Journal of the Japan Society of Blood Transfusion

A CASE OF ANTI-Xg^a INCOMPATIBLE BLOOD TRANSFUSION AND STUDIES OF THE AGGLUTINABILITY OF Xg (a+) CELLS

Fumio Komatsu, Hiromi Satoh, Setsuko Yano, Keiichi Okamura

1982 Volume 28 Issue 2 Pages 121-126
Published: 1982
Released on J-STAGE: March 12, 2010

DOIhttps://doi.org/10.3925/jjtc1958.28.121

JOURNAL FREE ACCESS

Show abstractHide abstract

A patient with maxillary pleomorphic adenoma, aged 57 years, had history of several blood transfusions. He recieved one unit of Xg^a incompatible blood at a emergecy operation in 1980, following fever and red colored urine. Though no significant changes were observed on the levels of GOT, LDH, bilirubin and haptoglobin in his serum, the elevation of anti-Xg^a antibody titer was clearly recognized during about two weeks after the transfusion (from 1:64 to 1:256).
The Xg (a+) cells of male donors (Xg^a: hemizygotes) showed variable values in the titer of agglutinability with anti-Xg^a sera from 1:2 to 1:32, but contrary to these facts, showed no remarkable varieties in the titer with anti-e sera (1:8 or 1:16). These findings suggested that Xg^a antigen had relative large individual variances. The agglutinating reactions of red cells with anti-Xg^a were not observed clearly in some donors specimens stored 10 days. Since the Xg (a+) cells treated by bromelin, papain or ficin usually failed to react with anti-Xg^a sera in the anti-globulin tests, Xg^a antigen might be destroyed by protease commonly used in blood group serology.
It was considerably interesting that the anti-Xg^a titer of this patient elevated markedly after only one unit blood transfusion. Moreover it was suggested that Xg^a-antigenic property may be relatively untable and may degenerate easily during storage, in comparing with Rh-blood group antigens.

View full abstract

Download PDF (701K)
INTERRELATION BETWEEN DEVELOPMENT OF POSTTRANSFUSION HEPATITIS AND LDH₅ ACTIVITY IN TRANSFUSED BLOOD

Jiro Harata, Minoru Nagata, Emi Sasaki, Miyoshi Aoyama, Hidetaka Isaji ...

1982 Volume 28 Issue 2 Pages 127-131
Published: 1982
Released on J-STAGE: March 12, 2010

DOIhttps://doi.org/10.3925/jjtc1958.28.127

JOURNAL FREE ACCESS

Show abstractHide abstract

In a survey of this investigation, the incidence of post-transfusion hepatitis was 23% of patients receiving fresh blood transfusion at open heart surgery, which, as in certain other operations of major surgery, requires massive transfusion of blood. However, LDH₅ assays of pilot plasma specimens from donated fresh blood and avoidance of administering blood showing a high activity of this isoenzyme have led to a marked reduction in the incidence of transfusion-associated hepatitis after open heart surgery.
The activity of LDH₅, an isoenzyme of LDH, was measured after isolation from plasma through a miniture chromatographic DEAE-Sephadex A-50 colum. Operations were performed using only those blood showing low LDH₅ levels of less than 30 Wröblewski units and the patients were followed postoperatively by periodic checkups for a period of three months. As a result, the incidence of posttransfusion hepatitis was found as low as 2.7% or one out of the thirty-seven cases studied. The finding suggests that the measure described provides prevention against the development of posttransfusion hepatitis.

View full abstract

Download PDF (659K)
[title in Japanese]

1982 Volume 28 Issue 2 Pages 160-231
Published: 1982
Released on J-STAGE: March 12, 2010

DOIhttps://doi.org/10.3925/jjtc1958.28.160

JOURNAL FREE ACCESS

Download PDF (9389K)