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Volume 21 (1975)
- Issue 6 Pages 387-
- Issue 5 Pages 317-
- Issue 4 Pages 237-
- Issue 3 Pages 151-
- Issue 2 Pages 73-
- Issue 1 Pages 1-
Predecessor
Volume 21, Issue 2
Displaying 1-13 of 13 articles from this issue
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Kenji FUKUZAWA, Masao SATO1975 Volume 21 Issue 2 Pages 73-78
Published: 1975
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESS12-Keto oleic acid, possibly one of the oxidation products of long-chain, unsaturated fatty acids, was added to the feed of weanling male rats at the 1% level. Their growth curves, tissue weights, plasma alkaline phosphatase, GOT, and GPT activities, and plasma and liver lipid (cholesterol, triglyceride and phospholipid) levels were investigated and compared with those of weanlings fed a vitamin E deficient diet. Both the diet containing 12-keto oleic acid and the diet deficient in vitamin E decreased the growth rate of body weight and tissue weight, and increased the liver triglyceride and cholesterol levels. Parallel with these, increased hemolysis and stimulation of lipid peroxidation and fluorescent production in the liver homogenate were observed. Elevated plasma alkaline phosphatase and GOT activities which may be considered to be due to a functional disorder of the liver were also observed.View full abstractDownload PDF (641K) -
Kenji FUKUZAWA, Masao SATO1975 Volume 21 Issue 2 Pages 79-88
Published: 1975
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESS12-Keto oleic acid is generally considered to be a secondary degradation product of peroxides produced by lipid peroxidation. The acid was added to a mouse liver homogenate or to a bovine serum albumin and its influences on lipid peroxidation and fluorescent production were investigated and compared with other acids. Just as with vitamin E deficiency, 12-keto oleic acid was shown to increase lipid peroxide formation and fluorescence production directly and indirectly. The increase of lipid peroxide formation was caused directly through the increase of the free radical production, and indirectly through change of the biomembrane structure. The fluorescence production increase was caused directly by reaction of the 12-keto oleic acid itself, and indirectly by acceleration of the lipid peroxidation.View full abstractDownload PDF (907K) -
P. K. PAUL, P. N. DUTTAGUPTA1975 Volume 21 Issue 2 Pages 89-101
Published: 1975
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSAn acute dose of biotin (10mg/100g body weight) in two subcutaneous injections when given to a rat on day 1 and 2 of pregnancy, caused resorption of fetuses and placentae. Pregnancies under such biotin-treated conditions were maintained by continued estrogen or progesterone therapy. Biotin-treated pregnant rats failed to maintain normal levels of uterine weight, glycogen and protein as well as hepatic protein concomitantly with the loss of pregnancy. Estrogen therapy under such conditions improved all the parameters in these organs including the placenta, but progesterone therapy did not. Glucose-6-phosphate dehydrogenase (G-6-PD) activity in ovary, adrenal, liver and uterus was also reduced following biotin-induced loss of pregnancy which had been improved by estrogen or progesterone therapy. Nevertheless, estrogen was superior to progesterone in stimulating the enzyme activity in these organs excepting the adrenal. As far as the tissue response to biotin, estrogen or progesterone in the nonpregnant rat is concerned, biotin and progesterone exerted a suppressing effect on uterine glycogen and protein and also on liver protein, while estrogen stimulated them. Similarly, biotin and progesterone adversely affected G-6-PD activity in all the organs studied except the liver and adrenal. Estrogen stimulated the enzyme activity in all these organs but adrenal. The study suggests that the primary reason for an acute dose of biotin-induced loss of pregnancy is blockage of estrogen production, which probably regulates endogenous progesterone secretion. The associated metabolic derangements are probably secondary to estrogen deficiency and are discussed.View full abstractDownload PDF (1401K) -
Hisateru MITSUDA, Yukio TAKII, Kimikazu IWAMI, Kyoden YASUMOTO1975 Volume 21 Issue 2 Pages 103-115
Published: 1975
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSThiamine pyrophosphokinase was purified about 8, 000-fold from extracts of parsely leaves. The enzyme, as prepared, was homogenous on polyacrylamide gel electrophoresis. The molecular weight of the enzyme, estimated by gel filtration with Sephadex G-150, was approximately 30, 000. In 0.05M Tris-HCl, the enzymic activity showed a pH optimum over a range of 8 to 9. A least squares analyses of Lineweaver-Burk and Hofstee plots gave Km values of 0.8mM and 0.15μM for ATP and thiamine, respectively. Thiamine homologues and analogues so far tested, except for cetyl thiamine, were all inactive as substrates. The enzyme was specific for ATP and Mg++, although to a lesser extent a combination with other ribonucleoside triphosphates or divalent cations could replace them. SH reagents, such as PCMB, NEM and iodoacetamide, were potent inhibitors of the enzyme. The inhibition was prevented by the addition of dithiothreitol. Inorganic pyrophosphate exhibited striking inhibition. TMP could not replace thiamine as the substrate, whereas it inhibited the TPP formation from thiamine. These findings are consistent with the views that TMP is not directly converted to TPP but after being dephosphorylated by the action of a monoesterase, thiamine is pyrophosphorylated with ATP by thiamine pyrophosphokinase (EC 2.7.6.2) to form TPP and thus give a clear evidence regarding the mechanism of TPP formation in plant tissues.View full abstractDownload PDF (1218K) -
Kyoden YASUMOTO, Kenjiro TADERA, Hideaki TSUJI, Hisatern MITSUDA1975 Volume 21 Issue 2 Pages 117-127
Published: 1975
Released on J-STAGE: June 15, 2009
JOURNAL FREE ACCESSA simple system for semi-automatic analysis of pyridoxal, pyridoxine and pyridoxamine has been developed using diazide of 5-chloroaniline 2, 4-disulfonyl chloride as a color-producing reagent in combination with desoxypyridoxine as an internal standard. It employs Aminex A-5 column, 10×0.9∅cm, as an adsorbent. Adsorbates were eluted successively with 3 discrete phosphate buffers (0.4N Nat). The effluent is mixed continuously with capillary streams of the diazide reagent and of sodium acetate. The mixture, maintained at 65°C in a heating bath, then passes through a spiral of Teflon tubing with a residence time of 2min. Characteristic orange colored products formed by a diazo coupling reaction are continuously monitored at 440nm in a flow photometer. The individual peaks on the recorded chromatogram are manually integrated by a conventional HW method. The elution position and recovery of desoxypyridoxine permits correction for sensitivity changes or mechanical losses which might occur during a series of analyses. The analyzer system described allow quantitation from 2 to 25μg of pyridoxal, pyridoxine and pyridoxamine in a single sample within 2 hr and with a precision of 100±4%. It is also found suitable as a routine procedure for the analysis of varied biological samples.View full abstractDownload PDF (1291K) -
Katsunori OGASA, Morio KUBOYAMA, Isao KIYOSAWA, Takashi SUZUKI, Mayumi ...1975 Volume 21 Issue 2 Pages 129-135
Published: 1975
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSThe gaschromatographic method was used for the quantitative analyses of inositol in milk. The content of inositol in human and cow's milk at the different lactation periods was determined. The content of total myoinositol in human milk was 32.7±15.2mg/100ml in colostrum, 17.8±1.9mg/100ml in transitional milk, and 14.9±3.1mg/100ml in mature milk. In cow's milk, it was 10.6±1.0, 7.0±1.1, and 4.1±1.0mg/100ml, respectively. These values were very similar to those obtained by the microbiological method. The presence of lipid-bound myoinositol in both kinds of milk was confirmed and the content was 0.22±0.09mg/100ml in human milk and 0.36±0.10mg/100ml in cow's milk. A small amount of scylloinositol was found in both human and cow's milk, while dextroinositol was not found in either.View full abstractDownload PDF (713K) -
Teruo MIYAZAWA, Seisuke ITO, Yasuhiko FUJINO1975 Volume 21 Issue 2 Pages 137-142
Published: 1975
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSPea seeds were shown to contain ten kinds of neutral lipids, among which triglyceride, free sterol and sterolester are the main components and diglyceride, monoglyceride, free fatty acid, wax and some pigments are the minor ones. The major component fatty acids in the glycerides and the free fatty acid are C18:2, C18:1 and C16:0. The positional distribution of fatty acids in triglyceride is such that, position 1 and 3, especially the former, abound in saturated fatty acids, and position 2 is filled almost entirely with unsaturated fatty acids.View full abstractDownload PDF (555K) -
Yasuo IWANAMI, Miki AKINO1975 Volume 21 Issue 2 Pages 143-145
Published: 1975
Released on J-STAGE: April 28, 2009
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Kiyoshi TSUKIDA, Kayoko SAIKI, Masayoshi ITO, Ikuko TOMOFUJI, Mikiko O ...1975 Volume 21 Issue 2 Pages 147-149
Published: 1975
Released on J-STAGE: April 28, 2009
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1975 Volume 21 Issue 2 Pages e1a
Published: 1975
Released on J-STAGE: April 28, 2009
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1975 Volume 21 Issue 2 Pages e1b
Published: 1975
Released on J-STAGE: April 28, 2009
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1975 Volume 21 Issue 2 Pages e1c
Published: 1975
Released on J-STAGE: April 28, 2009
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1975 Volume 21 Issue 2 Pages e1d
Published: 1975
Released on J-STAGE: April 28, 2009
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