Altosid 10F[○!R], a slow-release formulation which contains 10% methoprene, was tested on the larvae and pupae of 8 mosquito species collected from Okinawajima, Japan : Culex quinque-fasciatus Say, Cx. tritaeniorhynchus Giles, Cx. fuscanus Wiedemann, Aedes albopictus (Skuse), Ae. riversi Bohart and Ingram, Ae. daitensis Miyagi and Toma, Ae. iriomotensis Tanaka and Mizusawa, and Armigeres subalbatus (Coquillett). The residual effect of methoprene on Ae. albopictus was tested under 4 different conditions in the laboratory. The IC_<50> value for 1st instar larvae was 0.0017ppm against Ae. iriomotensis, 0.0120ppm against Ae. albopictus, 0.0176ppm against Ae. riversi, 0.0374ppm against Cx. quinquefasciatus, 0.0466ppm against Cx. tritaeniorhynchus, 0.0743ppm against Ae. daitensis, 0.0976ppm against Cx. fuscanus, and 14.9352ppm against Ar. subalbatus. The IC_<50> value for 4th instar larvae was 0.00006ppm against Ae. iriomotensis, 0.0009ppm against Cx. fuscanus and Ae. albopictus, 0.0012ppm against Cx. tritaeniorhynchus, 0.0013ppm against Cx. quinquefasciatus, and 1.2819ppm against Ar. subalbatus. The effectiveness of methoprene against Ae. albopictus persisted for a 7-month period when it was applied to the containers and left in a dry state in the laboratory. When methoprene was applied and left in the aqueous state, the percentage of inhibition of adult emergence was high, 85.5% for a 3-month period, but dropped to 39.5% after a 4-month period. When the containers were subjected to alternating aqueous and dry states, the inhibition of adult emergence was intermediate between the respective dry and aqueous conditions. When a container treated with methoprene was used successively for mosquito rearing, the effect of methoprene decreased within a short period, the inhibition rate being 36.5% after a 1-month period.
View full abstract