All issues
Volume 17 (1992)
- Issue 6 Pages 352-
- Issue 5 Pages 274-
- Issue 4 Pages 216-
- Issue 3 Pages 128-
- Issue 2 Pages 58-
- Issue 1 Pages 2-
Volume 17, Issue 2
Displaying 1-8 of 8 articles from this issue
- |<
- <
- 1
- >
- >|
-
Takanobu Sugo, Kyoichi Saito1992 Volume 17 Issue 2 Pages 58-66
Published: March 01, 1992
Released on J-STAGE: March 04, 2011
JOURNAL FREE ACCESSMembrane-based affinity chromatography has advantages over conventional bead-based chromatography for the following two reasons : (1) the module charged with hollow fibers requires a much lower operating pressure than a bead-packed bed ; and (2) as the biomolecule can be transported by convection to the ligand immobilized on the inner surface of the microporous membrane, faster adsorption onto the affinity membrane can be attained. Hollow-fiber affinity membranes containing phenylalanine and tryptophan as ligands were prepared by the radiation-induced grafting of glycidyl methacrylate onto a porous polyethylene hollow fiber, followed by coupling of the epoxide group produced with Phe and Trp. The remaining epoxide group is quantitatively converted into a diol group. The diol group renders the polymer surface hydrophilic and prevents nonselective adsorption. To evaluate the adsorption behavior of the membrane, the gamma-globulin-containing buffer solution was permeated from the inside to the outside of the Trp-containing hollow-fiber affinity membrane. The breakthrough curves as a function of effluent volume coincided irrespective of the flow-rate, i. e., the residence time of the solution across the membrane, as a result of negligible diffusional mass transfer resistance.View full abstractDownload PDF (1163K) -
Tamotsu Taketomi1992 Volume 17 Issue 2 Pages 67-77
Published: March 01, 1992
Released on J-STAGE: March 04, 2011
JOURNAL FREE ACCESSGeneral review on the recent progress of glycosphingolipids was described both in various kinds of blood cells. and in the nervous tissues. First, the cell membrane glycosphingolipids were summarized and discussed as important markers for the maturation and differentiation from immature blood cells to mature myeloid and lymphoid cells, erythrocytes and platelets, as specific ligands to some selectins for cell-cell recognition and as roles of immunological responses.
Second, the biological significance of myelin lipids, particularly cerebroside and sulfatide, changes of neuronal gangliosides in the development of neuronal cells, abnormal gangliosides in the brain tumors and biological activities of gangliosides were mentioned.View full abstractDownload PDF (1441K) -
Katsumori Nakura, Chiyoshi Kamizawa, Masaji Matsuda, Hitoshi Masuda1992 Volume 17 Issue 2 Pages 78-84
Published: March 01, 1992
Released on J-STAGE: March 04, 2011
JOURNAL FREE ACCESSA heat and solvent resistant membrane was prepared by the casting method with poly (p-phenyleneterephthalamide) (PPTA) as a membrane material.
The solvent of PPTA was concentrated sulfuric acid. Some organic solvents were employed as the gelating agents to control the molecular cut offs of the membranes.
The addition of inorganic salts such as H2SO4, to the casting solution was also effective to improve the membrane performance. The membrane was stable in almost all organic solvents and in boiled water.View full abstractDownload PDF (1161K) -
Katsumori Nakura, Chiyoshi Kamizawa, Masaji Matsuda, Hitoshi Masuda1992 Volume 17 Issue 2 Pages 85-90
Published: March 01, 1992
Released on J-STAGE: March 04, 2011
JOURNAL FREE ACCESSA membrane for ultrafiltration was prepared by the casting method with poly (etheretherketone) (PEEK) as a membrane material.
PEEK was easily sulfonated in the concentrated sulfuric acid and the ion exchange of capacity of PEEK was 1.6-1.8 meq/g at most.
Sulfonated PEEK was easily dissolved in many organic solvents and N-methyl-2-pyrrolidone was chosen as a solvent for the membrane preparation.
To control the molecular cutoffs of the membranes, NaCl solutions of various concentrations were employed as the gelating solutions.
The membranes could reject inorganic salts such as Na2SO4, and could separate some amino acids, though the molecular cutoff of the membrane was 50, 000 or so.View full abstractDownload PDF (1497K) -
Shinsuke Takegami, Hideki Yamada1992 Volume 17 Issue 2 Pages 91-96
Published: March 01, 1992
Released on J-STAGE: March 04, 2011
JOURNAL FREE ACCESSThe evaluation methods of pervaporation performance were studied using direct measurement of permeate vapor by gas chromatography (direct gas chromatographic method) and cold trap of permeate vapor (cold trap method).
The membrane performances of ethanol-water separation were compared by two method above mentioned. As the results, it was found that the separation factor of ethanol to water in the EtOH-H2O pervaporation through poly (dimethylsiloxane) [poly (DMS)] and styrene-DMS graft copolymer membranes by direct gas chromatographic method became larger than by cold trap method and the permeate rate became smaller than by cold trap method, especially in the case that the sampling tube of permeate vapor was small. It is confirmed that these results are caused by the increase of permeate side pressure and condensation of water rather than ethanol, because the condensation temperature of water is lower than that of ethanol.View full abstractDownload PDF (616K) -
J. Y. LAI, C. T. CHENG1992 Volume 17 Issue 2 Pages 97-103
Published: March 01, 1992
Released on J-STAGE: March 04, 2011
JOURNAL FREE ACCESSThe effect of adding certain inorganic salts mainly containing transition metal chlorides (hexahydrate) to the casting solutions of nylon 4 is to swell the nylon 4 membranes and to improve the permeability of NaCl solute for the nylon 4 dialysis membranes. Swelling is affected by the formation of complexes involving the inorganic salts and the amide group of the nylon 4 polymer substrate. The morphologies using scanning electron microscopy (SEM) were studied. The permeability of NaCl and the water content evaluated as a function of the amount of inorganic salts : FeCl3·6H2O, CoCl2·6H2O, NiCl2·6H2O, CuCl2·6H2O and AlCl3·6H2O added. Especially, the best permeability of NaCl is the nylon 4 membrane by adding the different amount of aluminum chloride. The nylon 4 membranes were analyzed by UV-Visible and FT-IR spectroscopy, to investigate the formation of complexes between the salts and the nylon 4 polymer substrate.View full abstractDownload PDF (1605K) -
Mamoru Nango1992 Volume 17 Issue 2 Pages 105-114
Published: March 01, 1992
Released on J-STAGE: March 04, 2011
JOURNAL FREE ACCESSPhospholipid-linked porphyrins separated by spacer methylene groups (PE-Cn-TTP) were synthesized. Energy transfer from zinc porphyrin to the porphyrin anchored onto a lipid bilayer showed that incorporation of the porphyrin into the lipid membrane of either egg yolk phosphatidylcholine (PC) or dipalmitoyl-phosphatidylcholine (DPPC) was much higher than non-linked porphyrin, tetratolylporphyrin (TTP) and our previously reported polyethylenimine-linked porphyrins. Manganese complexes of PE-Cn-TTP catalyzed transmembrane electron transport, depending not only by the length of separated spacer methylene groups between porphyrin and phospholipid but also by the fluidity of the lipid. The rate was significantly affected due to the presence of imidazole derivative in the lipid bilayer.View full abstractDownload PDF (932K) -
[in Japanese]1992 Volume 17 Issue 2 Pages 115-120
Published: March 01, 1992
Released on J-STAGE: March 04, 2011
JOURNAL FREE ACCESSDownload PDF (742K)
- |<
- <
- 1
- >
- >|