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Volume 16 (1994)
- Issue 4 Pages 263-
- Issue 3 Pages 201-
- Issue 2 Pages 139-
- Issue 1 Pages 1-
Volume 16, Issue 4
Displaying 1-6 of 6 articles from this issue
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Hiroyuki KIKUHARA, Midori OGAWA, Hiroshi MIYAMOTO, Yoshihiko NIKAIDO, ...Article type: Original
1994 Volume 16 Issue 4 Pages 263-275
Published: December 01, 1994
Released on J-STAGE: April 11, 2017
JOURNAL FREE ACCESSIt has been shown that Legionella pneumophila proliferates intracellularly in more than ten species of protozoa, but the fate of the bacteria in Tetrahymena thermophila has not been reported. We investigated the multiplication of L. pneumophila Philadelphia-1 strain in micronucleated T. thermophila, and the effects of temperature and numbers of the bacteria ingested by the protozoa after in vitro feeding were studied. T. thermophila preyed actively upon the bacteria. After being ingested, the fate of the bacteria was affected by both temperature and the number of bacteria ingested. When the number of ingested bacteria was 30 per protozoon, the bacteria proliferated intracellularly at 35℃. The bacteria, however, could not proliferate at 28℃ or 32℃ though they survived in the protozoa. When the ingested bacteria was 10 per protozoon, the bacteria were killed in the protozoa at all of the temperatures tested. Electron microscopic examination revealed that the protozoa ingesting the bacteria remarkably swelled and that protozoan food vacuoles which contained L. pneumophila were studded with ribosomes.View full abstractDownload PDF (1909K) -
Michiaki MOROHASHI, Susumu UENO, Kazuo TAKEDA, Masaru KAWAMURAArticle type: Original
1994 Volume 16 Issue 4 Pages 277-286
Published: December 01, 1994
Released on J-STAGE: April 11, 2017
JOURNAL FREE ACCESSChimeric proteins consisting of parts from the α-subunit of Torpedo californica (Na, K) ATPase (N) and the rabbit sarcoplasmic reticulum Ca-ATPase (C) were expressed in Xenopus oocytes by injecting the respective chimeric cRNA in combination with cRNA for the β-subunit of Torpedo (Na, K) ATPase. The chimeric protein (NCN) that consisted of the NH2-terminal and COOH-terminal one-thirds of the α-subunit of the (Na, K) ATPase and the central one-third of the Ca-ATPase was able to assemble with the β-subunit in the same fashion as the wild-type α-subunit of the (Na, K) ATPase (NNN). On the other hand, chimeric proteins in which the COOH-terminal one-third was derived from the Ca-ATPase (NNC and NCC) were unable to form stable complexes with the β-subunit. These results suggest that the COOH-terminal one-third of the (Na, K) ATPase α-subunit is required for the assembly with the β-subunit.View full abstractDownload PDF (1124K) -
Aiguo REN, Toshiteru OKUBO, Ken TAKAHASHIArticle type: Original
1994 Volume 16 Issue 4 Pages 287-299
Published: December 01, 1994
Released on J-STAGE: April 11, 2017
JOURNAL FREE ACCESSThis study examines the association of health-related worries (over cancers, diabetes, work-related stress, heart attack, obesity, general physical fitness, and/or other health conditions) and perceived health status (excellent, good, fair or poor) to the utilization of health care services for 19,139 Japanese local public service employees. Data on health-related worries and health status were obtained from a self-administered questionnaire survey in 1988 and analyzed in relation to the subsequent 12-month utilization of health care. Results showed that perceived health status was associated with the utilization for almost all medical conditions and so was worry over a specific condition with the subsequent utilization for that or a related condition. Further analysis demonstrated that a stronger association existed between worry over a condition and the subsequent utilization among those without a history of the medical condition than the association among those with the history, thus suggesting a probable causal relationship between worry over a condition and the subsequent utilization of health care services. The implication of these findings is that measures targeting the relief of an employee's health-related worries, through either health consultation or other health programs, may contribute to the reduction of an employee's health care utilization and costs.View full abstractDownload PDF (994K) -
Yoshimasa KINOSHITA, Akira YOKOTAArticle type: Technical Note
1994 Volume 16 Issue 4 Pages 301-308
Published: December 01, 1994
Released on J-STAGE: April 11, 2017
JOURNAL FREE ACCESSMagnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS) empolying the nuclear magnetic resonance (NMR) phenomenon were used as a non-invasive method for obtaining anatomical images and for studying intracellular metabolism in vivo. A SISCO SIS 200/400 MRI/MRS experimental system for small animals (4.7 tesla, 400 mm bore) was set up, and its installation was completed in October 1993. The system makes it possible to obtain excellent quality animal images and to analyze a cellular metabolism in vivo. We introduce the details of our particular system, computers, data processing and image resolution together with examples of rat and puppy images.View full abstractDownload PDF (2331K) -
Toshiaki HIGASHI, Tetsuya MIZOUE, Takashi MUTO, Yasushi FUKUWATARI, Ka ...Article type: Review
1994 Volume 16 Issue 4 Pages 309-320
Published: December 01, 1994
Released on J-STAGE: April 11, 2017
JOURNAL FREE ACCESSAs of 1989, in Japan, around 29 million (58.3 percent) of the 49 million total labor population are working for small-scale enterprises (SSEs) where less than 50 workers are employed. This rate is higher than the average of about 40 percent in the OECD nations. It can not be denied that the occupational health service (OHS) for workers in SSEs is not adequate wheh compared with those for workers engaged in large enterprises. This can be seen in terms of occupational safety and health due to financial weakness of SSEs, and/or limitation of obligation provided for by the Law, e.g.. The appointment of an occupational health physician is not necessary in SSEs. The primary administrative authority in Japan, the Ministry of Labour, has been providing various subsidy programs for OHS in SSEs, including support for health examinations, health promotion and improvement of work conditions and environment. In 1993, the Ministry of Labour established both regional and prefectural occupational health centers to provide services for workers in SSEs. The prefectural occupational health centers also provide information and consultation regarding technical skills to OHPs.View full abstractDownload PDF (828K) -
B.P. CHATTOPADHYAY, SK. Jane ALAM, S. THAKUR, P.K. GANGOPADHYAYArticle type: Report
1994 Volume 16 Issue 4 Pages 321-331
Published: December 01, 1994
Released on J-STAGE: April 11, 2017
JOURNAL FREE ACCESSVentilatory capacity of 32 control and 318 asymptomatic male jute mill workers of the Calcutta area, India was measured by Wright's Peak Flowmeter. Mean values of peak expiratory flow rate (PEFR) was 514 ± 66.41 ℓ/min in Control and 475 ± 66.02 ℓ/min and 462 ± 67.42 ℓ/min in exposed non-smoker and smoker workers respectively. The relationship of PEFR with age, height, duration of exposure and smoking habit were studied. Multiple regression techniques were used to determine the form of the relationship of age, height, exposure and smoking habit with PEFR. The findings have been compared with those of Indian and foreign authors. The possible reasons of difference includes smoking habit, occupational exposure, physical background and residence in polluted area etc.View full abstractDownload PDF (762K)
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