbide a or hematoporphyrin. In such cases, V. C and PaA had not only an additive effect but also a synergistic one. The time required for the photosensitized acute shock-like death (i.e., survival time), was significantly extended by simultaneous administration of V. C and PaA. This supports the findings of in vitro experiments.View full abstractDownload PDF (622K) -
Tae SAKURAI, Miyako ABE, Seiji HORI, Makoto MATSUDA1981 Volume 27 Issue 6 Pages 529-538
Published: 1981
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSThe in vivo effects of semicarbazide (SC) and aminooxyacetic acid (AOAA) on the B6 vitamer concentrations in brain subcellular fractions, especially in extrasynaptosomal, synaptosomal plasma, extrasynaptosomal and synaptosomal mitochondria, were investigated. Treatment of mice with SC decreased levels of pyridoxal phosphate (PLP) in extrasynaptosomal and synaptosomal plasma, while it had no effect on those in extrasynaptosomal and synaptosomal mitochondria. On the contrary, treatment with AOAA more significantly decreased pyridoxamine phosphate (PMP) levels in extrasynaptosomal and synaptosomal mitochondria than those in extrasynaptosomal and synaptosomal plasma. According to these results, the difference between in vivo effects of SC and AOAA on vitamin B6-enzymes was discussed.View full abstractDownload PDF (926K) -
Toshio OKANO, Atsuko TAKEUCHI, Tadashi KOBAYASHI1981 Volume 27 Issue 6 Pages 539-550
Published: 1981
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSA simplified method for the determination of vitamin D2 in fortified dried milk was established by using two steps of highperformance liquid chromatography (HPLC). About 1g of fortified dried milk was accurately weighed and directly saponified. The extracted unsaponifiable matter was first subjected to preparative HPLC using a Nucleosil 5C18 column (reversed-phase type) with acetonitrile-methanol (1:1) as a mobile phase and a fraction containing vitamin D2 was separated. The separated fraction was subsequently subjected to analytical HPLC using a Zorbax SIL column (straight-phase type) with 0.4% isopropanol in n-hexane as a mobile phase. Since the peak corresponding to vitamin D2 was clearly observed with separation from other concomitants on the chromatogram of the analytical HPLC, the vitamin was assayed by estimating the peak height. The overall recovery of vitamin D2 by the proposed method was 94.3±2.3% (mean±SD). Naturally occurring vitamin D3 derived from cow's milk was negligible in commercial fortified dried milk sold in Japan. When the proposed method was applied to 5 kinds of commercial fortified dried milk, satisfactory results were obtained.View full abstractDownload PDF (1041K) -
Kiyoshi TSUKIDA, Kayoko SAIKI, Makiko SUGIURA1981 Volume 27 Issue 6 Pages 551-561
Published: 1981
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSDifferent isomerization experiments on all-trans-β-carotene (I) yielded multi-component mixtures of cis isomers. Separation and isolation of these isomers were achieved by classic adsorption chromatography on a lime column. Investigation of their 200 MHz 1H and 50.3 MHz 13C-NMR spectra led to the structural elucidation of the main cis-β-carotenes, namely the 9-monocis structure (II) for neo β-carotene U, and the 13-monocis structure (III) for neo β-carotene B. Formation of other hindered monocis (7-cis and 11-cis) and 15-monocis isomers should be carefully examined by a suitable method such as high-performance liquid chromatography.View full abstractDownload PDF (658K) -
Toshiyuki MATSUI, Shozaburo KITAOKA1981 Volume 27 Issue 6 Pages 563-572
Published: 1981
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESS“Wasanbon” sugar is handmade sugar which has been manufactured traditionally in Japan by a unique refining procedure, and is used in the making of Japanese traditional confectionary. No reports have been published on the substances responsible for the unique aroma of “Wasanbon” sugar. In this paper, the contents and compositions of the aroma in “Wasanbon” sugar and refinery final molasses are reported as studied by column chromatography, gas chromatography, mass spectrometry and sensory evaluation. The samples are the first press-off molasses (“Ara-mitsu” molasses), refinery final molasses, “Shiroshita” sugar (prerefined sugar) and “Wasanbon” sugar.
The summarized results are as follows: In the acidic fraction, the aroma of 3-phenylpropionic acid is similar to the stored aroma of “Wasanbon” sugar, whereas the aroma of its methyl ester was not similar to that aroma. Although aroma contents of the weakly acidic fraction in “Wasanbon” sugar and refinery final molasses are 8.5 to 8.7% of those of the acidic fraction, and their main components are cyclotene and maltol, which are formed by thermal degradation of sugar. These components show a higher preference than other weakly acidic fraction aromas, by a paired preference test. Cyclotene and maltol increased about 3.7 and 1.5 times, respectively, by the heating of “Shiroshita” sugar.View full abstractDownload PDF (837K) -
Toshiyuki MATSUI1981 Volume 27 Issue 6 Pages 573-582
Published: 1981
Released on J-STAGE: June 15, 2009
JOURNAL FREE ACCESSLittle is known about the greening reaction of chlorogenic acid with amino acid in alkaline conditions. The isolation and characterization of the pigment were attempted by using ethyl Caffeate and methylamine instead of chlorogenic acid and amino acid. The green compounds were purified by using Sephadex G-10 and thin layer chromatography, and were analyzed by ESR and C-13 NMR.
The ESR spectrum of F3 (corresponding to purified pigment) in a liquid state showed that the structure of the C-OH moiety seemed to give either the quinone type or its polymerized form. The ESR spectrum of F3 in a solid state showed that the g-value of this fraction was 2.003. On the basis of the C-13 NMR spectrum, the signals assigned to two carbons (C-1'' and C-2'') of the alcohol moiety of ethyl caffeate were observed clearly in F3. Considering they chemical shift of C-1 in ethyl caffeate, the C-1 in F3 could exist as -C=N-CH3. F3 seemed to be a precursor of the greening compound.View full abstractDownload PDF (744K) -
Yasuo NAGATA, Kazunari TANAKA, Michihiro SUGANO1981 Volume 27 Issue 6 Pages 583-593
Published: 1981
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSRats and mice were fed soy-bean protein or casein diets for 10 and 50 weeks, respectively, during which terms their serum cholesterol levels were analyzed periodically. Rats fed high-cholesterol diets containing soy protein or the amino acid mixture simulating soy protein produced lower levels of serum cholesterol throughout the experiments, as compared with those on the corresponding casein-type diets. Feeding soy protein resulted in a significant decrease in serum apoA-I and apoB, but the relative concentration of high-density lipoprotein-cholesterol was kept at the higher level. The concentration of liver cholesterol was also lower in rats fed the plant protein. In mice fed a cholesterol-free diet, the cholesterol-lowering effect of soy protein was noticeable at an early stage of the feeding periods, by 20 weeks. The extent of lipid peroxidation in rats and mice determined as TBA-reactive substances in serum was found to be the same when protein diets were given, while it was significantly higher when an amino acid mixture of the soy protein type was fed to rats. The results confirm that soy protein exhibits its hypocholesterolemic effect even when a diet rich in cholesterol is fed. The cholesterol-lowering effect of soy proteins appears to be a phenomenon common to rodents.View full abstractDownload PDF (1000K) -
Mampei SUZUKI, Hiromichi OKUDA1981 Volume 27 Issue 6 Pages 595-598
Published: 1981
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSATP citrate lyase [EC. 4.1.3.8] activity in human adipose tissue was assayed. The activity was considerably higher in tissue from patients who had been supplied with nutrients only by intravenous injection than that from patients who had orally taken a meal and then fasted overnight. These results suggest that ATP citrate lyase activity in human adipose tissue is greatly influenced by the nutritional status.View full abstractDownload PDF (370K) -
Shigeru ONO, Hiroko HIRANO, Susumu HAMAJIMA, Saburo HORIUCHI1981 Volume 27 Issue 6 Pages 599-604
Published: 1981
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSDownload PDF (461K)
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