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Volume 14 (1964)
- Issue 3 Pages 97-
- Issue 2 Pages 55-
- Issue 1 Pages 1-
Volume 14, Issue 2
Displaying 1-4 of 4 articles from this issue
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[in Japanese]1964 Volume 14 Issue 2 Pages 55-73
Published: 1964
Released on J-STAGE: March 02, 2010
JOURNAL FREE ACCESSDownload PDF (8445K) -
Masao ONISI, Teiji KATO1964 Volume 14 Issue 2 Pages 74-78
Published: 1964
Released on J-STAGE: March 02, 2010
JOURNAL FREE ACCESSCharacteristic lobate colony of Vibrios were recognized on the Kasai's medium seeded with dental scrapings during three days incubation at 37°C under the anaerobic condition. 15 isolates from 4 specimens, stained negatively by gram staining, were all small sized (1μ±) strongly curved comma shaped organism (B in Figure), moving actively with tufty flaggella, subterminally attached to the concave side of the body (A in Figure). They translocated times like cholera vibrios and times moved rotationary. On solid media containing agar at 3%, they grew into smooth convex colony. They produced neither gas nor putrefactive or disagreeable odor during growth in broth or in glucose broth. Nitrate was reduced. No production of indol and H2S. No coagulation of the litmus milk, but six isolates reduced litmus white. No liquefaction of gelatine. From glucose, they produced acid without gas so far as to decrease the pH of glucose broth until 5.3 finally, but no acid from maltose, lactose, sucrose, glycerol and starch, including two exceptional attack on maltose. Neither greening nor hemolysis on the blood agar plate. Intraperitoneal injection of the cell suspension of this organism to guinea pigs did not cause any significant pathological changes.
The present isolates, thus resembling one species, coincide with neither asaccharolytic Vibrio sputorum which grows only on media enriched with serum and produces H2S, according to Macdonald, nor larger sized Vibrio buccalis which ferments lactose, sucrose, according to Prévot, nor Selenomonas sputigena in respect of the size, the place of attachment of the flagella and activity of carbohydrate fermentation. The most simillar species of the present isolates is small size Vibrio stomatidis which moves rotationary according to Prévot. But this species is virulent to experimental animals and ferments glucose and lactose.
In spite of the above effort, however, it is hardly possible to reach final identification of the present minute vibrio to the known species of the anaerobic commashaped group because of lack of precise knowledges on their flagella. Thus it is strongly proposed by the present authors that flagella of every anaerobic vibrios must be observe under electron microscope which was very useful tool for detection of attached place and number of flagella of the isolates.View full abstractDownload PDF (1761K) -
[in Japanese]1964 Volume 14 Issue 2 Pages 79-86
Published: 1964
Released on J-STAGE: March 02, 2010
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IV. THE UPTAKE OF FLUORINE AND TIN IN OR ON PROTECTED ENAMEL POWDERShoogoro OKADA, Moriyo HINOIDE, Hiromasa AKADA1964 Volume 14 Issue 2 Pages 87-95
Published: 1964
Released on J-STAGE: March 02, 2010
JOURNAL FREE ACCESSIn the recent reseach of topical application of fluoride, Muhler and his coworkers reported that stannous fluoride is more effective than sodium fluoride. In this experiment, the amounts of fluorine or tin in or on protected powdered enamel were determined, which was treated with 2per cent solution of sodium fluoride or 1, 2, 4, 8 or 16 per cent solution of stannous fluoride respectively. The procedure was as follows. Using mechanical shaker, 100mg. of 100-150 mesh enamel powder was treated with sodium fluoride or stannous fluoride solution for 5 minutes at 20°C. respectively, (single application) and in addition, the samples similarly treated four times in repetition were also prepared. For determing fluorine in enamel powder, samples were ashed and steam-distilled, and determined with Zirconium-Alizarin method. Samples protected similarly prepared served also for determination of tin. They were dissolved in 4 N HCl and determined with Cacotherine method. Following conclusions were obtained.
1. Samples treated four times with 2%NaF contained about triple amounts of fluorine as compared with that of singly applied samples.
2. In case of SnF2 application, singly treated samples contained 50-60 μg of fluorine per 100mg, while four times treated one contained fluorine only 10-20 μg over that in single applications mentioned above.
3. The uptake of tin in samples treated with 1% or 2% SnF2 were much more than that treated with 8 % or 16 % SnF2. Such tendency was more marked in case of four times applications.
Considering the uptake of fluorine or tin in enamel together with acid solubility of powdered enamel treated with SnF2, the amounts of uptake of tin appeared to play a dominant role in reducing the acid solubility of enamel, particularly in the earlier decalcifying stage.View full abstractDownload PDF (1383K)
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