A 28-year-old housewife suffering from lung tuberculosis, received a transfusion of 1, 200ml of blood without any trouble when a lobectomy of lung was performed on her.
Twenty days after the operation she had to receive a blood transfusion for another operation of thoracoplastik. Soon after setting up the blood transfusion, she complained of a pain along the blood vessel and severe pain in the lower part of the abdomen. The blood transfusion was discontinued when a hundred ml of blood had been transfused. Hemoglobinuria and a rise in NPN were observed
The patient's serum was investigated and the antibodies of Le^a and Le^b were found. These antibodies were postulated to be the ones produced by the former blood transfusion.

View full abstract

In order to find the optmal conditions for the preservation of isolated human erythrocytes, the effects of various factors on ATP formation in isolated human erythrocytes from ACD and heparinized blood were studied. The most important factors in ATP formation in the erythrocytes were the concentration of glucose (0.1M/dl), phosphate (0.01M/dl) and pH(7.4) of the medium containing Ringer's solution.
The most remarkable effect on ATP formation in the long preserved erythrocytes was found in a concurrent addition of Adenine and Inosine as already reported by Nakao et al.

View full abstract

The optimal condition of the medium for the preservation of isolated erythrocytes were investigated by estimating ATP formation rate, hemolytic rate and morphological change of erythrocytes during preservation. It was most important that the medium was isotonic and suitable for ATP formation in the erythrocytes during the preservation. In addition, the better results were obtained with a moderate bufferbase concentration and fully oxygenated erythrocytes.
However it was very difficult to find any inhibitor which acts on rapid glycolysis in the early stage necessary for the long preservation.

View full abstract