In the case of epidemic of poliomyelitis in Ooyubari, Hokkaido, Japan in 1960, the authors had an opportunity to survey the occurrence of poliomyelitis viruses in flies. The results of the survey are reported in this paper. The first case of poliomyelitis patient of this area occurred during the month of April, 1960. During this year, thirty three cases were observed in total population of approximately 16, 000. Since 1955, the fly control operation have continued in this area, and then fly population seemed to be lower than the other areas. In June 29 and 30, just after the peak of occurrence of poliomyelitis cases, the fly collection was carried out. Flies were collected by two methods, netting and baited trapping. The sweeping by net was made over garbage, fish-boxes of fishmonger, privies and the other sites where flies had gathered. Flies collected by net, were poured out into the NaCN killing bottle. For trapping, some cage traps were set over garbage and some others provided with fish baits were hung from the bar in front of the residence. Captured flies were immobilized by chloroform, and killed in the NaCN bottle. After the identification of flies, they were placed in a jar with dry ice, and were sent to the laboratory in Tokyo, where they are kept in a deepfreezer until test. The species and the number of the flies are shown in Table 1. Of 805 individuals of the flies collected, 233 individuals belong to 7 genera and probably more than 7 species were tested for isolation of poliomyelitis viruses. Each sample contains 20 flies or less, and YLE solution was added to it in a rate of 0.5 ml per fly, then it was homogenized in a blender. After the refrigeration, the crude suspension was spun, the sediment discarded. To each 1 ml of supernate, there was added 500 units of penicillin, 500γ of streptomycin and 10γ of aureomycin solution. Tube cultures of HeLa cell were used. To each culture tube, 0.2ml of the inoculum and 0.8ml of YLE solution containing 5% horse serum were added. They were incubated at 37℃ for 7 days. Three passages were carried out every 7 days. Of 14 test samples, 3 proved positive for poliomyelitis virus, which belongs to the type 1. Poliovirus was proved only from samples of Phaenicia sericata. Each 2 tubes out of 7 tubes constituted with one unit sample, in these 3 positive cases, showed cytopathogenic phenomena after 5〜7 days of the inoculation in first generation.
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