The BANA test and Perio Check test utilizing dental plaque were developed to examine periodontal conditions. These tests are very easy to use, with practitioners carrying out simple procedures within their own dental practices. However, such tests provide us with very limited information. We are trying to develop a laboratory test at present for judging periodontal conditions. On the other hand, these tests determine proteolytic activity in plaque. It is difficult to use these tests for epidemiological work because sampling sublingual plaque is not so simple here. Salivary components instead of plaque can provide samples for the examination of periodontal conditions. Furthermore, some peptides in saliva demonstrate specific segmentations during proteolysis in saliva. In this study, we examined the mechanisms of segmentation of Histatin 5 by proteolytic activities of human salivary sediments. We found six peptides: DSHAK, HHSHR, HHGYK, HHGY, GY, and RHHGYKRK, during trypsin digestion. After two hours of incubation with human salivary sediments, five peptides, HHGYKR, HHGYK, HHGY, GY, and RHHGYKRK, were found. After four hours of incubation, Histatin 5 had almost disappeared. HHGYKR, HHGYK, and GY were increased, but HHGY and RHHGYKRK were reduced. We examined fragmentation of the N-terminal peptide, DSHAKR, by the salivary sediments. It was found that 90% of the peptide was digested after two hours incubation, but a small amount of DSHAK was newly produced. Histatin 8, which is a C-terminal peptide of Histatin 5, was completely digested within two hours of incubation. It was suggested that assay of the proteolytic activity of salivary sediments using salivary Histatin 5 should be employed for judging periodontal conditions. Further clinical studies are required.
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