From the viewpoint that a biofilm behaves as a biological ecosystem to protect human tissues in general, this study investigated de- and remineralization of enamel with or without the presence of a plaque biofilm formed in vitro using a biological caries model. Experimental microcosm plaque was cultured on bovine enamel blocks in an 'artificial mouth' by supplying a BHI (Difco)-human saliva mixture and 15% sucrose at the rate of 0.1 ml/min on bovine enamel slabs at 37℃ for 5 d resulting in the formation of a caries-like lesion with a lesion depth value 1
d (μm) of 120±9μm (mean±SD). Remineralization tests were done by supplying a mineral solution (1.5 mM CaCl
2, 0.9mMKH
2PO
4, 20 mM Hepes, 2 ppmF as NaF) only or a BHI plus mineral solution mixture on the enamel slabs with or without the experimental plaque biofilm in vitro (n = 6 per treatment). All the samples were microradiographed to measure the 1
d and mineral loss value ΔZ (vol%・μm). After 7-d incubation, the samples treated with BHI-mineral solution mixture under the biofilm showed a significantly lower 1
d value of 51±12 μm and ΔZ value of 1,572±206 vol%・μm (p<0.05) compared with the samples exposed to a mineral solution only under biofilm (1
d = 87±8μm, ΔZ = 1,932±221 vol%・μm) and without biofilm (1
d = 110±14μm, ΔZ = 4,225±1,295 vol%・μm). Thus, plaque biofilm may work as a biological reservoir of mineral and fluoride ions, and thereby contribute to tooth protection by regulating the de- and remineralization process. In conclusion, it is suggested that the biological caries model is applicable and useful as an oral simulator to form a mixed salivary bacteria-based plaque biofilm, and early caries lesions in enamel might be remineralized under biofilm.
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