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Volume 10 (1988)
- Issue 4 Pages 341-
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- Issue 2 Pages 171-
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Volume 10, Issue 4
Displaying 1-12 of 12 articles from this issue
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Junko AKIYOSHI, Hiroshi NAKAMURAArticle type: Original
1988 Volume 10 Issue 4 Pages 341-356
Published: December 01, 1988
Released on J-STAGE: April 11, 2017
JOURNAL FREE ACCESSIn order to characterize the thermodynamic property of cryoimmunoglobulin which is related to the temperature-dependent solubility change in aqueous solution, the fluorescence polarization measurement of 1-dimethylaminonaphthalene-5-sulfonyl (DNS) conjugated cryoimmunoglobulin (Jir) and monoclonal immunoglobulins in various solvent conditions were comparatively carried out using the steady state excitation method by altering temperatures from nearly 10 to 40℃, and the rotational relaxation times (Ph,) for these molecules were calculated according to the Perrin-Weber's equation using the values of polarization (P). The results indicated that the Ph value of DNS-Jir (170 nsec) was almost twice that obtained by myeloma protein (90 nsec) belonging to the same subclass with Jir, but was considerably shorter than that obtained by a soluble immune complex with a molecular weight equivalent to Jir dimer. These results suggested that the molecular structure of Jir protein was thermodynamically less-flexible than that of the myeloma proteins, and that the restricted intra-molecular flexibility might be responsible for the unusual property of cryoimmunoglobulin.View full abstractDownload PDF (1111K) -
Yuji KARASAKI, Sadao GOTOH, Shigeo KUBOMURA, Ken HIGASHI, Hideyasu HIR ...Article type: Original
1988 Volume 10 Issue 4 Pages 357-363
Published: December 01, 1988
Released on J-STAGE: April 11, 2017
JOURNAL FREE ACCESSWe have determined the nucleotide sequence of 2.0 kb EcoRI segment from the clone λ FC32 of the genomic chicken fibronectin gene, which is called DNA binding domain. This segment overlapped another clone λ FC36 and contained three exons which were 16, 17 and 18. They were classified as Type Ⅲ repeat as originally shown in bovine plasma fibronectin. The average homologies of these three exons among the chicken, rat and human fibronectins in amino acid level are very high (87－98%) compared with that (79－88%) of the exons in the cell binding domain, indicating that this region is highly conservative during the evolution.View full abstractDownload PDF (493K) -
Masami MARUYAMAArticle type: Original
1988 Volume 10 Issue 4 Pages 365-371
Published: December 01, 1988
Released on J-STAGE: April 11, 2017
JOURNAL FREE ACCESSIn nursing, it is important to master accurate skills and excellent techniques. However, it is more important to master the psychological techniques that can be learned by talking and listening to patients, and by studying their expressions as well as their behaviour. When a nurse enters a patient's room, in order to provide appropriate care, she must be able to understand at once the patient's feelings s from his or her expression, or even from the first words uttered by the patient. Sheila Dainow, an English communication counselor, said that "Empathy is a mixture of listening, observation and respect". My suggestion is that nursing education should place more emphasises on the psychological aspect of patient-nurse relationship, and teach the necessary psychological techniques.View full abstractDownload PDF (490K) -
Kayoko NAKASHlMA, Naoko TAKASE, Ken HIGASHIArticle type: Original
1988 Volume 10 Issue 4 Pages 373-380
Published: December 01, 1988
Released on J-STAGE: April 11, 2017
JOURNAL FREE ACCESSMetabolic activation of environmental chemicals into ultimate mutagens using the Salmonella system by enzymes from broccoli buds (Brassica olerancea var. Italika Plenck) was investigated. The mutagenicity of the metabolites of aflatoxin B1 in the broccoli/Salmonella TA98 system was reduced to approximately 80% of control in the presence of either SKF 525A (0.25－1.0 mM) or 7,8-benzoflavone (0.01－1.0 mM), but the inhibition was less significant with metyrapone (1－10 mM). These results suggest that at least part of the metabolic activation of aflatoxin B1 by the microsomes of broccoli was catalyed by cytochrome P-450, that is, two-electron oxidation. On the other hand, the metabolic activation of benzo(a)pyrene in the broccoli/Salmonella TA104 system, which is sensitive to detect oxidative mutagen, was observed to be enhanced in the presence of arachidonic acid (0.1－0.6 mM) probably due to the production of arachidonic acid hydroperoxide. This result suggests that the peroxidase-like mechanism, that is, one-electron oxidation, is participated in the metabolic activation of benzo(a)pyrene by broccoli enzymes. Thus the activating process of environmental chemicals in the microsomes of oxidation.View full abstractDownload PDF (649K) -
Masuhisa TSUKAMOTO, Yasuo NAKAJlMA, Cheng-Kuo CHUANGArticle type: Original
1988 Volume 10 Issue 4 Pages 381-390
Published: December 01, 1988
Released on J-STAGE: April 11, 2017
JOURNAL FREE ACCESSIsozyme patterns in three taxa, Oncomelania nosophora (collected from Japan), O. formosana (from Formosa), and O. quadrasi (from the Philippines), were compared by discontinuous electrophoresis with 5% polyacrylamide gel. Among 16 enzymes examined, malate dehydrogenase (MDH), esterase (Est), and glucosephosphate isomerase (GPI) showed significantly high enzyme activities, but lactate dehydrogenase (LDH), malic enzyme (ME), xanthine dehydrogenase (XDH), hexokinase (HK), alkaline phosphatase (AIP) and asparate aminotransferase (GOT) showed very low activities under the experimental conditions used. Band mobility was highest in leucine aminopeptidase (LAP) followed by alcohol dehydrogenase (ADH), phosphoglucomutase (PGM), acid phosphatase (AcP), isocitrate dehydrogenase (lDH), AIP, GOT, HK, glucose-6-phosphate dehydrogenase (G6PD), LDH, aldehyde oxidase (Aldox), GPI, XDH, MDH, and ME. These enzymes demonstrated a single band (LAP, AcP, AIP, LDH, GPI, and ME) or 2－3 bands (ADH, Aldox, PGM, G6PD, MDH), but at least 10 isozyme bands were observed in Est. It is suggested that nosophora, formosana and quadrasi are closely related to each other as a species complex.View full abstractDownload PDF (844K) -
Toshiyuki MUTAArticle type: Original
1988 Volume 10 Issue 4 Pages 391-401
Published: December 01, 1988
Released on J-STAGE: April 11, 2017
JOURNAL FREE ACCESSThe methods of basolateral membrane isolation from rat kidney and ³H-AVP receptor assay using this basolateral membrane preparation were established. Then, the effects of analogues and drugs on AVP-receptor binding were studied. Specific ³H-AVP binding was inhibited by LVP, dDAVP and oxytocin in that order. Among the various agonistic or antagonistic drugs to AVP (fluoride, cyclophosphamide, mechlorethamine, chlorpropamide), only chlorpropamide inhibited ³H-AVP binding to the membrane. The Kd value calculated by Scatchard analysis was 1.30±0.28 nM (n＝4, M±SD), and it was increased to 2.69±0.32 nM (n＝5, M ±SD) by adding 1mM of chlorpropamide, while BMAX was unchanged. Our data show that 1 mM chlorpropamide decreases receptor affinity for AVP, and alters AVP-receptor binding in a competitive manner.View full abstractDownload PDF (942K) -
Takehiko TSUCHIYA, Toshiyuki NORIMURA, Hisao YAMAMOTO, Satoru HATAKEYA ...Article type: Original
1988 Volume 10 Issue 4 Pages 403-409
Published: December 01, 1988
Released on J-STAGE: April 11, 2017
JOURNAL FREE ACCESSThe biological effects of tritium in humans need to be clarified, because the chances of humans becoming exposed to tritium beta radiation may increase with the development of the nuclear fusion reactor. To evaluate the biological effects of tritium, it is necessary to estimate exactly the absorbed dose from the tritium beta rays in the tissue. In many reports, the absorbed dose of HTO in the tissues is estimated from the tritium content in body fluid and dose calculations are customarily based upon the water content of soft tissues, which is taken to be 0.7 to 0.8. However, these methods may not show the exact absorbed dose in the organs. In the present study, the radioactivity of the critical tissues was measured directly using a sample oxidizer and the absorbed dose was calculated from the radioactivity of tritium in the tissues. Details on the method for calculation of the absorbed dose in tissues of the mouse is shown in this report. The results suggest that the absorbed dose should be obtained from the radioactivity in the tissues.View full abstractDownload PDF (581K) -
Masataka YANO, Hajime HORI, Minoru KOGA, Isamu TANAKA, Takashi AKIYAMAArticle type: Original
1988 Volume 10 Issue 4 Pages 411-416
Published: December 01, 1988
Released on J-STAGE: April 11, 2017
JOURNAL FREE ACCESSDenitrification characteristics of biological fluidized bed reactor were studied using methanol as a carbon source. Because glass beads have been used instead of a distribution plate at the bottom of the fluidized bed, the reactor has been able to be operated 1 year. When the concentration ratio TOC to NO3- -N (TOC ratio) was less than 1.0, denitrification efficiency decreased. The axial concentration distribution of MLSS in the fluidized bed was almost constant except at the bottom. The average biofilm thickness around the fluidizing particles was 0.1 mm. After being subjected to a cultivation method and a scanning electron microscopy, denitrifying bacteria was thought to be Hyphomicrobium sp.View full abstractDownload PDF (794K) -
Masami KURIYAMA, Yasuo NAGAMOTO, Kouichi NAKATA, Tomohiro HAYAKAWA, Ak ...Article type: Case Report
1988 Volume 10 Issue 4 Pages 417-422
Published: December 01, 1988
Released on J-STAGE: April 11, 2017
JOURNAL FREE ACCESSElectrocardiogram in a 15-year-old girl showed persistent supraventricular tachycardia at rates of 130 to 140 bpm. Electrophysiological study confirmed left atrial automatic tachycardia, which was transfered to sinus rhythm by intravenous infusion of aprindine (100 mg/10 min). Therefore, aprindine (60 mg/day) was administered orally, and neither recurrence of left atrial automatic tachycardia nor side effects were observed during subsequent follow-up period of 16 months.View full abstractDownload PDF (1305K) -
Yoichi UETA, Joji TADA, Tomoko HASHIMOTO, Sadatoshi TSUJI, Yoshiyuki M ...Article type: Case Report
1988 Volume 10 Issue 4 Pages 423-426
Published: December 01, 1988
Released on J-STAGE: April 11, 2017
JOURNAL FREE ACCESSA 42-year-old woman with progressive myelopathy and mononeuritis multiplex is reported. The neurological examination on admission revealed hyperreflexia of the four extremities with pathological reflexes and moderate muscle weakness of the lower extremities with spasticity. Sensory disturbance was distributed on the areas of the bilateral lateral cutaneous femoral, the superficial peroneal and sural nerves. The antibody to HTLV-Ⅰ in the serum and cerebrospinal fluid was more than 8192× and 512×, respectively. No sensory potential was recorded in the sensory conduction study of bilateral lateral femoral cutaneous nerves. Corticosteroid therapy caused a marked improvement of the sensory and urinary disturbances and had a slight effect on the spastic gait. Our nerve conduction study found small sensory potentials with normal conduction velocities in the bilateral lateral femoral cutaneous nerves. These results suggested the presence of an axonal degeneration in the peripheral nerves in this case. There have been no reports in the literature regarding a case of HAM with mononeuritis multiplex.View full abstractDownload PDF (382K) -
Koji MORI, Naohide INOUE, Kazuya FUJISHIRO, Hajime HORI, Isamu TANAKAArticle type: Short Report
1988 Volume 10 Issue 4 Pages 427-431
Published: December 01, 1988
Released on J-STAGE: April 11, 2017
JOURNAL FREE ACCESSThe effects of chronic exposure of ethylene oxide on the testes were investigated. When rats were exposed to 500 ppm, three times a week for 13 weeks, the testes of the exposed group became remarkably atrophic and their DNA content decreased proportionally. However, plasma testosterone concentration did not significantly change. In the testes the activity of glutathione reductase decreased by 45% and glutathione-S-transferase increased by 64%, respectively. These results suggest that ethylene oxide causes a definite toxicity in the testes and the abnormality of glutathione metabolism plays an important role in its toxicity.View full abstractDownload PDF (434K) -
―A Review of Human Cases―Naohide INOUE, Kazuya FUJISHIRO, Koji MORI, Masato MATSUOKAArticle type: Review
1988 Volume 10 Issue 4 Pages 433-442
Published: December 01, 1988
Released on J-STAGE: April 11, 2017
JOURNAL FREE ACCESSSince the end of the nineteenth century, numerous cases of triorthocresyl phosphate (TOCP) poisoning due to accidental contamination of drink, food or drugs have been reported. Following the ingestion of preparations contaminated by TOCP, gastrointestinal symptoms may occur and after an interval of ten to twenty days, a well-known delayed neurotoxicity gradually develops. In general, the initial symptoms are pain and paresthesia in the lower extremities. In most cases, muscle weakness progresses rapidly developing into a striking paralysis of the lower extremities with or without an involvement of the upper extremities. Severe cases show pyramidal signs. The histopathological findings show axonal degeneration in the peripheral nerves and degenerative changes in the anterior horn cells. Degenerative change also occurs in the lateral and dorsal tracts of the spinal cord. The cardinal therapy is physical rehabilitation.View full abstractDownload PDF (753K)
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