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Volume 25 (1972)
- Issue 6 Pages 355-
- Issue 5 Pages 291-
- Issue 4 Pages 249-
- Issue 3 Pages 133-
- Issue 2 Pages 57-
- Issue 1 Pages 1-
Predecessor
Volume 25, Issue 2
Displaying 1-7 of 7 articles from this issue
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J.L. PADRON, CLAYTON HUNT, NOEL LEWIS1972 Volume 25 Issue 2 Pages 57-62
Published: 1972
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSThe effect of glucosamine-acetoacetate condensate (pyrrolecondensate) upon insulin and glucose plasma levels of white albino rats was determined. Parallel experiments were made upon the rate of incorporation of uniformly labeled glucose-C-14 into liver and muscle tissue.
Our results show that pyrrole-condensate is capable of significantly increasing the levels of insulin and reducing the levels of glucose, in the serum of white albino rats. The incorporation of glucose into liver and muscle was substantially increased in the pyrrole-treated rat as compared with the normal.View full abstractDownload PDF (466K) -
KEIU NINOMIYA, FUMISHIGE OHTANI, SUNAO KOOSAKA, HARUKO KAMIYA, KAZUE U ...1972 Volume 25 Issue 2 Pages 63-73
Published: 1972
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSAbout 250 strains of the three genera were examined and a new and very simple method was devised for their differentiation. Plates of “Bacteroides” medium (colistin, neomycin and brilliant green) and “modified FM” medium were streaked with the strains and incubated for 48 hr. Almost all strains of Bacteroides grew well on the former but not on the latter. Almost all strains of Sphaerophorus and Fusobacterium grew well on the latter but not on the former. These results were very clear-cut and conformed to the production of n-butyric acid in more than 90%. Other characteristics determined by the Taxonomic Subcommittee for gram-negative anaerobic rods were examined. The followings were found to be reliable and simple to perform: final pH in glucose medium, glutamic acid decarboxylation and threonine dehydration. Discussions were given and the “Bacteroides” and “modified FM” plate method was recommended as the most simple, reliable and time-saving method for clinical bacteriological laboratories.View full abstractDownload PDF (1177K) -
AKIRA WAKE, HIDEMI MORITA, MAKOTO YAMAMOTO1972 Volume 25 Issue 2 Pages 75-84
Published: 1972
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSThe effect of iron administration on experimental plague infection was studied with colloidal iron chondroitin sulfate. The mice inoculated subcutaneously with an attenuated plague strain took the fatal outcome when they were treated with the drug; the resulted histopathological changes were similar to those of the animals infected with a virulent strain. An early appearance of clumped bacilli, parenchymal cell infiltration, granuloma and necrosis in the liver, and atrophy of red pulp, hyperplasia or necrosis or both of follicles in the spleen and lymph nodes were the main findings in the iron-administered mice. In contrast to these, the iron-untreated mice showed only perivascular lesions in the liver and slight changes in the spleen. The drug administration suppressed the development of immunity in mice in response to a protective antigen, Fraction I. Additional experiments demonstrated that the drug has no toxicity-increasing effect on the murine toxin. From these observations, the “virulence-enhancing” effect of iron can be explained as an interfering with the host defense mechanism.View full abstractDownload PDF (3165K) -
HIROKO TSUKANO, MAKOTO YAMAMOTO, AKIRA WAKE1972 Volume 25 Issue 2 Pages 85-93
Published: 1972
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSThe effects of iron on the growth of plague microorganisms were examined with broth and agar media of deironized brain heart infusion added with or without chondroitin sulfate ferric chloride. The iron-deficiency inhibited growth-initiation of P- attenuated vaccine strain EV of Yersinia pestis, but affected slightly the generation time in the later growth. Such a high dose as 3 mg Fe/ml was, however, inhibitory to the strain. The substrains selected f ram the colonies grown on an iron-deficient medium acquired a partial tolerance to such environmental condition. Hyperimmune antiplague rabbit serum inhibited the growth of EV microorganisms, which was removed by addition of more than 0.5 mg Fe/ml of the iron drug. Four mg Fe/ml was still a growth-stimulating concentration in the HAPS- containing environment.
When EV microorganisms were introduced into guinea pig skin together with 0.1 ml of the iron drug containing 0.4 mg Fe, viable counts increased from 104 to 107 within 2 days and the same level persisted at least for 1 week.View full abstractDownload PDF (2004K) -
KIYOTO AKAMA, SHO OTANI, AKIHARU ITO1972 Volume 25 Issue 2 Pages 95-104
Published: 1972
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSThe rabbit skin treated with antibacterial serum against nontoxigenic Corynebacterium diphtheriae showed perfect protection from the challenge with toxigenic organisms, whereas the skin of the rabbit immunized actively with non-toxigenic organism showed only partial resistance to the challenge. Protection became complete, however, when the skin of such a rabbit had been treated with the autologous immune serum.
For complete protection, an interval longer than 3 hr was required between the administration of antiserum and the challenge. No protection was observed when the serum and the organism were mixed before inoculation. The protective principle in the antiserum appeared to be of globulin nature (mainly IgG) ; the protection was specific for diphtheria bacilli.View full abstractDownload PDF (2213K) -
FURTHER DEMONSTRATION OF BACTERIAL LIPIDS IN MYCOBACTERIUM BOVIS HARVESTED FROM INFECTED MOUSE LUNGSEIKO KONDO, KOOMI KANAI1972 Volume 25 Issue 2 Pages 105-122
Published: 1972
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSMycobacterium bovis grown in vivo were separated by differential centrifugation from homogenate of the experimentally infected mouse lungs and subjected to lipid fractionation by Anderson-Lederer method in comparison with the same strain grown on Sauton synthetie liquid medium. The per cent yield of chloroform-soluble wax was much smaller than that of wax A in the lung bacilli. The esters of mycocerosic acid were the main components of wax A of the in vivo bacilli, but some esters of mycolic acid were not detected unlike the case of the Sauton bacilli. An arabinolipid, probably arabinose mycolate, and tuberculostearic acid-containing cardiolipin were separated from bound lipids and the phospholipid fraction, respectively, as the lipids common in both kinds of bacilli.View full abstractDownload PDF (8046K) -
HISASHI KONDO, SATORU KONDO, SEIJI SADAHIRO, KIYOSUMI YAMAUCHI, AKIRA ...1972 Volume 25 Issue 2 Pages 123-131
Published: 1972
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSA new procedure for determining the amount of venom ejected by a bite of a poisonous snake was established. The procedure consists of exposing a fresh piece of rabbit muscle to an attack by a poisonous snake, extracting the injected venom from the piece, determining the toxic activity of the extract and calculating the amount of venom ejected. We confirmed that about 90 % of the injected venom was recovered.
We found that the average quantity of venom ejected by a single bite of Trimeresurus flavoviridis was 0.0802 ml (13.2 mg dry weight) or about 9.8 % of the total available venom ; the corresponding figures for Trimeresurus el egans were 0.0717 ml (11.4 mg dry weight) or about 6.4%.
The total amount of venom ejected by three successive bites was approximately the same as three times the amount ejected by a single bite. There was an apparent correlation between the body length of the snake and the total available venom.View full abstractDownload PDF (694K)
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