Japanese Journal of Medical Science and Biology
Online ISSN : 1884-2828
Print ISSN : 0021-5112
ISSN-L : 0021-5112
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Volume 23, Issue 4
Displaying 1-7 of 7 articles from this issue
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  • CYTOTOXIC EFFECTS OF SCIRPENE COMPOUNDS, FUSARENON-X PRODUCED BY FUSARIUM NIVALE, DIHYDRONIVALENOL AND DIHYDROFUSARENON-X, ON HELA CELLS
    KOHICHIRO OHTSUBO, MAMORU SAITO
    1970 Volume 23 Issue 4 Pages 217-225
    Published: 1970
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.23.217
    JOURNAL FREE ACCESS
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    Three toxic substances related to nivalenol were tested for their growth inhibiting activity and suppressive effects on DNA, RNA and protein syntheses of HeLa S3 cells in comparison with those of nivalenol.
    Fusarenon-X, isolated from culture filtrate of Fusarium nivale, showed a complete inhibition of growth of HeLa S3 cells at a concentration of 0.5 μg/ml. The ED50 for 3H-thymidine incorporation into DNA and 3H-leucine into protein were 0.1 and 0.13 μg/ml (2.8 and 3.6×10-7M), respectively, thus indicating about a 4 times potent activity as compared with that of nivalenol. No inhibi-tion of 3H-uridine incorporation into RNA occurred.
    Dihydronivalenol and dihydrof usarenon-X, -9-ene reduced substances of nivalenol and fusarenon-X, respectively, showed similar toxic effects on HeLa cells but with a six to ten times lower toxicity. The complete growth inhibition doses for the cells by both samples were around 3.0 μg/ml and the ED50 for 3H-thymidine and leucine incorporation were around 2.0 μg/ml. 3H-uridine incorporation was not remarkably inhibited.
    Biological effects of some other Fusaria metabolites having closely related chemical structures are discussed in association with nivalenol and f usarenon-X.
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  • EFFECT OF TRYPSIN ON VIRAL SUSCEPTIBILITY OF VERO CELL CULTURES-CERCOPITHECUS KIDNEY LINE
    HEIHACHI ITOH, YOSHIO MORIMOTO, ISAO IWASE, YUTAKA DOI, TSUNEO SANPE, ...
    1970 Volume 23 Issue 4 Pages 227-235
    Published: 1970
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.23.227
    JOURNAL FREE ACCESS
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    Addition of dilute trypsin to the maintenance medium of Vero cell cultures made them far more susceptible to certain strains of parainfluenza type 1 (HVJ) and influenza A and B viruses. These findings are in line with those already reported on parainfluenza type 1 (HA-2) and type 4 viruses. To other virus strains belonging to myxo, picorna, reo, arbo, adeno, herpes, papova and poxvirus groups, the susceptibility was not enhanced by this manipulation. The difference of infectivity titers between those measured in Vero cell cultures under the trypsin-added condition (Vrt) and the ordinary ones (Vro) was taken as a criterion for the enhancement. In the serial passage tests of selected viruses in Vrt, most of viruses with the above titer-enhancement was proved transferable in the trypsin-added system, but remained nontransferable in Vro even after the passages in Vrt. Consequently, a possible emergence and selection of variants specially adapted to Vero cells is unlikely to occur in the Vrt system. Therefore, the present observations were considered on the basis of modified viral susceptibility of the cell culture.
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  • SIGNIFICANCE OF THE INCIDENCE OF SHIGELLA INFECTION AS INDICATED BY RESULTS OF A LONGITUDINAL STUDY
    PREMA BHAT, RUTH M. MYERS, ROGER A. FELDMAN
    1970 Volume 23 Issue 4 Pages 237-242
    Published: 1970
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.23.237
    JOURNAL FREE ACCESS
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    A total of 1602 routine rectal swab specimens and 341 diarrhoeal specimens were obtained over a period of one and a quarter years from a group of children of semi-urban areas in the town of Vellore, North Arcot District, Tamil Nadu, India.
    Shigellae were found in 16.1% of the specimens obtained at the time of diarrhoeal episodes.
    A 4.9% incidence of asymptomatic infection with Shigella species was detected, with indication of a true passive carrier state in 3.0%.
    The carrier-case ratio showed a significant difference and established the aetiologic significance of the Shigella group in juvenile diarrhoea.
    Shigella carriers were not found among babies 0-6 months old, but were found with increasing frequency with increase in age, in children of both sexes.
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  • AN OUTBREAK OF TYPE B BOTULISM OCCURRING IN MIYAZAKI PREFECTURE
    TAKEO FUKUDA, TADATOSHI KITAO, HIROTOSHI TANIKAWA, GENJI SAKAGUCHI
    1970 Volume 23 Issue 4 Pages 243-248
    Published: 1970
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.23.243
    JOURNAL FREE ACCESS
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    An outbreak of type B botulism occurred in Miyazaki Prefecture in August, 1969. There were 21 cases of which three resulted in death of patients. This was the first outbreak of human botulism of a type other than type E in Japan. Type B toxin was demonstrated in fecal specimens; Clostridium botulinum type B was isolated from a single patient. Bottled caviar imported from West Germany was most strongly suspected of being the vehicle.
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  • DYSENTERY PRODUCED BY ORAL ADMINISTRATION OF THE COLONIC CONTENTS OF CYNOMOLGUS MONKEYS INFECTED WITH SHIGELLA FLEXNERI 2a
    MASAO TAKASAKA, SHIGEO HONJO, KIYOSHI IMAIZUMI, HIDEMASA OGAWA
    1970 Volume 23 Issue 4 Pages 249-254
    Published: 1970
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.23.249
    JOURNAL FREE ACCESS
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  • TRANSOCEANIC FLIGHT OF MOSQUITOES ON THE NORTHWEST PACIFIC
    SYOZIRO ASAHINA
    1970 Volume 23 Issue 4 Pages 255-258
    Published: 1970
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.23.255
    JOURNAL FREE ACCESS
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  • SYMPOSIUM ON TOXINS
    1970 Volume 23 Issue 4 Pages 259-293
    Published: 1970
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.23.259
    JOURNAL FREE ACCESS
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