We investigated the nature of a normal human serum factor (s) precipitating bovine enterovirus, and obtained the following results. (1) The incidence of the precipitating factor in human serum increased greatly at the ages between 2-5. (2) The precipitating factors in most of the sera examined were identified as IgG globulin. (3) A bovine enterovirus was found to possess two distinct antigens resembling N (native) and H (heat stable) antigens of human enteroviruses ; normal human serum reacted exclusively with the latter. (4) The majority of human sera precipitating heat stable antigen of bovine enterovirus also reacted with the same antigens of human enteroviruses, coxsackievirus B1, coxsackievirus B5 and echovirus type 1. (5) Absorption experiments of human serum with heated viruses indicated the presence of a common antigen (s) between the heat stable antigen of bovine enterovirus and those of coxsackie- and echoviruses, while no serological relationship was found between the heat stable antigen of bovine enterovirus and those of polioviruses.
From these results it was presumed that the human serum factor precipitating bovine enterovirus is possibly an antibody produced by infection with coxsackie or echoviruses sharing a common antigen (s) with bovine enterovirus.

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During the exeriments on the biochemical influence of monosodium glutamate on rats, Pseudomonas aeruginosa infections broke out affecting 23 of 95 rats, 45 males and 50 females, after long-term feeding with diets containing 5 to 20 % monosodium glutamate. Of the 23 infected animals, five males and 18 females, two males and 17 females had lesions in the lungs, mainly lung abscesses. Other changes observed were hemorrhagic enteritis, suppurative keratitis, suppurative prostatitis, and cerebellum abscess. Of 23 control rats autopsied, only one male had subcutaneous abscess in the abdominal region. A large number of P. aeruginosa organisms were found in the jejunum of rats with lung infections. P. aeruginosa of two particular pyocine types were most frequently recovered from the lesions and intestines of the diseased rats. The results of pyocine typing suggested auto-infection. The possible relationship between P. aeruginosa infection and MSG feeding, age and sex was discussed.

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Effects of mixing living BCG with living tumor cells on the growth of the tumor cells in the intradermally inoculated mice and induction of the tumor immunity were investigated with various syngeneic and allogeneic mouse tumor systems. Tumor immunity was assessed from transplant resistance against challenge tumor grafts, macrophage migration inhibition and peritoneal macrophage disappearance. The effects of BCG-tumor cell mixtures varied depending upon the tumor lines and also upon the experimental conditions employed. From the modes of the effect, the tumors used were classified into the following three types: (1) rejecting the primary tumor grafts and inducing the tumor-specific, cell-mediated immunity; (2) rejecting the primary tumor grafts but inducing no detectable tumor immunity; and (3) not rejecting the primary tumor grafts even in BCG-sensitized mice. These types seemed to correlate with the levels of antigenicity and the growth rates of the tumor cells. Number of the tumor cells in the inoculum, in addition to the tumor lines, largely influenced the effects. For the optimal suppression of the growth of tumor cells in the primary inocula, generally required were the following factors : (1) prior sensitization of mice to BCG, (2) direct contact between BCG and tumor cells, and (3) intradermal route of injection.

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Mitraspora cyprini was found in the diseased kidney or ureter or both of goldfish, most of which were collected seasonally from representative goldfish farms in Edogawa Ward, Tokyo. Histological observations of both healthy and diseased kidneys were made. Observations were also made of smeared and stained preparations and living specimens of M. cyprini.
The spore is oblong with slightly attenuated anterior end. Each of the two shell valves bears eight striae. It has eight caudal filaments, two polar capsules and a binucleate amoebula.
The trophozoites multiply in the renal tubules by the processes of plasmotomy and gemmation and infect the epithelial cells. The trophozoites are polysporous and the spore is monosporont.
Though this Mitraspora differs slightly in taxonomic features from the previous authors', the author identified it as M. cyprini.
Infection takes place through the mouth. The liberated binucleate amoebulae, penetrating the wall of the small intestine, reach the tubules through glomerules by blood. Intracellular and intraluminar life follow multiplication and sporogony in the tubules. The whole life cycle takes a year to complete.

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