Very few informations with regard to the insecticide resistance of medical flies are available other than the house fly. In this paper, γ-BHC resistance in a flesh fly, Boettcherisca peregrina, which is a main pest in the privy-pot of our native-type lavatories, was reported. The flies collected from Yushima, Tokyo, in 1962, and Ohmiya, Saitama Prefecture, in 1964 were tested after being reared in laboratory and compared with a susceptible strain maintained in our insectarium since 1956. For detecting the resistance of the larvae, the last instar larvae were tested under the author's "dilution contact method" in which the larvae were exposed to a thin layer of desired concentrations of insecticide emulsion for 48 hours and the mortality was counted. The adults were assessed by topical application test. The LC-50 values in the larval tests were 19ppm in susceptible strain, 125ppm in Yushima colony and 500ppm in Ohmiya colony. Both colonies, Yushima and Ohmiya, were, consequently, fairly resistant to γ-BHC, showing 6.6 and 26.3 folds respectively as resistance ratio. Their resistance seem to be caused by frequent spraying with γ-BHC emulsion over the privy-pots. The LD-50 values in the adult flies were 0.08μg per female fly in susceptible strain, 0.1μg in Yushima and 0.26μg in Ohmiya. Though the latter colony is certainly resistant to γ-BHC, the resistant level is not so remarkable as shown of their larval stage. It was also confirmed, using fenthion emulsion to the larvae, that the γ-BHC resistant Ohmiya colony is still susceptible to it.
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