Recently, we obtained a mixture of antibodies, detected by cross-matching test, in a serum of a patient suffering from spinal caries.
Direct Coombs test was strongly positive, and the antibody in her serum weakly agglutinated her own erythrocytes and many other normal cells, by treatment with bromelin, at 37°C. It may be regarded as a warm autoantibody of acquired haemolytic anaemia.
Other antibodies are the following:
1. Anti- M. Active in saline and weakly in AB-serum at 20°C and 4°C. It may be probably a naturally occurring antibody.
2. Anti- E. Active in AB-serum, and in saline with bromelin-treated cells at 37°C and 20°C, and by the indirect Coombs technique. It seems probably to result from the immunization due to previous transfusions.
These antibodies were also detected and confirmed through the kindness of Dr. P. Levine of the Ortho Research Foundation.

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A major problem concerned with the performance of cardiac operations during extracorporeal circulation has been the procurement and preservation of the large amounts of blood necessary for this type of operation. Because of the toxic effects of citrated blood, heparin has, until recently, been the only satisfactory anticoagulant available. On account of biochemical and hematological alterations the fresh heparinized blood usually has beenn used in the intracardiac operations using artificial pump-oxygenator. We have felt strongly the necessity of the preservation of heparinzed blood as increasing of cardiac operations. It was the purpose of the present study to compare the safety of fresh and stored heparinized blood for use in extracorporeal circulation analyzing the chemical changes. The particulars about my researches are as follows:
1) The preservation of heparinized blood for long period is difficult because of coagulation of blood in short period.
2) I can recommend to use the heparinized blood within 24 hours. But better to use it as soon as possible.
3) Six mg heparin should be added in 200ml blood. Excessive usage of heparin is not desirable.
4) Additional usage of heparin in the course of preservation of blood had no effect on the prevention against fibrinization and coagulation.
5) Amino acid and adenine as the origin of energy inhibit the production of lactic acid which occurs during the preservation of heparnized blood.
6) Life of R. B. C. of heparinized blood is shorter than that of A. C. D. blood (Acid-citrate-dextrose blood). Addition of amino acid and adenine had no effect on the prolongation of the life of R. B. C.

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After two and half years experiments we have reached the conclusion that the electrodermatography is an effective screening of donors to prevent the posttransfusion hepatitis.

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In the postoperative period of patients with gastrointestinal diseases, due to the disturbance of oral diet intake or discontinuence of intake as well as catabolic response of surgery, body protein loss increases and often results in unpleasant complication. The author studied about 88 patients who were operated upon for their G. I. tract diseases at Keio University Hospital, of protein metabolism in their early postoperative period from the changes of serum protein and nitrogen balance. Also effects of administration of amino acids preparation, glucose and anabolic steroid were observed. Furthermore, the effects of administration of amino acids preparation and anabolic steroid were studied upon the influencing factors of the postoperative protein metabolism such as nutritional status, age, sex, size of surgery, duration of operation, disturbed liver function and presence of cancer.

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