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[in Japanese]
1986 Volume 15 Issue 3 Pages
1549
Published: June 15, 1986
Released on J-STAGE: October 07, 2011
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H. IWAMOTO, Y. NAKAMURA, T. SHIBAMOTO, S. NAKAGAWA
1986 Volume 15 Issue 3 Pages
1550-1553
Published: June 15, 1986
Released on J-STAGE: October 07, 2011
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In order to reduce loss of albumin in double filtration plasmapheresis (DFPP), we evaluated a new system of DFPP which incorporates an automatic backwash system of the second filter. The effects of back-wash system of DFPP were compared with those of conventional partial discard system of DFPP. Each system of DFPP was performed six times in a case of homozygous familial hypercholesterolemia. There were no significant differences between two groups in pre- and post-treatment serum total cholesterol levels and the amount of total cholesterol removed per session. Pre-treatment levels of serum albumin were not significantly different between the two groups, but post-treatment serum albumin levels were significantly higher and the amount of albumin removed was significantly less in back-wash system than partial discard system of DFPP. It was concluded that the new back-wash system of DFPP was more effective than partial discard system of DFPP.
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A. SAITO, H. OGAWA, T. TOYOMI, S. KENJI, K. SHINICHIRO, Y. SHIRO
1986 Volume 15 Issue 3 Pages
1554-1557
Published: June 15, 1986
Released on J-STAGE: October 07, 2011
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SAC-plasmapheresis were given once per week to 4 chronic rheumatoid arthritis patients. Then double filtration (DF) plasmapheresis and cryofiltration (CF) were performed on the same patients with the same frequency. The results of treatment and the rate of macromolecule removal were compared. The reduction rate of plasma albumin (Alb) in SAC, DF and CF methods was 15.9±4.3, 11.3±2.8 and 16.1±3.9%, respectively; and the plasma IgG reduction rate was 41.2±6.6, 20.2±10.8, and 11.7±1.9%, respectively. The plasma IgA reduction rate was 37.9±7.0, 19.4±5.7, and 14.7±3.3%, respectively. IgG and IgA reduction rates of SAC thus showed the highest values of all. The plasma IgM reduction rate was 34.9±7.0, 45.6±2.3, and 20.2±5.8%, respectively. Hence the DF method achieved higher levels than SAC approach. After receiving the SAC, DF and CF procedures for one month, the grasping power of the 4 patients tended to be greater than the pre-treatment value. However, only SAC obtained a significant increase in grasping power.
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Nobuhiko KOGA, Hirokuni SASAKI, Tadashi NISHIMURA
1986 Volume 15 Issue 3 Pages
1558-1565
Published: June 15, 1986
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In clinical Double Filtration Plasmapheresis (DFPP) (Recycle Method) the processing of a plasma volume of more than 3000mls requires a change, or rinse of the second filter, and rinsing is preferred for economic reasons. In processing a regular quantity of 4000mls each session, the second filter been reverse rinsed at either half-volume (2000mls) processed or at reaching a transmembrane pressure gradient of 400mmHg across the second filter. Either of these could be used to indicate when to rinse the filter. We evaluated the effects of each method on removal rates of the plasma components, and found that when rinsing at half-volume, the removal rate of albumin was 13.7% and of HDL-cholesterol was 20.0%, and when rinsing at reaching 400mmHg transmembrane pressure the removal rates were 20.5% for albumin and 44.1% for HDL-cholesterol. We conclude and recommend therefore that the second filter be reverse rinsed after processing a half volume of 2000mls.
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T MIYAHARA, A SUEOKA, T SUZUKI, Y UEDA, N YAMADA, Y IKEDA, Y YAGIRI
1986 Volume 15 Issue 3 Pages
1566-1569
Published: June 15, 1986
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Based on the clinical data obtained by applying a new plasma filtration system utilizing EVAL plasma fitter in combination with a centrifugal plasma separator, filtration characteristics of EVAL filters were discussed. Reduction rate of macromolecules well correlated to sieving coefficients of each filter indicating the sieving of filters relate directly to selective removal of macromolecules. Sieving and filtrate volume changed depending on the plasma composition varied with diseases. Although about 15% of platelet in whole blood was found in plasma separated by the centrifuge, it had little effect on filtration properties of EVAL filter. Sieving did not show any difference whether blood was anticoagulated with ACD-A or heparin. Effective plasma filtration was performed by EVAL filters in combination with centrifugal plasma separation.
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T. YANAI, M. IKEGAME, K. HASEGAWA, T. FUJISHIRO, S. MURAI, K. KOJIMA, ...
1986 Volume 15 Issue 3 Pages
1570-1573
Published: June 15, 1986
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In the clinical study the filtration efficiency of the prediluted plasma separation was compared with the conventional method of single and double membrane plasma exchange. From these comparative studies prediluted plasma separation have high efficiency in filtration flux, selective removal of pathogenetic proteins and sparing plasma albumin. The results from the in vitro plasma separation using kuraray t s minifilters of 2A and 4A approved the availability of prediluted plasma separation in single and double membrane plasma separation.
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[in Japanese]
1986 Volume 15 Issue 3 Pages
1574
Published: June 15, 1986
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M. MINESHINA, T. AGISHI, I. KANEKO, Y. HASUO, K. ERA, K. OTA, K. SAKAI
1986 Volume 15 Issue 3 Pages
1575-1578
Published: June 15, 1986
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Sieving coefficient(SC) have been used for evaluating the performance of ultrafilters. Although several SCs derived from different definitions have been already presented, all these are not real but apparent parameters. In this paper, the differences in performance using these parameters were clarified by an analysis of clinical data and in-vitro experiments. Differences of the performance evaluation between several SCs increased as filtration fraction increased. Real SC (SCreal) was obtained as the intercept of the ordinate by a realtion between the reciprocal of inlet flow rate and observed SC.
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K. TAKAYAMA, N. KANAMORI, M. WAKASA, H. KOJIMA, T. AKIZAWA, T. KITAOKA ...
1986 Volume 15 Issue 3 Pages
1579-1582
Published: June 15, 1986
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Several kinds of filters have been utilized to remove leucocytes and microaggregates through blood transfusion. In order to apply these filters to leucocytapheresis (LP), we examined the adhesive and activating capacity for blood cell components and complement levels respectively. Among three kinds of commercially available filters, Sepacell R 500 (S) showed the highest removal capacity for leucocyte and the absence of complement activation. In ex vivo LP with dogs, S showed sufficient adhesive capacity for leucocyte and coagulation during LP could be completely prevented by the use of ACD or nafamostat mesilate as anticoagulants. These results indicate that LP with S can be applicable clinically, but further improvements in the adhesive selectivity may be desirable.
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S. KUBOTA, S. HAMABE, H. KOMORIYAMA, T. KANAZAWA, S. OHWADA, Y. MARUYA ...
1986 Volume 15 Issue 3 Pages
1583-1586
Published: June 15, 1986
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In order to remove selectively immunoglobulins and complexes, plasma was dialysed and then added with acetic acid, ascorbic acid and glutamic acid. The plasma was dialysed by deionized water with 500ml/min. Dialysed plasma was precipitated, but removal rates in plasma components except IgM were low. When Ph of dialysed plasma was adjusted to 5.0-6.8 with acids, immunoglobulins and complements were precipitated with higher selectivity than albumin. The recovery rates of albumin were 89±2.5% in PH 6.7 and 88±2.1% in PH 5.7. On the other hand, the recovery rates of IgM were 39±5.9% in Ph 6.7 and 38±6.5% In PH 5.7. C
3 and Cps recovery rates in PH 5.7 were 24±2.7% and 9.5±1.0%, respectivly. However, the recovery rats of IgG in PH 5.7 was slightly high.
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A SUEOKA, T MIYAHARA, T SUZUKI, Y UEDA, T HORIUCHI, M USAMI, P MALCHES ...
1986 Volume 15 Issue 3 Pages
1587-1590
Published: June 15, 1986
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Effect of temperature on filtration characteristics of EVAL filter was studied in vitro and in clinical test. Larger volume was processed as the temperature was increased up to 42°C. 1.3-1.6 times as large volume was processed in physiological temperature range (37-42°C) as that processed at ambient temperature (28-32°C). Generally, sieving coefficients of macromolecules increased with increasing temperature. Selective separation of macromolecules such as HDL and LDL was achieved in plasma filtration at 37-42°C. Increase in filtration volume with increasing temperature up to 42°C would be mainly related to lowering plasma viscosity with increasing temperature. It was found precipitates were formed in plasma at higher temperature, particularly avove 45°C, and then processed volume decreased compared with that at 37-42°C. Suggestive filtration temperature would be 37-42°C thinking over filtration efficiency and thermostability of macromolecules.
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T. YOSIOKA, T. OKA, K. HANASWA, Y. ENDO, K. MATSUDA, T. TANI, K. FUKUI ...
1986 Volume 15 Issue 3 Pages
1591-1595
Published: June 15, 1986
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Pre-clinical studies has been required for evaluating the biocompatibility and the safty of newly developed material. When extracorporeal circulations wereperformed on dogs with clinical circuits and material, the temperature of them decreased gradually. But few paper mentioned the importance of well-conditibned temperature of experimental animal body. In our studies, shortened circuits kept body temperature well. Body temperature affected blood cell kinetics and complement titer, which were one of the best parameter of biocompatibility. Preservation of body temperature is necessary to evaluate the biocompatibility, the effect and the safety of blood perfused artificial organs in experimental animals.
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T. MURAJI, E. OKAMOTO, S. HOQUE, A. TOYOSAKA, J. FUJIMOTO, H. IFUKU, Y ...
1986 Volume 15 Issue 3 Pages
1596-1598
Published: June 15, 1986
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Animal experiments were undertaken to determine the feasibility of plasma exchange in neonates and infants. Five mongrel puppies weighing 2-5kg underwent plasma exchange using a venovenous extracorporeal circuit of 80ml of priming volume, in which a membrane plasma separator (surface area: 0.3 or 0.15m
2) was inserted. When the 18G or 16G catheter was used, 25 or 40ml/min. of blood flow rate was respectively obtained without increasing line pressure over 100mmHg. A transmembrane pressure was around 0mmHg when plasma flow rate was 1/3-1/4 of blood flow rate. Within 60 minutes, 100ml/kg of fresh frozen plasma was replaced in a hemodynamically stable condition. However, hematocrit decreased by 5-40% after the exchange because of priming the circuit with plasma. Based on these data, the plasma exchange for neonates and infants seems to be feasible.
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[in Japanese]
1986 Volume 15 Issue 3 Pages
1599
Published: June 15, 1986
Released on J-STAGE: October 07, 2011
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T. TANI, A. FUKUTANI, T. YOSHIOKA, T. OKA, K. HANASAWA, Y. ENDO, N. TE ...
1986 Volume 15 Issue 3 Pages
1600-1604
Published: June 15, 1986
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Immunosuppressed plasma of cancer-bearing patients were filtrated by EVAL-C (KF-101) in the experimental closed circuit. Rejection rate of alubumin by EVAL-C is 84%. Immunosuppression was presented by influence of mitogen (PHA and ConA)-induced blastogenesis of normal lymphocyte. Filtrate mostly showed improved immunosuppression compared with that of original. But some case showed the strong suppression in the filtrate. The suppression of others improved markedly both in the filtrate and residual. Heat-inactivation of plasma was performed heating at 56°C for 30 minutes. This preparation improved the immunosuppressed plasma to the normal level. And it made the immunosuppression of plasma better on residual than original plasma. According to these facts, filtrate by protein permiable hemofilter is to be discarded, and heat-inactivation on residual would be a good immunomodulating therapy for the far advanced cancer patients which saves much amount of fresh frozen plasma.
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Y. NISHOKA, K. SAKAGAMI, M. MIYAZAKI, J. MATSUOKA, T. MATSUNAGA, S. SH ...
1986 Volume 15 Issue 3 Pages
1605-1608
Published: June 15, 1986
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We studied whether plasma exchange (PEx) possessed the augmentative efficacy to cancer immunochemotherapy. We supposed the existence of the factors in cancer-bearing sera that supressed the anti-tumor effect of OH-1 against tumor metastasis after removing these factors in the sera with PEx, immunochemotherapy was performed PEx possessed the augmentatiue effect any case when combined with immunotherapy with OH-1, chemotherapy with Cyclophosphamide, and immunochemotherapy simultaneouly, and suppressed the tumor growth significantly. Consequently, the role of PEx among various cancer therapies is clarified, and further selective removing of various immunosuppressive factors become demanded in clinical cases.
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T. MATSUNAGN, K. SAKAGAMI, Y. NISHIOKA, M. MIYAZAKI, J. MATSUOKA, S. S ...
1986 Volume 15 Issue 3 Pages
1609-1612
Published: June 15, 1986
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Protein A, a cell wall component of Staphylococcous aure us which has the ability to bind immunogloburins and immune complexes has recently been demonstrated as an immunoadsorbent in the treatment of animal and human cancer. In this study, ex vivo plasma adsorption over native or recombinant, protein A, which is free of Staphylococcal toxin, were performed to cancer bearing rat. Plasma adsorption over native protein A remarkably inhibited tumor growth, compared with control rats. The treatment using recombinant protein A showed the same inhibition of tumor growth as that .using native protein A. Direct inj ction of native or recombinant protein A into the cancer bearing rat did not showed any significant inhibition of tumor growth. These results indicated that the tumoricidal activity of protein A did not result from Staphylococcal toxin.
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Y. ITO, H. HIRASAWA, H. KOBAYASHI, K. SOEDA, N. MUROTANI, I. OHSHIMA, ...
1986 Volume 15 Issue 3 Pages
1613-1615
Published: June 15, 1986
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The present experimental study was undertaken to establish the massive anticancer agent therapy in combination with hemoadsorption aiming the increase in anticancer effect and the simultaneous decrease in adverse effect. The study revealed that CDDP was effectively removed from the blood by the hemoadsorption and that the simaltaneous application of hemoadsorption could decrease the renal damage by the agent despite of high concentration of the agent in the esophageal tissue. These results sugciest that this therapy could be applied in the clinical settings.
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evaluation of the adsorbability of various adsorbents for low molecular weight immunosuppressive factors
S. SHIOZAKI, K. SAKAGAMI, M. MIYAZAKI, J. MATSUOKA, V. NISHIOKA, T. MA ...
1986 Volume 15 Issue 3 Pages
1616-1620
Published: June 15, 1986
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It is well known that immunosuppressive factors (ISFs) in the sera of cancer patient consist of high and low molecular weight (m. w.) fractions. We have previously reported that DFPP could effectively eliminated high m. w. ISFs, but could not remove low m. w. ISFs In the present study, we examined the ability of various adsorbents to adsorb the low m w. ISFs in order to determine the most suitable adsorbent combined with DFPP. In conclusion, NH
2- coated glassbeads adsorbent with pore size of 100Å showed the maximam removal rate of IAP, α
1-acidglycoprotein, and PHA inhibitory factors. These results indicated that NH
2-coatedglassbeads adsorbent with pore size of 100Å should be the most suitable adsorbent combined with DFPP at clinical application.
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H. KANAKA, K. AKIMARU, K. HARA, S. KANAUCHI, T. SHOJI, K. SHIMIZU, O. ...
1986 Volume 15 Issue 3 Pages
1621-1624
Published: June 15, 1986
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We utilized ‘Nacl-glycine.precipitation method’ to remove immunosuppressive factors from cancer patients by use of modification of plasmapheresis system. The plasma was separated by a diacetate cellulose membrane and collected with 750 grams of Hadl and 450 grams of glycine in a plastic bag, and the bag was stored in 4°C for three nights. The precipitation was removed by a filter membrane, and satulated Nacl and glycine was removed by H. D. In the first treatment, 5% Alubumin solution was used as replacement fluid and from second treatment, manipulated own plasma was used as replacement fluid. In this method, removal of globulin fraction, mainly IgG, was significant however removal of Alubimin was minimum. Using of inhibition of normal human lymphocytes blastgenesis to PHA, we assure that the precipitation contains immunosuppressive factors. We treated eight cancer patients and there were improvement in general conditions in four patients, however tumoricidal effect observed in one patient. Removal of immunosuppressive factors from cancer patients is essential and an appropriate combination therapy may be needed to achieve effective result.
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[in Japanese]
1986 Volume 15 Issue 3 Pages
1625
Published: June 15, 1986
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S. MATSUBARA, Y. ASANUMA, S. OMOKAWA, K. OKABE, T. FUJIYA, K. ONCHI, Y ...
1986 Volume 15 Issue 3 Pages
1626-1628
Published: June 15, 1986
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For the past 21 months, 356 sessions of plasma exchanges were carried out for33patients using Cobe Centry TPE system. No sereous side effects such as profound hypotension, hemolysis and allergic reaction was observed during procedure. with 50 to 120ml/min of blood flow rate, the system gained a high plasma separation rate averaging 34ml/min. Chronic repeated plasma exchanges fpr 4 patients demonstrated a good biocompatibility of the system evidenced by little changes of blood cellular elements. In addition, the chronic weekly plasma exchange with one plasma volume or more of PPF solution did not produce any unbalance of coagulation status.
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M UENO, H SUZUKI, T OHNO, I OHHAMA, T SHIMOYAMA, Y SAWADA, S NISHIMURA
1986 Volume 15 Issue 3 Pages
1629-1632
Published: June 15, 1986
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Effects of Venopirin in hollow fiber type membrane plasma separator were discussed. 14 patients with rheumatoid arthritis, systemic lupus erythematosus and malignant exophthalmos were treated by double filtration plasmapheresis (DFPP) Transmembrane pressure (TMP) increased over 50mmHg in 4 patients. A difference between inlet and outlet pressure (ΔP) also increased. However, TMP and ΔP decreased after injection of Venopirin in the 4 patient. This result indicates that Venopirin is effective in the cases of plugged up membrane plasma separator.
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K. MASTUDA, T. OKA, K. HANAZAWA, T. YOSHIOKA, Y. ENDO, T. TANI, M. KOD ...
1986 Volume 15 Issue 3 Pages
1633-1636
Published: June 15, 1986
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Evaluation is made of a fundamental and clinical study the newly developed hollow fiber plasma separator, Plasma Star (PM50). The Plasma Star hollow fiber consists of cellulose triacetate and has wall thickness of 80μm, an internal diameter of 270μm, and a maximum pore size of 0.4μm. Its effective surface area is 0.5m
2. In the fundamental study, transmembrane pressures were stable at 8-13mmHg throughout the perfusion, during which the blood and plasma flow was 100ml/min and 35-38ml/min, respectively. Sieving coefficients of T. P., albumin, gamma-globulin and beta-globulin were 0.95 in each case. In the clinical study, eleven patients were treated 32 times for over 2 hours. The sieving coefficients of albumin, IgG, IgA, and IgM were 91%, 91%, 94%, and 98%, respectively. Mean decrease in leukocyte and platelets during plasma exchange were 4% and 10%. Red blood cell counts remained at the same level. There were no differences on the data of blood chemistry between before and after plasma exchange.
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H. TSUDA, M. YOKOYAMA, M. SATO, J. YAMAGATA, H. HASHIMOTO, S. HIROSE
1986 Volume 15 Issue 3 Pages
1637-1640
Published: June 15, 1986
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Since Wallace reported the efficacy of lymphocytoplasmapheresis (LPP) in the treatment of rheumatoid arthritis (RA), it has been tried to many patients with HA. In this study, by using of leukocyte removal filter (Asahi “Sepacell”) lymphocytapheresis (LCP) had been carried out to systemic lupus erythematosus (SLE) and LPP also carried out to SLE by use of “Sepacell” and centrifugation. 11 SLE who showed lupus nephritis and resistance to conventional treatments, were selected for these apheresis. LCP might be effective and LPP could not neccessarily be non-effective.
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M. ICHIWATA, N. NEGISHI, H. HAGIWARA, S. OZAKI, Y. ISHII, S. NIINO, H. ...
1986 Volume 15 Issue 3 Pages
1641-1645
Published: June 15, 1986
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In order to prevent of myonephropathic metabolic syndrome due to acute arterial occulusions. The new plasmafilter is a hollow-fiber type made of polypropylene membrane. We estimated its performance in the animal experiment using mongrel dog. In this result, potassium and myoglobin is decreased in serum, and this treatment of MNMS is clinicary useful.
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-with special reference to phagocytic activity of Kupffer cells-
Y. ASANUMA, S. MATSUBARA, K. OUCHI, K. NAKAGAWA, T. SATO, S. OMOKAWA, ...
1986 Volume 15 Issue 3 Pages
1646-1649
Published: June 15, 1986
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The function of Kupffer cells was examined before and after PE with CSFe and phagocytic index (K) was calculated. K decreased clearly just after PE. The possible reason is such that Kupffer cells pick up IC and other solutes during PE (augmented removal), resulting in transient hypofunction just after PE. When FFP was used as a replacement fluid, fibronectin was supplied to the patient and phagocytic activity of RES seemed to be maitained.
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J. MINAKUCHI, K. TAKAHASHI, S. TERAOKA, H. TOMA, Y. HAYASAKA, T. AGISH ...
1986 Volume 15 Issue 3 Pages
1650-1653
Published: June 15, 1986
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Plasmapheresis was applied in nine highly sensitized patients awaiting kidney transplantation in an attempt to remove pre-formed antibodies prior to transplantation, Nine patients were selected on the basis of the presence of pre-formed antibodies which had been present for more than 6 months. The patients underwent standard plasma exchange (2l×4-6) or double filtration plasmapheresis using plasma membrane separators. After the treatment, cross-match testing in six of the nine patients showed negative, and kidney transplantation has been performed on five of the nine patients. These patients have undergone followed-up treatmentfor a period ranging from 7 to 46 months after the transplantation. Four patients have a functioning graft, with sernm creatinine levels ranging from 13 to 55mg/dl As theopportunities for cadaveric kidney transplantation is limited in our country, this approach provides hope for the large number of highly sentized patients awayting kidney transplantation.
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[in Japanese]
1986 Volume 15 Issue 3 Pages
1654
Published: June 15, 1986
Released on J-STAGE: October 07, 2011
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Y. HAYASAKA, T. AGISHI, M. MINESHIMA, S. TERAOKA, K. TAKAHASHI, H. TOM ...
1986 Volume 15 Issue 3 Pages
1655-1658
Published: June 15, 1986
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Interleukin-1, -2 (IL-1, IL-2) were well known as enhancing factors in immune response system. Ciclosporin (CYA) is a new potent immunosuppressive agent to allo-transplantation of kidney, liver and heart etc. The immunosuppressive effects of CYA was mainly inhibition of the synthesis of IL-1, IL-2. But, after the synthesis of IL-2, the immunosuppressive effect of CYA to immune response system was decreased sharply.
In this study, immunological adsorptive capability has been screened in 115 nonbiological materials by bioassay to IL-2 using IL-2 sensitive culture cell line (NKc-7).
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Y. SASAKI, H. OGIWARA, M. MINESHIMA, Y. HAYASAKA, T. SUZUKI, T. AGISHI ...
1986 Volume 15 Issue 3 Pages
1659-1662
Published: June 15, 1986
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In cases of the transplantation between donor and recipient incompatible in ABO blood-group, elimination of ABO blood-group antibodies is expected. BIO-SYNSORBS adsorb and remove of anti-A and anti-B blood-group antibodies from biological media. The results obtained in our experiments indicates those specific adsorption properties.
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-The specific characteristic of IgM adsorption-
Y. YAMAMOTO, T. SAMESHIMA, A. TAKAHASHI, T. AKAIKE
1986 Volume 15 Issue 3 Pages
1663-1667
Published: June 15, 1986
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We studied the role of ligand (sulfathiazole), spacer (diamine corn-pounds) and pore size (300-2400Å) to the adsorption of plasma proteins in the preparation of chemically modified silica gel beads for immunotherapy. With an increase in surface area, the adsorption of albumin, globulin, IgG and IgA showed a tendency to increase. IgM and aggregated human IgG of high molecular weights, however, had the reverse tendency. The adsorption of albumin increased to some extent with the length of the ligand-spacer. Moderate length spacers indicated the highest value in the selective adsorption of IgM. Consequently, we found that the selectivity of adsorption was affected by the molecular sieving effect as well as the specific characteristic of the ligand. And it seems that an adsorbent having a moderate ligand-spacer and pore size is useful for the removal of higher molecule immunoglobulins in therapy.
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-Analysis by the column method-
T. SAMESHIMA, Y. YAMAMOTO, A. TAKAHASHI, T. AKAIKE
1986 Volume 15 Issue 3 Pages
1668-1671
Published: June 15, 1986
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We studied the adsorptive characteristics of several adsorbents for plasma proteins with the column method. Good results were obtained for selective adsorption of plasma proteins in the adsorbent where the ligand is sulfathiazole and the carrier is acrylic beads (Eupergit-C) or silicagel beads (pore size 906Å). Eupergit-C-ST was particularly suitable for selective adsorption of IgG as was Silicagel-ST for IgM. Washing the adsorbent with rinse solution after plasma perfusion also improved the adsorptive selectivity of globulin. We prepared a prototype column packed with the immunoadsorbent and performed ex vivo perfusion test. And we evaluated in vitro test and ex vivo result. We conclude that the newly developed immunoadsorbents are promising for immuno therapy.
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J. MIURA, A. USUBA, H. INOUE, Y. ENDOH, A. OHISHI, R. MOTOKI
1986 Volume 15 Issue 3 Pages
1672-1675
Published: June 15, 1986
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PFC has recently developed as an artificial blood. But because of the form of emulsion, it may taken up by macrophages in RES and it may cause immune suppression. In this study, immunosuppressive effects of the PFC and its utilization to the skin grafts were investigated: 1) PFC markedly suppressed the immune reactivities of T lymphocytes by suppressing the Interleukin-1 production by macrophages. 2) PFC administration prolonged the graft survival of the mouse skin grafts. We conclude that PFC caused chronic immune suppression by inactivating of the macrophage function and it can be utilized as an immunosuppressive agent in skin graft.
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J. HIRAOKA, T. AKAIKE
1986 Volume 15 Issue 3 Pages
1676-1679
Published: June 15, 1986
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Recently there are many applications for diagnosis and therapy using immunoglobulin. In this study we examined applications for ‘immunoengineering system’ using the immobilized immunoglobulin G (IgG). Polymethyl methacrylate (PMMA) was chosen as matrixes, and the side chain was variously substituted. And the optimal IgG adsorption by the design of polymer surface was examined. It was found that the affinity of Fab site to PMMA was a little higher than that of Fc, whereas, once the ester, group was replaced by carboxyl group (-COON) that of Fc site was higher. And it was also found that the changes of the environments around -COON by introducing various amino acids were effective to the control of IgG adsorption.
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[in Japanese]
1986 Volume 15 Issue 3 Pages
1680
Published: June 15, 1986
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H. ICHIKAWA, K. ICHIKAWA, N. INOUE, Z. YAMAZAKI, Y. IDEZUKI, Y. FUJIMO ...
1986 Volume 15 Issue 3 Pages
1681-1684
Published: June 15, 1986
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A polyolefine MPS (effective surface area: 0.2m
2) for donor plasmapheresis has been developed and evaluated from the views of performance. and safety. 400ml of plasma collection from 12 donors was performed. Plasma flow rate was 13-15ml/min at 50ml/min of blood flow rate. Sieving coefficients of various plasma proteins showed more than 0.95. No free hemoglobin was detected in collected plasma. The concentration of complements in donor's plasma did not show a significant change.
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K. ICHIKAWA, H. ICHIKAWA, N. INOUE, M. HIRAISHI, F. KANAI, Z. YAMAZAKI ...
1986 Volume 15 Issue 3 Pages
1685-1688
Published: June 15, 1986
Released on J-STAGE: October 07, 2011
JOURNAL
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Donor plasmapheresis was satisfactorilly carried out using a cellulose tri-acetate membrane plasma separator and FUT-175 as an anticoagulant. After the plasma was frozen and preserved for 2 weeks, it was defnoted and infused safely into a dog without any side effect.
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H. IKEDA, H. HAYAMIZU, T. TOMONO
1986 Volume 15 Issue 3 Pages
1689-1692
Published: June 15, 1986
Released on J-STAGE: October 07, 2011
JOURNAL
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Small area membrane plasma separators for donor plasmapheresis were evaluated from the views of performance and safety of modules. The stable plasma separation could be achieved at a plasma filtrate rate (Q
F) of 15ml/min for plasma separators with membrane area of 0.25 to 0.30m
2 The sieving coefficients and recoveries of blood coagulation factors were lower than those of the other proteins such as albumin, IgG, etc. The recovery of factor VIII activity was only 0.6 in average. Complement activation was detected with all plasma separators. More effective and safer modules should be developed for the donor plasmapheresis.
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-Comparison between centrifuge and membrane methods.-
M TAMURA, H MATSUDA, [in Japanese], M KASAI, N ENDO, S AKAISHI
1986 Volume 15 Issue 3 Pages
1693-1697
Published: June 15, 1986
Released on J-STAGE: October 07, 2011
JOURNAL
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From April 1986. practical application of donor plasmapheresis (DP) will be governmentally approved in Japan. To popularize DP, donor safety or acceptability seemed to be recovery rates of blood components and adverse effects found in donors after DP treatments. Among the factors, the recovery rates were examined on volunteers, 170 males and 92 females in respect of methods of plasma collection (centrifuge and membrane) and sex distinction. The recovery rates were different according to the components and the results can be classified into 4 groups. The recovery ratio exceeding 98 in almost all the components were observed a week after DP treatments irrespective of the methods or sex distinction. Significant difference of the recovery ratio regarding main proteins between the both methods was never found. Significant difference of the ratio concerning the sex distinction was also never experienced and seemed to be equivalent. Each recovery production rate/day of protein components after DP treatments calculated from the recovery ratio is; TP 7.4%, Albumin 8.2%, IgG 4.0%, IgA 5.1%, and IgM 11.0%, In conducting DP treatments, it is important to take care of some matters such as detail explanation, on DP treatments prior to the initiation, checking of donor conditions and post DP consideration on donors.
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[in Japanese]
1986 Volume 15 Issue 3 Pages
1698
Published: June 15, 1986
Released on J-STAGE: October 07, 2011
JOURNAL
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