Many studies on the substances that control the insect metamorphosis have done recently and Butenandt has successed to obtein the "verpuppungshormon" in the crystall form from silkworm pupa in 1954. However, the progress of the body constituents in insect tissue during metamorphosis is not yet enoughly explained. In this paper the investigations about the relation between tissue prctein and free aminoacid during metamorphosis of Cu'ex pipiens were carried out to explain certain parts of above question. The result is shown on the Fig. I. The results can be summarized as follows : Free amino-acids in tissues are much aboundant in 3 and 4-instar larvae and they decrease rapidly just after pupation. Although the free amino-acids content increases slightly in pupal stage, it is relatively constant during this stage and similarly it does not show any notable changes after emergence. On the other hand the tissue protein is most scant in 3-instar larvae and it increases rapidly in accordance with their developement to the 4-instar larvae and pupation. During pupal stage the tissue protein content is constant generally and it is also similar after emergence. Such rapid decrease of free amino acids and the rapid increase of protein in tissues during pupation suggest the vigorous histogenesis for producing the new tissues in this stage. And during the pupal stage contents of free amino-acids and protein are both constant in general. This constancy seems to show the histolysis and histogenesis progress in this stage relatively constant or may utilize chiefly the free amino acids during pupation and glycogen and fat substances during pupal stage. Quality of free amino acids in each stages of 4-instar larvae, pupae (initial stadium), pupae (end stadium), adults (initial stadium) are shown on Table I, estimated by paper chromatography. It shows the presence of : in 4 instar larvae 18 free amino-acids (α-alanine, glutamic acid, leucine & isoleucine, proline, histidine, serine, valine, methionine, lysine, arginine, aspartic acid, glycine, tyrosine, phenylalanine, cystine, hydroxyglutamic acid, methionine sulfoxide), in pupae of initial stadium 11 (α-alanine, glutamic acid, leucine & isoleucine, histidine, valine, elysin, arginine, aspartic acid, glycine, tyrosine), in pupae of end stadium 8 (α-alanine, glutamic acid, leucine & isoleucine, valine, arginine, glycine, tyrosine), and in adults 10 (α-alanine, glutamic acid, leucine & isoleucine, proline, histidine, valine, lysine, arginine, glycine) respectively. These results seem to indicate also that the free amino acids content in 4-instar larvae is much predominant as compared with them in the pupal or adult stages.

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Questioned as differently among species of the Culex pipiens strains had for long been repeated or scrutinized, but in the year of 1951 the questionables as such became scrutinized by Mattingly et al. Viewing from the world map of districts, it proved to cross with each other between pipiens in the North Temperate and fatigans (quinquefusciatus) in the South, so some of the intermediate type of both species as above mentioned were known as C. p. var. pallens being able to see in Japan and China which can be made unautogenous on the one hand, and as C. molestus together with C. comitatus and other being distributed in various parts of Europe and America which can be made autogenous on the others. These were scrutinized to have been different to that of Culex pipiens strains. Since C. p. var. pallens on the hand to be seen in Japan was made known as a subspecies by Edwards in 1921, the autogenous species of this strains has not been reported in Japan, but it was possible to find out at the subway or basement of ironcon-creted buildings in Osaka City and so I intend to make a bid for some data acquired from morphological and ecological point of view on the foregoing question of the difference. I collected this autogenous pipiens got from a water-lodged basement in the form of larvae and fed them in our laboratory and completed the third generation in succession for experimentation. In addition to that, I conducted to study of whether or not there has been different among C. p. var. pallens, C. molestus and others morphologically and ecologically in their comparison. Compared to pallens as morphological, the species (autogenous) in the form of adult was a little smaller in size, body is pale brown in color, palps short, indistinctive of yellowish fleck on each one of bases of the abdominal segments, not having of the white belt in the respective end of femur and tibia of hind legs, the ratio of length of cell R_2, M_2 in its wing petiole is large in size, male hypopygial phallosome flatly more or less, in larva length of siphon long, siphonalhairs, pectens, and lateral scales in number more than that of pallens and these characters have been suggested as an intermediate form between pipiens and pallens, anal gill is comparatively shorter. Morphological type of view in both of the adult and larva is appeared near to C. moleslus, but as there are many variations in each one of population, it is seen to be involved in the range of pallens. The adult, ecologically speaking, under-ground spot to live can be active all the year round in case of more than 70 per cent of humidity, around 28 Centigrade in Summer season and 14C. in Winter but it makes no hibernation at all. In Winter, it was seen that the adult species could do well to bite and to suck blood in natural state of affairs and larvae of this strains were also seen in such clean water-lodged place as being comprised in its water quality, with a lot of chlorine, which indicated of alkaline pH 9.5 in the highest. To speak of the water temperature there was little change of phenomonon throughout the year but some 12 Centigrade in Winter and some 28 C. in Summer. In respect to an influence of the salinity, it was less changeable in comparison with pallens as the result of having done experiments on the change of the consistency in divers manner by function of the anal gill which is regarded as its taking-in organ and showed a value being between pipiens and pallens. As for the fnfluence of the larvae towards temperature conditions, they were comparatively fast in its growth at a low point of thermometer and slow in the high point, but more than 30 Centigrade brought them to a standstill on the way of growth and to death without pupalization in the end. Even adult of this strains in the case of over 30 Centigrade went down its reproductive function and was known near to pallens judging from the morphological side of view. Although

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Field surveys were made on the ectoparasite of the wood mice at KARAO-valley, HIROKAWA-cho, ARITA-gun, WAKAYAMA-ken, JAPAN, through a year from July 1954 to June 1955. In the present paper, as a part of these surveys, we report on the seasonal fluctuations of the population of chigger mites and their species complex. The results are as follows; 1. Throughout these surveys, 59 individuals of mice as host and 5281 individuals of chiggers of 9 different species as ectoparasites are captured. 2. We can divide following three types in the seasonal fluctuations of their populations which are represented by the mean numbers of the collected chiggers per one host. A. During the all seasons can be caught, but few. Trombicula kansai, Gahrliepia suduski. B. The mode of population appeares in November or December. T. kitasatoi, T. pallida, T. miyajimai. T. japonica. C. The mode of population appears in January or February. T. fuji, T. kochiensis, T. daisen. 3. Chigger fauna at the different area does not differ significantly. 4. Average number of the rankings of abundance of each species on the whole individuals of captured mice (Ranking index), does not always change in parallel with the mean of population density of each species. 5. By the difference of fluctuation of the rankings of abundance of each chigger, the species complex of mites on each mouse can be divided into following three types; A. Standard deviation of the ranking is always smaller than 1 T. fuji, T. kochiensis. B. Standard deviation of the ranking varies from 0 to 2 T. kitasatoi, T. pallida, G. suduski. C. Standard deviation of the ranking is always larger than 1 T. daisen, T. kansai, T. miyajimai, T. japonica. 6. The correlation between the body weight of the host and collected number of the chiggers is highly significant, and its correlation coefficient is +0.602.

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[in Japanese]

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[in Japanese]

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