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Volume 23 (1977)
- Issue 6 Pages 475-
- Issue 5 Pages 377-
- Issue 4 Pages 265-
- Issue 3 Pages 169-
- Issue 2 Pages 53-
- Issue 1 Pages 1-
Predecessor
Volume 23, Issue 3
Displaying 1-11 of 11 articles from this issue
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Osamu IGARASHI1977 Volume 23 Issue 3 Pages 169-178
Published: 1977
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSN-Methyl-2, 2, 7, 8-tetramethyl-6-amino-chroman, a model compound of N-methyl-γ-tocopheramine, was submitted to an oxidation study with alkaline potassium ferricyanide. As oxidative products, 2, 2, 7, 8-tetramethyl-6-amino-chroman and dimeric (VI) were identified. Also, a trace of compound C which might be a azochroman was detected. It was concluded that an N radical formation is necessary to take the role of a biological antioxidant of N-methyl-γ-tocopheramine.View full abstractDownload PDF (809K) -
Tadashi KAMIKUBO, Ken SASAKI, Mitsunori HAYASHI1977 Volume 23 Issue 3 Pages 179-185
Published: 1977
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSCo-free corrinoids (CFCs) were found in the cells of Rhodopseudomonas spheroides grown without cobalt salt. A predominant fraction of three CFCs on paper-electrophoregram was further purified, and investigated physico-chemically as well as microbiologically in comparison with that isolated by TOOHEY from Chromatium. The electrophoretic as well as spectrophotometric patterns of CFC from Rh. spheroides were partly different from those of Chromatium CFC. The former CFC was a competitive inhibitor of cyanocobalamin in Ochromonas malhamensis. The findings obtained indicate that the CFC from Rh. spheroides resembles that from Chromatium in chemical structure with some difference, and that the compound has an anti-vitamin B12 activity in O. malhamensis.View full abstractDownload PDF (682K) -
Fumio SHIMURA, Sachiko MORIUCHI, Norimasa HOSOYA1977 Volume 23 Issue 3 Pages 187-200
Published: 1977
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSCytosol binding protein for 1α, 25-(OH)2D3 and 25-OHD3 was demonstrated in rat intestinal mucosa using sucrose density gradient ultracentrifugation analysis, Sephadex G-200 column chromatography and polyacrylamide disc gel electrophoresis. The binding protein has a sedimentation constant of 5-6S, a molecular weight of 100, 000-120, 000 daltons and a mobility in electrophoresis of Rf 0.42. Further analysis of binding behavior by DEAE-cellulose filter assays revealed that the binding protein had a higher affinity for 25-OHD3 than for 1α, 25-(OH)2D3, and that 1α, 25-(OH)2D3 competed with 25-OHD3 for the same binding site on the protein. The apparent dissociation constant for 1α, 25-(OH)2D3 and 25-OHD3 was 9.2×10-9 M and 1.5×10-9 M, respectively. The binding capacity was 3.1 pmoles/mg protein for both 1α, 25-(OH)2D3 and 25-OHD3. The order of binding affinity was 25-OHD3>1α, 25-(OH)2D3>>D3=1α-OHD3View full abstractDownload PDF (1426K) -
Yuichi TAKAHASHI, Katsumi URUNO, Shuichi KIMURA1977 Volume 23 Issue 3 Pages 201-209
Published: 1977
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSThe distribution of vitamin E in human serum lipoprotein fraction was investigated by measuring the amount of vitamin E in various protein fractions prepared by ultracentrifugation, gel filtration and electrophoresis. The larger part of the total serum vitamin E (up to 44%) was present in the α1-lipoproteins, while the β-lipoproteins contained smaller concentrations (up to 26%).View full abstractDownload PDF (1203K) -
Masanori YAMANAKA, Tatsuji NOMURA, Masao KAMETAKA1977 Volume 23 Issue 3 Pages 211-220
Published: 1977
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSTo study the role of intestinal microbes in body composition and the effect of Staphylococcus epidermidis (Staph.) on body nitrogen (N) accumulation in host mice, ICR strain male, germ-free (GF) mice, gnotobiotic (GB) mice, obtained from GF mice monocontaminated with Staph. at three weeks of age, and conventional (CV) mice were used. All mice were separated into two groups, one group was killed at five weeks of age (B5) and the other group was killed at eight weeks of age (B8) after being fed an irradiated purified whole-egg protein diet for three weeks. The body weight gains in the three-week period were higher in CV mice than in GF and GB mice. The moisture contents of a carcass per 100 g of body weight were lower in CV mice than in GF and GB mice in both the B5 and B8 groups. The lipid and energy of the carcasses of CV mice in B8 group were higher than in the other mice. Crude protein of the carcasses of CV mice in the B5 group was higher than in the other mice. Accumulation of dry matter, lipid and energy per mouse for three weeks was higher in CV mice than in GF and GB mice. Crude protein accumulation per unit body weight gains in GB mice was higher than that of GF or CV mice. With respect to N and energy retention per unit food intake by the slaughter method, CV mice tended to show high values. The results of N balance by the balance method showed the similar tendencies as the results of the slaughter method. GF mice showed higher values for dry matter, N and energy of gut contents per 100 g of body weight than those of the other mice. Gross energy of crude protein and lipid in carcass showed no differences among the three groups of mice.View full abstractDownload PDF (1142K) -
Masanori YAMANAKA, Tatsuji NOMURA, Masao KAMETAKA1977 Volume 23 Issue 3 Pages 221-226
Published: 1977
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSTo observe the influence of intestinal microbes on energy metabolism, male ICR strain germ-free (GF) mice, gnotobiotic (GB) mice, produced from GF mice monocontaminated with Staphylococcus epidermidis (Staph.) at three weeks of age, and conventional (CV) mice were used. Metabolizable energy, heat production and net energy (gain) per mouse were measured for three weeks when the mice were given 5 Mrad 60Co irradiated purified whole-egg protein diet from 5 to 8 weeks of age. With respect to metabolizable energy and heat production per mouse or per kg of metabolic body size for three weeks, CV mice gave the highest values, followed by GB and GF mice. CV mice showed the highest net energy (gain) followed by GF mice, with GB mice having the lowest value.View full abstractDownload PDF (652K) -
Setsuko YOSHIZAWA, Sachiko MORIUCHI, Norimasa HOSOYA1977 Volume 23 Issue 3 Pages 227-235
Published: 1977
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSThe effect of hydrocortisone and sucrose on the development of chick intestinal disaccharidases was studied using the organ culture system. When intestines of 15- and 17-day-old embryos were cultured in the presence of hydrocortisone, there was significant enhancement of disaccharidases activity compared with the control. However, there was no effect in the 20-day embryonic intestines. On the other hand, the disaccharidase activity of cultured intestines from 20-day-old chick embryos were significantly stimulated by the addition of sucrose. The ob-served increase in disaccharidase activity induced by the administration of hydrocortisone in 17-day-old embryos in vitro was sensitive to actinomy-cin D and cycloheximide. The activity induced by the administration of sucrose in 20-day-old embryos in vitro was sensitive to cycloheximide but insensitive to actinomycin D.View full abstractDownload PDF (968K) -
Fumiko HISAOKA, Keizo SHIRAKI, Sueko SAGAWA1977 Volume 23 Issue 3 Pages 237-247
Published: 1977
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSThe sequestration of erythrocytes in rats was studied using an isologous 51Cr-labeled population of either normal or N-ethyl-maleimide (NEM) treated red cells.
The spleen sequestered the damaged red cells selectively, while the liver compensated and overshot the sequestration for spleen after splenectomy. The sequestering response in liver increased gradually reaching a maximum level around 8 weeks after splenectomy and then declining toward the control level. These compensatory responses in liver were not observed in rats fed a low-protein diet, which indicated that the proliferative response imposed on liver by an extra work after splenectomy was not stimulated in the rats fed a low-protein diet.
Splenectomy prolonged erythrocyte survival and reduced the osmotic fragility of normal red cells, but the compensatory increase in sequestration of damaged red cells in liver did not alter the survival and osmotic fragility of normal red cells of the rat. This fact indicates that the increased sequestration of reticuloendothelial cells in liver is basically reparative, and it is impossible to compensate for the absence of the spleen because of an inability to duplicate certain anatomic features peculiar to the spleen.View full abstractDownload PDF (1286K) -
Sin'itirô KAWAMURA, Kazusi NAGAO, Tasasi KASAI1977 Volume 23 Issue 3 Pages 249-255
Published: 1977
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSAlthough the oligosaccharide contents of soybeans are well documented, the exact values of monosaccharide contents have not been reported. Elaborate methods of preparative paper chromatography together with gas chromatography established the following data for one variety, Kyushu No. 12. The air-dried cotyledon part (admixed with hypocotyls) contained 0.030% glucose and 0.039% fructose. The hull part contained 0.018% galactose, 0.028% glucose, 0.023% fructose, 0.005% arabinose, and 0.002% xylose. Gas chromatograms of trimethyl-silated monosaccharide fractions revealed the existence of minute amounts of sorbitol, arabitol, xylitol, and mannitol in decreasing order (about 0.03% to 0.001% of whole seeds).View full abstractDownload PDF (706K) -
Yoshiki MATSUSHITA, Koichi HASEGAWA, Seima OTOMO, Hirotoshi MORII, Ter ...1977 Volume 23 Issue 3 Pages 257-261
Published: 1977
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSDownload PDF (469K) -
Kiyosi TSUKIDA, Akiko KODAMA, Masayoshi ITO, Mayumi KAWAMOTO, Kumiko T ...1977 Volume 23 Issue 3 Pages 263-264
Published: 1977
Released on J-STAGE: April 28, 2009
JOURNAL FREE ACCESSDownload PDF (216K)
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