The microcirculatory system plays an important role in delivering oxygen and materials to tissues and organs. In this study, we set up a new system with two light sources to measure blood flow velocity, vessel diameter, and pO
2 simultane-ously in organ microcirculation. Pd-meso-tetra-(4-carboxyphenyl)-porphyrin was used as an oxygen-sensitive probe, and was excited with the second harmonic of Nd:YAG pulse laser (wavelength: 532nm) at 1 Hz. The phosphorescence lifetime was obtained from the emission decay curve and pO
2 was calculated from the Stem-Volmer equation. Blood flow in organ microvessels was simultaneously visualized by perfusing red blood cells labeled with fluorescent isothiocyanate. Blood flow velocity and vessel diameter were quantified by processing images with a personal computer. Through measurements on rats
in vivo, the changes in blood flow velocity and pO
2 in organ microvessels were quantified during hemorrhagic shock. The results demonstrate that the system can measure blood flow and oxygen delivery in the microcirculatory system. This sys-tem can be used to analyze pathologic physiology, such as blood flow change in diabetes mellitus.
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