Two Shiga toxin (Stx) genes (
stx-1 and
stx-2) were cloned and sequenced from a strain of enterohemorrhagic
Escherichia coli, serotype O157:H7, a clinical isolate from the Gifu City, Japan, outbreak in 1996 (GPU96MM). The 1, 257 by of
stx-1 and 1, 321 by of
stx-2 genes were analyzed, and each gene contained two open reading frames (ORFs), 948 or 960 by of A and 270 by of B subunits, respectively. Although the nucleotide sequences of
stx-1 ORFs of this isolate had several nucleotide alterations, the deduced amino acid sequence was identical to that of
stx-1 from
E. coli (O157:H7) isolated from Canada and stx from
Shigella dysenteriae type 1. The nucleotide sequences of the
stx-2 ORFs of this strain were identical to those of
stx-2 from two
E. coli (O157:H7) strains, KNIH317 isolated from Korea and 933.
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