It is very important to understand the pigment particle size for the study to promote the quality of pigments. For the anaysis of pigment particle size, it is necessary to make the suspension of particles dispersed perfectly. And when the suspension is diluted with solvent, it should never aggrigate. By dispersing the pigment into alkyd resin varnish with the dispersing agent (I), We could obtain the non aggregated suspension. The suitalbe amount of the dispersing agent was about 10% of the base pigment. CuPC-SO2N(CH2)3N(C2H5)2 (I) The mean diameter measured by the Sub-micron Particle Analyzer (COLUTER Model N4) was correlated to the one calculated by volume average from means of length and width of each particle in transmission electron micrographs. We estimated that the difference between two diameters shows the adsorption layer. In case of C. I. Pigment Blue 15 : 3, its thickness was about 10 nm.
A fluorescent pyrenacyl dye was synthesized from a microbial biosurfactant and the fluorescent properties as the amphipathic probe were investigated. The Sugar-lipid type fluorescent dye (I), Pyrenacyl ester of rhamnolipid B (RB-PE) was synthesized by the reaction of rhamnolipid B potassium salt with I-bromacetylpyrene in the presence of I8-crown-6 as a catalyst. Dye (I) was evaluated as fluorescent probes for membrane fluidity or potential. Four kinds of fluorescent patterns were clearly observed in various solvents, micells and liposomes with well reflected the difference in locaton and microenvironment around the RB-PE molecules.