We compared the sensitivity of new
Escherichia coli tester strains having the
TolC outer membrane transport protein mutation (
tolC strain), viz., WP2
tolC, WP2
tolC/pKM101, WP2
uvrA,tolC and WP2
uvrA,tolC/pKM101, with
E. coli strains not carrying this mutation (non-
tolC strain), i.e., WP2, WP2/pKM101, WP2
uvrA and WP2
uvrA/pKM101, by measuring the specific activity (revertants/mg) of mutagens using a preincubation method. The
tolC strains were more sensitive to polycyclic and heterocyclic compounds such as 2-aminoanthracene, 2-nitrofluorene, Glu-P-1, benzo[a]pyrene, mitomycin C, streptonigrin and doxorubicin than the non-
tolC strains. Mutagenicity of 2-nitrofluorene was not detected by non-
tolC strains WP2, WP2/pKM101 and WP2
uvrA, but was detected by their
tolC counterpart strains WP2
tolC, WP2
tolC/pKM101 and WP2
uvrA,tolC. However, these
tolC strains were less mutagenic to streptozotocin or cisplatin than that of parent strains. Mutagenicity of 9-β-D-arabinofuranoside was also not detected by the
tolC strain WP2
uvrA,tolC/pKM101, but was detected by the non-
tolC strain WP2
uvrA/pKM101. The enhancing effects of the mutagen detecting sensitivity by TolC outer membrane transport protein mutation were clearly observed with the low sensitivity strain WP2, but less clearly with the high sensitivity strain WP2
uvrA/ pKM101.
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