Concerning the 213 courses of replacement therapy (202 for hemophilia A and 11 for hemophilia B) conducted on hemorrhage from the oral mucosa in a total of 244 cases of hemophilia (209 hemophilia A and 35 hemophilia B) during the past 5 years, omitting 1 case of dental extraction already reported, a study was conducted as to whether or not the frequency and amount of replacement therapy for hemostasis was adequate.
The frequency of replacement therapy was less than one on an average for gingiva, lips, cheek and nose requiring relatively easy local hemostatic measures. For hemorrhage from the tongue, palate and tonsils requiring difficult local hemostatic measures, more than 2 replacements were required. When the amount of various replacementalagents infused was calculated as whole blood volume, hemorrhage from the gingiva required 259ml in hemophilia A and 280ml in hemophilia B per one hemorrhage episode, hemorrhage from the dental pulp required 45ml in hemophilia A, and 20ml in hemophilia B, hemorrhage from the tongue required 913ml in hemophilia A, hemorrhage from the lip required 267ml in hemophilia A, hemorrhage from the nose required 360ml in hemophilia A and hemorrhage from other sites (cheek, tonsils) required 1049ml in hemophilia A. As to the age difference, the amount of blood required per one bleeding episode was compared in patients below and above 5 years of age. In the young with difficult local hemostatic measures, large amounts of blood, 2-5 times that on other occasions were used.
Local hemostatic measures thus appear to be important in the control of hemorrhage from the oral cavity, especially in infants and children in whom local treatment is quite difficult.

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Various factors affecting Au antigen sensitization of glutaraldehyde-treated erythrocytes by tannic acid method were explored extensively.
The main results were as follows.
An increased ratio of morphologically abnormal cells in fixed cells was obtained with increasing concentrations of glutaraldehyde with which fresh cells were treated. When Au antigen was coated to the cell preparations containing high ratio of abnormal cells, the sensitized cells tended to be agglutinated non-specifically and form indistinct settling patterns. The capacities for sensitization of fixed cells did not vary appreciably with varying times of exposure to glutaraldehyde or varying concentrations of the reagent. But fixed cells was shown to have lower capacities for sensitization with increasing times for storage at 4°C.
The cells treated with increasing tannic acid concentrations or increasing antigen concentrations yielded higher titers against standard antisera, but the cells treated with high concentrations of tannic acid as well as Au antigen tended to be agglutinated non-specifically and form indistinct settling patterns. Cells incubated 5min. or longer, gave essentially similar titers with standard antisera, but the uptake of Au antigen by cells was increased gradually untill 20min. The results of sensitization with Au antigen were appreciably varied with different lots of Au antigen preparations as well as different lots of cells.

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