Journal of Environmental Conservation Engineering
Online ISSN : 1882-8590
Print ISSN : 0388-9459
ISSN-L : 0388-9459
Volume 43, Issue 5
Displaying 1-2 of 2 articles from this issue
  • : Batch Test Evaluation
    Masashi TAKEKAWA, Yuki NAKAGAWA, Keiji TAKAI, Satoshi SODA, Michihiko ...
    2014 Volume 43 Issue 5 Pages 293-300
    Published: May 20, 2014
    Released on J-STAGE: June 02, 2014
    JOURNAL FREE ACCESS
    In order to investigate the possibility of simultaneous anammox and heterotrophic denitrification(SAD)as a novel nitrogen removal process, batch experiments for nitrogen removal from synthetic wastewater containing acetate, nitrate, and ammonium were conducted using a mixture of activated sludge and anammox sludge. It is suggested that anammox bacteria removed ammonium and nitrite reductively generated from nitrate by heterotrophic denitrifiers existing in the activated sludge. In the batch experiments with the acetate-C/nitrate-N ratios over 0.5, total nitrogen removal was higher than 90% by occurrence of SAD. These results suggest that the SAD process can efficiently remove nitrogen under a wide range of the C/N concentrations, especially under low C/N ratios.
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  • Taketoshi UZAWA, Youbin MOON
    2014 Volume 43 Issue 5 Pages 286-292
    Published: May 20, 2014
    Released on J-STAGE: June 02, 2014
    JOURNAL FREE ACCESS
    DNA detection methods by staining agarose gels after electrophoresis were evaluated to find safer ones suitable for experiment on environmental education. Detection method using dye and visible rays is thought to be safer than the method using ethidium bromide and ultraviolet rays. Three methods using dye and visible rays: staining by(a)ViewaBlue Stain,( b)stains-all, or( c)indoine blue and methyl orange, were compared with detection method using ethidium bromide. These detection methods were less sensitive than that using ethidium bromide. In the methods using dye and visible rays, stains-all method was the most sensitive.Moreover, costs of each methods were estimated for educational use.
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