9, 12-Hexadecadienoic acid (16:2
n-4), present in small amounts in fish oils as a naturally occurring unique fatty acid, was incorporated into the phospholipids in rat liver BRL-3A cells to a similar extent as linoleic acid (18:2
n-6). 11, 14-Octadecadienoic acid (18:2
n-4) and 8, 11, 14-octadecatrienoic acid (18:3
n-4) were detected in the cellular lipids of BRL-3A cells when incubated in a medium supplemented with 16:2
n-4 methyl ester. The cellular levels of these acids increased in parallel with 16:2
n-4 methyl ester added to the medium. These compounds were probably formed through conversion from 16:2
n-4 to 16:3
n-4 by d 6 desaturation, and then 18:3
n-4 was produced by elongation, and part of the surplus 16:2
n-4, not desaturated to 16:3
n-4, elongated to 18:2
n-4. These results suggested that 16:2
n-4 was desaturated by d6 desaturase in vitro. It was also shown that 16:2
n-4 inhibited arachidonic acid synthesis from exogenous linoleic acid in BRL-3A cells as efficiently as a-linolenic acid (18:3
n-3).
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