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Volume 47 (2001 - 200･･･
- Issue 6 Pages 829-
- Issue 5 Pages 777-
- Issue 4 Pages 639-
- Issue 3 Pages 363-
- Issue 2 Pages 163-
- Issue 1 Pages 15-
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Volume 47, Issue 3
Displaying 1-14 of 14 articles from this issue
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Hiroyuki Murai2001 Volume 47 Issue 3 Pages 363-368
Published: July 01, 2001
Released on J-STAGE: March 12, 2010
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Takeshi Kawai, Suguru Yamamoto, Yasuyuki Iwatani, Shigeki Miyata2001 Volume 47 Issue 3 Pages 369-377
Published: July 01, 2001
Released on J-STAGE: March 12, 2010
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Chieko Matsumoto, Rieko Shiozawa, Shigeki Mitsunaga, Akiko Ichikawa, R ...2001 Volume 47 Issue 3 Pages 378-383
Published: July 01, 2001
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSA high-throughput nucleic acid testing system was developed for detecting proviral DNA of human T-cell leukemia virus type 1 (HTLV-1), using an automatic nucleic acid extractor and the real-time detection TagMan PCR (TagMan PCR) targeting the pX region of the HTLV-1 genome. Approximately 4μg and 2.5μg of DNA were obtained from 200μl of whole blood and 100μl of frozen blood cells separated from whole blood, respectively. Extraction of nucleic acid from 48 blood samples was completed within 120 minutes. The detection limit of the TagMan PCR was as high as that of the nested PCR. Amplification and detection of HTLV-1 genome in 96 blood samples was completed within 160 minutes. Extraction plus TagMan PCR for viral genome as well as enzyme immunoassay (EIA) and indirect immunofluorescence assay (IF) for HTLV-1-antibodies were performed to test 38 blood samples which were determined to be HTLV-1-antibody positive by the donor screening test using particle agglutination. The results of EIA and IF coincided well with those of the TagMan PCR, indicating that this detection system for HTLV-1 provirus DNA was useful for testing many samples in a short time with high sensitivity and specificity.View full abstractDownload PDF (1980K) -
THE APPLICATION OF PLASMA SAMPLESMika Hosokawa, Shuji Onishi, Misao Abe, Fumiko Okamae, Manabu Yamaoka, ...2001 Volume 47 Issue 3 Pages 384-389
Published: July 01, 2001
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSWe introduced Auto-Vue (Ortho Co.), full automation system of column agglutination technology (CAT) to our range of routine tests in January 1999. Although it is advisable to use plasma samples when automation is a factor, it has been our practice to use serum in conventional tube technique (TT). We compared the titer of various antibodies of EDTA plasma with serum in the Auto-Vue examination and found no difference between them concerning sensitivity of detection. Next, we found that positive samples of self-controls were more numerous in the CAT method than in the TT. No instance of auto-antibody was detected in those samples whose self-controls were positive in CAT and negative in TT. Additionally, it was observed that higher globulin value, lower specific gravity of erythrocytes and higher leukocytes count were frequently associated with false positive results of self-controls in CAT. As a result, plasma samples were useful for detection of antibodies in the CAT method. These results allow for the standardization of transfusion tests, increasing the efficiency of tasks and returning the results of tests more rapidly than before by using the Auto-Vue system.View full abstractDownload PDF (2846K) -
Seiji Mishima, Yoshimori Iwata, Hiroshi Shibata, Junichi Masuda2001 Volume 47 Issue 3 Pages 390-394
Published: July 01, 2001
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSA 68-year-old man was admitted to our hospital with the chief complaint of progressive weakness of the limbs.
Upon neurological examination, he demonstrated facial palsy, flaccid quadriplegia, and demon-strated no response to all deep tendon reflexes stimulation. Laboratory results showed abnormally elevated levels of anti-ganglioside GM1, asialo-GM1, GD1b, and GM3 antibodies. Stool culture for campylobacter species was negative.
A diagnosis of a Guillain-Barré syndrome (GBS) was made and three plasma exchanges were administered. Following the treatment, muscle strength was gradually recovered. Progressive anemia of a mild degree was observed with an increase of unconjugated bilirubin. The direct (DAT) and indirect antiglobulin tests were positive, although the decrease of haptoglobin level was not so pronounced. Testing for Donath-Landsteiner antibody proved negative and cold hemagglutinin test was normal. DAT became negative concomitantly with the recovery from anemia and muscle weakness.
The absorption of purified GM3 resulted in remarkably weak reactivity, indicating the affinity of anti-GM3 antibody with the erythrocyte membrane in this patient. Thus, the presence of anti-GM3 antibody, which has common reactivity with peripheral nerve myelin and erythrocyte membrane, may contribute to GBS becoming complicated with hemolytic anemia.View full abstractDownload PDF (530K) -
Shinichiro Sato, Hiromi Ihara, Shinichi Sakaya, Hideya Hasegawa, Sadam ...2001 Volume 47 Issue 3 Pages 395-402
Published: July 01, 2001
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSDuring the period of November 1997 and October 1999 in Hokkaido, 8 donors were found to be serological screening-negative by agglutination tests yet HBV DNA-positive. Of the 8 samples, 6 were detected as positive by nucleic acid amplification testing (NAT) using 500-pool samples for plasma sources, and 2 were found to be positive by nested PCR using stored samples in the look back study. The HBV DNA concentration in the samples of the 8 donors were widely distributed (range: <100-230, 000copies/ml). Of the 9 patients who were transfused with HBV DNA-positive donor blood, 2 cases were confirmed to be transfusion-transmitted HBV infection. The HBV DNA concentrations of the 2 donors whose blood caused the infection were 230, 000 and 2, 500copies/ml, respectively. In both cases, HBV nucleotide sequences of the patients and donors were identical. Four patients died of primary diseases soon after the transfusion, and no investigation was conducted. Of those patients who were alive and uninfected, 2 were positive for anti-HBs antibodies before the transfusion, and 1 was transfused from the donor with very low concentration of HBV DNA (<100copies/ml), although the patient was anti-HBs antibody negative before the transfusion. These results suggest that the risk of HBV infection in transfusion in Japan may be higher than that has been anticipated.View full abstractDownload PDF (3303K) -
Tetsu Yamamoto, Yutaka Tomoda, Kuniko Fukui2001 Volume 47 Issue 3 Pages 403-404
Published: July 01, 2001
Released on J-STAGE: March 12, 2010
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2001 Volume 47 Issue 3 Pages 423-461
Published: July 01, 2001
Released on J-STAGE: March 12, 2010
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2001 Volume 47 Issue 3 Pages 462-502
Published: July 01, 2001
Released on J-STAGE: March 12, 2010
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2001 Volume 47 Issue 3 Pages 503-542
Published: July 01, 2001
Released on J-STAGE: March 12, 2010
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2001 Volume 47 Issue 3 Pages 543-582
Published: July 01, 2001
Released on J-STAGE: March 12, 2010
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2001 Volume 47 Issue 3 Pages 583-617
Published: July 01, 2001
Released on J-STAGE: March 12, 2010
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2001 Volume 47 Issue 3 Pages 618-621
Published: July 01, 2001
Released on J-STAGE: March 12, 2010
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2001 Volume 47 Issue 3 Pages 622-623
Published: July 01, 2001
Released on J-STAGE: March 12, 2010
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