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Volume 46, Issue 2
Displaying 1-5 of 5 articles from this issue
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Yoshiharu SUZUKI, Chieko MATSUMOTO, Junnosuke WATANABE, Kusuya NISHIOK ...1993 Volume 46 Issue 2 Pages 57-74
Published: 1993
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSThe presence of an antibody which reacts strongly at lower temperatures to C100-3 antigen derived from the hepatitis C virus (HCV) was demonstrated in blood donors'sera. These sera reacted with the antigen most strongly at 4 C and most weakly at 37 C and are termed ‘cold antibody group’. The cold antibody group reaction was specifically inhibited with soluble C100-3 antigen. Cold antibody bound to C100-3 antigen at 4 C was disassociated at 37 C and was isolated in this manner. Low temperature-dependent anti C 100-3 antibody was shown to be predominantly IgG3 subclass by ELISA using anti-human IgG subclass antibody. This IgG3 antibody was dissociated from antigen with 6M urea, demonstrating its low avidity. HCV-RNA was demonstrated in two of five samples. Therefore, to achieve more effective prevention of post-transfusion hepatitis C, we propose the incorporation of programs to detect low temperature-dependent low-avidity antibody against HCV-related antigen.View full abstractDownload PDF (2728K) -
Kazuyoshi SUGIYAMA, Takashi KITAMURA, Toshihiko KOMATSU, Kiyomi MINABA ...1993 Volume 46 Issue 2 Pages 75-86
Published: 1993
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSThe prevalence of antibodies against hantavirus in 413 rats (Rattus norvegicus) captured in eight regions of the Tokyo Bay area from 1983 to 1992 were examined by the indicrect fluorescent antibody test. Antibody-positive rats were found in Tokyo Port in 1983 (34.8%), 1984 (25.9%), 1985 (22.0%) and 1986 (15.6%), in Kasai Seaside Park in 1989 (3.2%) and 1990 (4.2%) and in Chiba Port in 1990. (6.7%) . In Tokyo Port, antibody-positive rats were found in any season in 1984 and 1985, and seroprevalences among two age groups, i. e., less than six months and six or more months of age were 18.1% and 28.4%, respectively. Three virus strains were isolated from rats captured on reclaimed land No. 13 in Tokyo Port in 1985 and named TQR-23, TQR-48 and TQR-50. These strains were antigenically identical with other rat strains, e. g., strain SR-11 and TR-352 but were discriminated from 76-118 strain by the neutralization test. Until 1990, persistence of hantavirus among rats inhabiting the Tokyo Bay area was demonstrated and hantavirus may be continuously prevalent in rats in this area.View full abstractDownload PDF (993K) -
Huiling HU, Dexiang DONG, Enhua TANG, Ming ZHANG, Yiyun CAO1993 Volume 46 Issue 2 Pages 87-93
Published: 1993
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSAll the 16 mouse monoclonal antibodies (McAbs) raised against Nancy strain of coxsackievirus B3, CoxB3 (N), were type 3-specific. The reactivity of CoxB3 (N) to this McAb panel during the serial passages showed a variation. Three field isolates of CoxB3, all neutralizable with the reference Anti-CoxB3 hyperimmune serum, were checked for the reactivities with the McAb panel. The isolates from the patients in Fujiang reacted with 12 McAbs and variably with the remaining four McAbs. On the basis of the reactivities with different virus strains, the McAbs were found to fall into four groups possibly corresponding to at least four epitopes. A considerable antigenic variation of CoxB3 was thus suggested.View full abstractDownload PDF (568K) -
Hideaki TSUCHIE, Takashi KURIMURA, Namiko YOSHIHARA, Takashi KITAMURA, ...1993 Volume 46 Issue 2 Pages 95-100
Published: 1993
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSThe third variable (V3) domain of human immunodeficiency virus type 1 (HIV-1) env gene has been found to elicit type-specific neutralizing antibodies as well as a cytotoxic and helper T-cell response in both humans and animals. We analyzed the V3 domain of 8 HIV-1 isolates from India by using polymerase chain reactions. The V3 loops of 7 Indian isolates contained the apical tetra peptide GPGQ, while the V3 loop of one Indian isolate carried the apical tetra peptide-GPGK. The amino acid sequences of the seven Indian isolates were closely related to each other, with an average of the nucleotide sequence homology of 96.0% (94.6 to 97.6%) . The marked relatedness of the amino acid sequences among the seven Indian HIV-1 isolates indicated a recent and very rapid spread of this HIV-1 variant in Bombay. The amino acid sequence of the C2/V3 region of env gene of the 7 Indian isolates were homologous to the C subtype reported by Meyers et al. These findings could be useful in assessing the sources of infection and developing an AIDS vaccine.View full abstractDownload PDF (545K) -
1993 Volume 46 Issue 2 Pages 101
Published: 1993
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSDownload PDF (42K)
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