A method to detect and measure HB-Ab by means of radioimmunoassay using “Ausria-125” kit (Abbott) is proposed: That is, HB-Ab coated tube and I¹²⁵ labeled HB-Ab are used to test HB-Ab. This method is about 4, 000 times more sensitive than crossimmunoelectrophoretic method.
To test HB-Ab, aliquot amounts of HB-Ag which are about equivalent to HB-Ab coated in each tubes were added in the tubes; after washing the excess of HB-Ag, test solutions containing HB-Ab are added in the HB-Ag bound HB-Ab coated tubes; this is washed and then labeled HB-Ab is added so as to bind all of the remaining HB-Ag; after washing, radioactivity of I¹²⁵ bound in the tubes is counted by scintillation spectrometer.
This method was proved to be negative of the competitive reaction to antigen of antibodies which might be caused by the difference between HB-Ab of the test solution and labeled HB-Ab. Moreover, it was proved convenient to assay both HB-Ag and HB-Ab with same HB-Ab coated tubes and labeled HB-Ab and to compare both results directly. This method is recommended to apply for the routine test as a simple and sensitive tool for screening HB-Ab at high sensitivity.

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The ideal replacement therapy in dental extraction in patients with hemophilia should consist of the minimum but sufficient amount required. We have been pointing out the importance of local hemostatic therapy in the control of hemorrhage from the oral cavity in this disease, along with the replacement therapy. From such a viewpoint, replacement therapy conducted 101 times on 120 teeth during the last 5 years was studied.
The frequency of replacement was 2-2.5 times higher in multirooted teeth than in single rooted teeth. In those with local hemorrhage at the time of extraction versus those without, a larger dosage was given.
Comparison between cases of single extractions and multiple extractions revealed no remarkable difference. Calculation of the amount of replacement in terms of whole blood revealed on the average 556ml per one deciduous tooth and 652ml per one permanent tooth in hemophilia A, while one deciduous tooth required 368ml and one permanent tooth 748ml in hemophilia B.

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