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Volume 31 (1985)
- Issue 6 Pages 535-
- Issue 5 Pages 369-
- Issue 4 Pages 285-
- Issue 3 Pages 205-
- Issue 2 Pages 103-
- Issue 1 Pages 1-
Predecessor
Volume 31, Issue 6
Displaying 1-8 of 8 articles from this issue
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Toshio Mazda, Kenichi Nakata, Kanji Morimoto, Masaru Shimizu1985 Volume 31 Issue 6 Pages 535-542
Published: 1985
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSReacent studies show that monitoring guanase activity in transfusion blood may be useful in preventing post-transfusion non-A non-B hepatitis. We measured serum guanase activities in samples from blood donors at the Japanese Red Cross Tokyo Metropolitan Blood Center by three different methods: a 1 channel automatic (1 ch) method, a 2 channel automatic (2 ch) method and a manual method. SGPT and SGOT activities were also determined on the same samples by an automatic kind & King assay, using an Olympus ACA 6008N automatic analyzer. Both donors with high guanase activity and recipients transfused blood with high guanase activity were followed.
In low guanase activity (normal) donors, the correlation among the three guanase methods was very poor. The correlation between guanase activity and SGPT or SGOT in all blood donors was excellent. The high ganase activity donors often showed low guanase activity after 6 months. Recipients with high guanase activity blood did not always contract post-transfusion hepatitis.
The data suggest that improvements in guanase activity assays must be made. A screening test of guanase activity in transfusion blood is not deemed necessary at this time.View full abstractDownload PDF (878K) -
Hitoshi Yoshida, Akira Fukui, Osamu Fukutomi, Masuhiko Takaori1985 Volume 31 Issue 6 Pages 543-548
Published: 1985
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSWe surveyed all of blood units prepared and transfused for elective surgery, particularly 13 kinds of major operation, in our clinic for three annual terms, i. e. 1976, 1979 and 1982. Averaged amount prepared for transfusion was 6.9±3.2 units (one unit=200ml) for each operation and 47±19% of those was returned back to blood supply centre in our hospital from either our operation theater or ICU during the 3 years. Un-utilized units in the prepared blood were reduced in all of gynecological operations in 1982 compared with those in 1979, particularly from 60% to 3% with simple hysterectomy for myoma uteri. On the other hand, the un-utilized rate increased with mastectomy, rectum amputation and laminectomy, namely 22→41, 39→50 and 22→41% respectively. Either hemodilutional autotransfusion alone or its combination with homologous blood transfusion was used in all of operations, un-utilized rates of which were less than 50%, except mastectomy. On the other hand, un-utilized rate exceeded over 50% with gasterectomy, rectum amputation, laminectomy, open-heart or non-open-heart surgery and removal of brain tumor. Average of C/T ratio (units tested for cross matching/units transfused) remained mostly unchanged, as 2.07 in 1976, 2.11 in 1979 and 2.10 in 1982.View full abstractDownload PDF (818K) -
Tetsuo Shimizu, Yukiko Sekido, Kiyomi Morizawa, Yuko Ishikawa, Satomi ...1985 Volume 31 Issue 6 Pages 549-555
Published: 1985
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSPlatelets derived from platelet pheresis were frozen with 5% dimethylsulfoxide (Me2SO) using a programable freezer at a rate of 1°C/min until -80°C, and then stored in a liquid phase of liquid nitrogen. The function and cellular components of thawed and reconstituted platelets were compared using gel-filtered platelets with those of 5% glycerol. The overall results obtained here indicated that platelets cryopreserved with Me2SO retained higher function and more cellular components than those of platelets cryopreserved with 5% glycerol.
Thawed platelets with or without 5% Me2SO were stored at 22°C for 6 hours to investigate effects of presence of Me2SO in platelet suspension. Removal of Me2SO from thawed platelets did not alter significantly the functional and metabolic changes in platelets for up to 6 hours at 22°C.View full abstractDownload PDF (795K) -
Nobuo Nagao, Hideto Ogawa, Atsuko Taniue, Satoshi Ohtani, Kazue Konish ...1985 Volume 31 Issue 6 Pages 556-560
Published: 1985
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSThe proposita was a healthy 51-year-old female donor who was brought to our attention when her serum reacted with all the cells tested except two Ko samples by saline, bromelin, albumin and anti-globulin methods.
The proposita's cells failed to react with anti-K, anti-k, anti-Kpa, anti-Kpb, anti-Kpc, anti-Jsa, anti-Jsb and anti-Ku as expected. These negative reactions were reconfirmed by absorption-elution tests with appropriate sera. These findings indicated that the proposita was Ko with anti-Ku in her serum. Furthermore, she was found to be SS besides Ko, which is exceedingly rare in Japanese.
A family study disclosed that the Ko phenotype occurred in two successive generations.
Our experiments showed that the three samples of anti-Ku including the proposita's were absorbed by cells from the platelets layer, but the other antibodies of the Kell system such as anti-k, anti-Kpb, anti-Kpc and anti-Jsb were not.View full abstractDownload PDF (509K) -
1985 Volume 31 Issue 6 Pages 561-611
Published: 1985
Released on J-STAGE: March 12, 2010
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1985 Volume 31 Issue 6 Pages 611-637
Published: 1985
Released on J-STAGE: March 12, 2010
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1985 Volume 31 Issue 6 Pages 637-668
Published: 1985
Released on J-STAGE: March 12, 2010
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1985 Volume 31 Issue 6 Pages 669-673
Published: 1985
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSDownload PDF (704K)
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