All issues
Successor
Predecessor
Volume 50, Issue 1
Displaying 1-5 of 5 articles from this issue
- |<
- <
- 1
- >
- >|
-
Abdulrahim JAMAL MOHAMED, Durairaj SATHIYARAJ, Ramaswamy MUTHU1997 Volume 50 Issue 1 Pages 1-7
Published: 1997
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSWe have used the cell wall-associated proteins of Mycobacterium tuberculosis (SIHV strain) as antigens in the serodiagnosis of pulmonary tuberculosis. Sera from 19 relapsed and 46 newly diagnosed cases of pulmonary tuberculosis and 21 healthy individuals were tested against the cell wall-associated proteins of M. tuberculosis (SIHV) by an ELISA technique. The results showed 92% and 100% positive titers in new and relapsed cases of tuberculosis, respectively. Control sera analyzed exhibited a negativity of 90%. Cell wall-associated proteins of M. tuberculosis were found to be useful in serodiagnosis of pulmonary tuberculosis in clear distinction from healthy subjects.View full abstractDownload PDF (566K) -
Marimuthu ADHIRAI, Ramasamy SELVAM1997 Volume 50 Issue 1 Pages 9-17
Published: 1997
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSThe effect of cyclosporin A, a highly effective immunosuppressant, was investigated on hyperoxaluric rats with and without vitamin E pretreatment. Hyperoxaluria was induced by oral feeding of 3% ammonium oxalate in water for 3 days. Cyclosprin A (50 mg/kg body wt.) was administered for 3 days. Pretreatment with vitamin E (50 mg/100 g body wt., once a week for 3 weeks) was carried out before the administration of cyclosproin A and ammonium oxalate. Nonenzymatic ascorbate-induced lipid peroxidation was increased to 1.55-fold in either cyclosporin A-administered or hyperoxaluric rat kidney and liver when compared to control. The lipid peroxidation was further elevated to 1.9-fold when both cyclosporin A and ammonium oxalate were coadministered. The activities of renal and hepatic ATPase, glucose-6-phosphatase as well as the concentrations of thiols were decreased significantly (p<0.001) when cyclosporin A was administered under hyperoxaluric condition. On pretreatment with vitamin E the cyclosporin A-induced biochemical changes observed in the presence of hyperoxaluria were abolished.View full abstractDownload PDF (753K) -
Akira ITO, Hao WEN, Philip S. CRAIG, Liang MA, Minoru NAKAO, Toshihiro ...1997 Volume 50 Issue 1 Pages 19-26
Published: 1997
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSWestern blot analysis was carried out in order to evaluate new serodiagnostic markers, Em18 and Em16, for differentiation of alveolar echinococcosis (AE) from cystic echinococcosis (CE) using 36 serum samples from hydatid patients from Xinjiang, China, where AE and CE are both endemic and one double infection case has been reported. All AE cases except one (5/6) who exhibited a calcified lesion and a single case of double infection showed antibody responses against Em18 and Em16. Some of CE patient sera (6/22) showed antibody response against Em16 except one who showed that against Em18. Analyses of IgG subclass responses against Em18 and Em16 were carried out us-ing all serum samples showing antibody responses against Em18 and/or Em16 (seven CE, five AE, and one AE+CE) and additional samples of three CE and 22 AE from Sichuan, China. IgG4 was the most predominant antibody subclass. Em18 and Em16 were recognized by both IgG4 and IgG1 (in most cases) or by either IgG4 or IgG1 (in minor cases) or by IgG3 (in very rare cases) . Neither Em18 nor Em16 was recognized by IgG2 antibodies. The usefulness of Em18 and Em16 as potential new markers for serological differentiation of human AE and CE, respectively, is discussed.View full abstractDownload PDF (733K) -
Hiroshi TSURUOKA, Hong XU, Kazumichi KURODA, Kozaburo HAYASHI, Osamu Y ...1997 Volume 50 Issue 1 Pages 27-34
Published: 1997
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSGastroenteritis, arthralgaia and myalgia are frequently associated with influenza virus infections in humans. One explanation for these symptoms may be extrarespiratory transmission of virus by peripheral blood mononuclear cells (PBMC) . We tried to detect genomic viral RNA of the nucleoprotein (NP) and H3 subtype hemagglutinin (HA) genes by the method of RT-PCR in PBMC of 18 children aged 1-14 who sufferred from an influenza outbreak in the Kansai district of Japan between December 1992 and February 1993. Three of the 18 samples were RT-PCR positive. The NP gene sequence observed in one patient's PBMC was identical to that obtained from his throat swab fluid. The HA gene sequences observed in the two other PBMC differed from those of RT-PCR-amplified DNA from throat swabs by an order of 3-9 nucleotides. We believe these results suggest the presence of a PBMC-associated virus.View full abstractDownload PDF (2340K) -
Shingou SAKURAI, Kazunari KAMACHI, Toshifumi KONDA, Katsutoshi KOMURO, ...1997 Volume 50 Issue 1 Pages 35-43
Published: 1997
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSMice inoculated with whole cell pertussis vaccine (WCV) acquired protection against intracerebral challenge with Bordetella pertussis without any appreciable antibody production against pertussis toxin or filamentous hemagglutinin. Spleen cells from mice immunized with WCV produced a significant amount of IFN-γ upon stimulation in vitro with WCV. Furthermore, mice inoculated with recombinant IFN-γ along with a suboptimal dose of WCV survived longer than those that received WCV alone. These results suggest that, in WCV-immune mice, IFN-γ plays an important role in protection against intracerebral challenge with B. pertussis.View full abstractDownload PDF (734K)
- |<
- <
- 1
- >
- >|