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Volume 47 (1994)
- Issue 5-6 Pages 241-
- Issue 4 Pages 179-
- Issue 3 Pages 127-
- Issue 2 Pages 73-
- Issue 1 Pages 1-
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Volume 47, Issue 5-6
Displaying 1-5 of 5 articles from this issue
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Lathika NAIR, Virendra K. BHASIN1994 Volume 47 Issue 5-6 Pages 241-252
Published: 1994
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSAntiplasmodium properties of cisplatin [cis-platinum (II) diammine dichloride], a neoplastic drug, have been assessed in in vivo and in vitro model systems of malarial parasite. A well-tolerated dose of 6 mg/kg body weight of the compound cured the mice infected with Plasmodium berghei and the amount of cisplatin required for in vitro inhibition (IC50) of a chloroquine-resistant Plasmodium falciparum isolate was smaller than either chloroquine or quinine. The minimum inhibitory concentration (MIC) needed to prevent the in vitro multiplication of asexual blood parasites was 30 ng/ml. Late ring and trophozoite stages of the erythrocytic cycle were the most susceptible, whereas schizont and early ring stages were the least sensitive to the toxic effect of cisplatin. Multiple smaller doses were more effective in curing malaria in mice than a single large dose. In a few of the mice treated with a single intraperitoneal large dose of 6 mg/kg body weight, there was a delay in appearance of parasitemia but most of them recovered completely but slowly. This compound exerts its toxicity mainly by randomly damaging and cross-linking DNA strands as shown by Southern hybridization with a synthetic oligonucleotide probe, which is a repeat sequence in the falciparum genome. The report clearly demonstrates the antimalarial potentials of this compound and suggests a closer evaluation of this and other related compounds, specially in combination with antimalarial drugs to probe their synergistic properties.View full abstractDownload PDF (2425K) -
Chege Joseph MUKURIA, Akira NOGUCHI, Eiko SUZUKI, Masaharu NAIKI1994 Volume 47 Issue 5-6 Pages 253-264
Published: 1994
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSA human Hanganutziu-Deicher (HD) antibody and a chicken anti-N-glycolylneuraminyllactosylceramide (HD3) antibody were compared in their reaction against HD antigen-active ganglioside (HD3) and a glycoprotein (GP) by radioimmunassay (RIA) . The human antibody had a 50 times higher reactivity with the glycoprotein, while the chicken antibody reacted equally with both antigens. Both antibodies had a higher reactivities with HD antigen (s) in sera of five of eight lung cancer patients than 54 normal human sera. Since four of the above five sera had no abnormal titers to GP, it was concluded that their immunological status was antigen excess. The chicken antibody may be useful in follow-up studies of cancer patients to correlate the expression of HD antigen in tissues and sera with the elevation of HD antibodies, offering alternative methods of clinical prognosis of tumor growth and/or metastases. The human HD antibody may also be useful for the detection of HD antigens of glycoprotein nature.View full abstractDownload PDF (1026K) -
Ivan T. RAICHEV, Raina BORISSOVA1994 Volume 47 Issue 5-6 Pages 265-280
Published: 1994
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSProduction of a factor with a biological activity to inhibit the in vitro tumor-cell migration (TCM) from peripheral blood E rosette-forming cells (ERFC), CD4+ and CD8+ T cells in patients with gastric, and breast carcinoma was investigated. The cells were stimulated for 2 or 24 hr with allogeneic gastric or breast cancer extracts in samples of cell suspensions. A microculture system at an initial cell concentration from 2, 500 cells to 1 cell per well was used. Feeder cells, PHA, IL-2-containing supernatant and cancer extract were added to each well. Ehrlich ascites tumor cells were employed in the migration-inhibition assay. ERFC and CD4+ T cells produced in the culture supernatants a factor inhibiting TCM, when these cells were stimulated with cancer extracts, but not with extracts of benign tissue. Stimulated CD8+ T cells did not produce such a factor. The production of the factor inhibiting TCM in the microculture system was also significantly correlated with the type of cells in the wells, particularly with ERFC and CD4+ T cells, but not with CD8+ T cells (r=0.94, p<0.001) . It could be suggested that this factor probably took part in in vivo blockading the migration of tumor cells in small cancer foci.View full abstractDownload PDF (1541K) -
Kazuya HARA, Masanori TOBA, Yoshiaki YOGO, Fumiaki TAGUCHI1994 Volume 47 Issue 5-6 Pages 281-288
Published: 1994
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSTo elucidate the function of the latency-associated transcript (LAT) of herpes simplex virus type 1 (HSV-1) in productive infection and latency, we established a cell line stably expressing LAT (V24 cells) . V24 cells expressed 2.0-kilobase RNA that corresponds to the major species of LAT in mouse and human sensory ganglia. When the cells were infected with HSV-1 at a low multiplicity of infection, the amount of the progeny virus was much smaller in V24 cells than in a control cell line not expressing LAT. Inefficient growth of HSV-1 in V24 cells was also observed when viral replication was initiated by transfection with viral DNA.View full abstractDownload PDF (1872K) -
1994 Volume 47 Issue 5-6 Pages 289-321
Published: 1994
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSDownload PDF (2739K)
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