8-Hydroxydeoxyguanosine (8-OH-dG) was formed in deoxyguanosine (dG) solution by the addition of Fe
2+, ascorbic acid (AscA), EDTA, and H
2O
2, and was then detected by ECD after separation by HPLC. 8-OH-dG was not formed in significant amounts when either Fe
2+, AscA, or H
2O
2 was added separately, or when two of these were added in combination. When Fe
2+, AscA, and H
2O
2 were added simultaneously, however, 0.87nmol of 8-OH-dG was formed from 2.5μmol of dG. And the addition of EDTA (in the same concentration as Fe
2+) to the Fe
2+/AscA/H
2O
2 system caused a remarkable increase in the amount of 8-OH-dG formed (up to 2.89nmol from 2.5μmol of dG). The amount of 8-OH-dG formed increased as the concentration of AscA increased up to 1mM (the same concentration as Fe
2+). However, higher concentrations of AscA inhibited the formation of 8-OH-dG.
抄録全体を表示