All issues
Volume 45 (1999)
- Issue 6 Pages 661-
- Issue 5 Pages 599-
- Issue 4 Pages 425-
- Issue 3 Pages 339-
- Issue 2 Pages 119-
- Issue 1 Pages 1-
Predecessor
Volume 45, Issue 5
Displaying 1-8 of 8 articles from this issue
- |<
- <
- 1
- >
- >|
-
Keiya Ozawa1999 Volume 45 Issue 5 Pages 599-604
Published: October 01, 1999
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSDownload PDF (874K) -
Yoshiko Yamada, Hideki Abe, Kenji Ikebuchi, Sadayoshi Sekiguchi1999 Volume 45 Issue 5 Pages 605-610
Published: October 01, 1999
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSTreatment with psoralens and ultraviolet-A (UVA) irradiation have been found to be effective for virus sterilization of platelet concentrates (PCs). We report here a virus inactivation method using a combination of psoralen derivative 4′-aminomethyl-4, 5′, 8-trimethylpsoralen (AMT) and UVA irradiation (AMT/UVA). Further, we also investigated the effect of rutin, a radical scavenger, on the inactivation of vesicular stomatitis virus (VSV) as a model virus administered in PCs and platelet functions were investigated.
Spiked VSV (about 5log10) in PCs was inactivated by a combination of AMT (50μg/ml) and 5.2J/cm2 UVA irradiation in the absence of rutin. To obtain equivalent levels of VSV kill in the presence of 0.35mM rutin, treatment with 13.0J/cm2 of UVA irradiation with AMT was performed.
When PCs were treated under each condition in which 5log10 VSV was inactivated by AMT/UVA with or without rutin, platelet aggregation function was maintained for more than 80% of untreated platelets. These findings indicate that the presence of rutin during AMT/UVA treatment conferred no beneficial effect. In addition, overnight storage of PCs with AMT induced 40% loss of platelet aggregation in response to 10μM ADP. The findings suggest that UVA irradiation is required immediately after the addition of AMT.View full abstractDownload PDF (2105K) -
Shingo Yano, Osamu Asai, Akinori Kato, Hiroshi Watanabe, Mituji Katori ...1999 Volume 45 Issue 5 Pages 611-616
Published: October 01, 1999
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSTo investigate the feasibility of CD34-positive selection, we separated CD34+ cells from KGla cells, fresh bone marrow samples (obtained from 2 ALL patients, 1 NHL, 1 breast cancer and 4 healthy volunteers) and frozen samples (3 ALL) by the avidin-biotin immunoadsorption using a CEPRATE LC KITS® (CellPro Inc.). We then detected minimal residual disease (MRD) from a separated Ph-positive ALL fresh sample by the PCR method. The yield of selected CD34+ cells were 78.9% (KGla cells), 45.5% (fresh bone marrow sample obtained from ALL, NHL and breast cancer), 9.3% (healthy volunteers) and 3.9% (frozen sample obtained from ALL). A sample of Ph ALL was able to eliminate MRD from the CD34+ fraction. We conclude that CD34-positive selection by the avidinbiotin immunoadsorption is useful in fresh samples but not from frozen samples and we demonstrated that this procedure may deplete tumor cells in a patient with ALL.View full abstractDownload PDF (2474K) -
Yoshiyuki Kurata, Shoichi Inaba1999 Volume 45 Issue 5 Pages 617-622
Published: October 01, 1999
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSWe surveyed medical curricula devoted to transfusion medicine for medical students in the year of 1997. All medical universities in Japan were included in this questionnaire survey (80 universities). Answers were received from all the universities. Only 29 of 80 universities had specific curricula devoted to transfusion medicine. Lecture time was 1.9±3.3 hours in the course of 6 years. Transfusion medicine was taught in a variety of courses, such as legal medicine, internal medicine, etc. Total lecture time regarding transfusion medicine was 7.6±6.5 hours. Forty-one universities had training time for teaching how to test blood typing and compatibility testing, and time to discuss the use of blood products, but total training time was only 3.7±6.0 hours. Legal medicine and laboratory medicine also included training time regarding transfusion medicine, with total training and discussion time regarding transfusion medicine of 8.3±6.1 hours. We believe it is necessary for all medical universities to have specific curricula devoted to transfusion medicine.View full abstractDownload PDF (5495K) -
Evaluation of automatic extraction system GT-12 in HCV RNA testingKoichi Noguchi, Takasi Murozuka, Akemi Wakisaka, Syuzo Matumoto1999 Volume 45 Issue 5 Pages 623-628
Published: October 01, 1999
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSSince NAT is a highly sensitive technique for detecting low amounts of viral genomes, it is applied to the screening of source plasma to reduce the risk of viral infection through plasma-derived blood products.
For this purpose we have used an NAT test for HBV, HCV and HIV by PCR in pooled plasma from of 500 individuals since November, 1997.
Because nucleic acid extraction is the most time-consuming and laborious job in this NAT test, an automated method was sought to reduce the operation time and human error. This would allow processing of more samples than the currently used extraction.
Recently, Roche Diagnostic K. K. has developed an automated extraction system named GT-12 and an extraction reagent for HCV RNA. In this study we compared GT-12 with the manual technique. The results show GT-12 extraction caused no contamination and had good reproducibility without any inter-day variation. Although GT-12 is less sensitive than the manual extraction method, it is sensitive enough to detect 100IU/ml as required by the CPMP guideline (CPMP/BWP/390/97). Operation time of extraction by GT-12 was about 2/3 of that by manual extraction. Introduction of GT-12 may contribute to the increased efficiency of the NAT test.View full abstractDownload PDF (680K) -
Kikuyo Taniguchi, Masao Kobayashi, Misae Tanihiro, Asako Hiraoka, Kosu ...1999 Volume 45 Issue 5 Pages 629-631
Published: October 01, 1999
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSDownload PDF (253K) -
Yoshiharu Kaneko, Akie Iizumi, Kumiko Sakairi, Takako Nakamura, Masayo ...1999 Volume 45 Issue 5 Pages 632-635
Published: October 01, 1999
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSLan is a frequently occurring red blood cell antigen observed in over 99.9% of the population. We describe herein a 42-year-old Japanese man who developed anti-Lan antibodies after transfusion of three units of packed red blood cells. His red cells and those from one of his four brothers were found to be Lan-negative, whereas those from the rest of his family, including his parents and his two children, were Lan-positive. His parents were first cousins. To evaluate the frequency of Lan antigen in Japanese living in Ibaraki Prefecture, we screened red blood cells from 4100 unrelated individuals by antiglobulin testing of the patient's serum. No other persons lacking Lan antigen were found.View full abstractDownload PDF (470K) -
[in Japanese]1999 Volume 45 Issue 5 Pages 658-659
Published: October 01, 1999
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSDownload PDF (240K)
- |<
- <
- 1
- >
- >|